Adenovirus-Mediated RNA Interference against Foot-and-Mouth Disease Virus Infection both In Vitro and In Vivo

Chen, Weizao; Liu, Mingqiu; Ye, Jiao; Yan, Wei; Wei, Xuefeng; Chen, Jiulian; Liang, Fei; Liu, Yang; Zuo, Xiaoping; Yang, Fugui; Yong-gan, LU; Zhang, Zheng

doi:10.1128/jvi.80.7.3559-3566.2006

Cited by 87 publications

(89 citation statements)

References 36 publications

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“…The resulting adenoviral plasmids (pAd-miR-29a, pAd-miR-29a, and pAd-control) were linearized with PacI, purified by ethanol precipitation, and transfected into HEK293 packaging cells that had been plated into a 25-cm 2 flask the previous day. The cells were transfected with 4 g of linearized plasmid DNA using 20 l of Lipofectamine 2000 reagent (Invitrogen) and monitored for the expression of green fluorescent protein (GFP) (23). After transfection for 12 days, the cells were collected and centrifuged 10 min at 4°C at 500 ϫ g to pellet the cell debris.…”

Section: Methodsmentioning

confidence: 99%

Protective Role of Estrogen-induced miRNA-29 Expression in Carbon Tetrachloride-induced Mouse Liver Injury

Zhang

Wang

et al. 2012

Journal of Biological Chemistry

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Background: Mechanism underlying the protection of estradiol against CCl 4 -induced hepatic fibrosis remains unclear. Results: miR-29 expression was differentially regulated in male/female mice during CCl 4 treatment. Both estradiol and miR29a/b-expressing adenovirus increased hepatic miR-29a/b levels and attenuated CCl 4 -induced hepatic fibrosis. Conclusion: Estradiol inhibits CCl 4 -induced hepatic injury via inducing miR-29a/b. Significance: Learning the role/regulation of miR-29 in hepatic fibrosis and providing novel therapeutic target.

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Section: Methodsmentioning

confidence: 99%

Protective Role of Estrogen-induced miRNA-29 Expression in Carbon Tetrachloride-induced Mouse Liver Injury

Zhang

Wang

et al. 2012

Journal of Biological Chemistry

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“…Previous studies of FMDV have demonstrated that recombinant human adenovirus type 5 expressing siRNA or IFNs can be delivered effectively via i.m. injection in pigs (29,31,36). Therefore, we propose that regardless of the various factors involved, i.m.…”

Section: Discussionmentioning

confidence: 99%

Robust Protection against Highly Virulent Foot-and-Mouth Disease Virus in Swine by Combination Treatment with Recombinant Adenoviruses Expressing Porcine Alpha and Gamma Interferons and Multiple Small Interfering RNAs

Kim

Park

Lee

et al. 2015

J Virol

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Because the currently available vaccines against foot-and-mouth disease (FMD) provide no protection until 4 to 7 days postvaccination, the only alternative method to halt the spread of the FMD virus (FMDV) during outbreaks is the application of antiviral agents. Combination treatment strategies have been used to enhance the efficacy of antiviral agents, and such strategies may be advantageous in overcoming viral mechanisms of resistance to antiviral treatments. We have developed recombinant adenoviruses (Ads) for the simultaneous expression of porcine alpha and gamma interferons (Ad-porcine IFN-␣␥) as well as 3 small interfering RNAs (Ad-3siRNA) targeting FMDV mRNAs encoding nonstructural proteins. The antiviral effects of Ad-porcine IFN-␣␥ and Ad-3siRNA expression were tested in combination in porcine cells, suckling mice, and swine. We observed enhanced antiviral effects in porcine cells and mice as well as robust protection against the highly pathogenic strain O/Andong/ SKR/2010 and increased expression of cytokines in swine following combination treatment. In addition, we showed that combination treatment was effective against all serotypes of FMDV. Therefore, we suggest that the combined treatment with Adporcine IFN-␣␥ and Ad-3siRNA may offer fast-acting antiviral protection and be used with a vaccine during the period that the vaccine does not provide protection against FMD. IMPORTANCEThe use of current foot-and-mouth disease (FMD) vaccines to induce rapid protection provides limited effectiveness because the protection does not become effective until a minimum of 4 days after vaccination. Therefore, during outbreaks antiviral agents remain the only available treatment to confer rapid protection and reduce the spread of foot-and-mouth disease virus (FMDV) in livestock until vaccine-induced protective immunity can become effective. Interferons (IFNs) and small interfering RNAs (siRNAs) have been reported to be effective antiviral agents against FMDV, although the virus has associated mechanisms of resistance to type I interferons and siRNAs. We have developed recombinant adenoviruses for the simultaneous expression of porcine alpha and gamma interferons (Ad-porcine IFN-␣␥) as well as 3 small interfering RNAs (Ad-3siRNA) to enhance the inhibitory effects of these antiviral agents observed in previous studies. Here, we show enhanced antiviral effects against FMDV by combination treatment with Ad-porcine IFN-␣␥ and Ad-3siRNA to overcome the mechanisms of resistance of FMDV in swine.

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“…The adenoviral vectors expressing shRNA have been successfully used in vivo against several viral infections. After pre-treatment of guinea pigs and swine with adenoviral vectors expressing shRNA against foot and mouth disease virus (FMDV), and challenging 24 hours later with the target virus, Chen and colleagues could protect the animals from major clinical manifestation of the disease (Chen et al 2006). Similarly adenoviral vectors have also been used to protect mice against porcine circovirus type 2 (Feng et al 2008).…”

Section: Adenoviral Vectorsmentioning

confidence: 99%

Rna Interference as Antiviral Therapy: Dream or Reality?

Nizamani¹,

Holz²,

Keita³

et al. 2013

VR&R

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Soon aft er discovery of RNA interference (RNAi), its potential as eff ective antiviral therapy was recognized. Since then RNAi has been variously exploited for antiviral purposes which could eff ectively block viral replication in vitro. For in vivo use, however, delivery issue, toxicity, RNAi suppression and viral escape are still major hurdles. Here, we provide an overview of the RNAi strategy and review the approaches that have been developed to surpass the obstacles and to achieve targeted gene silencing for antiviral and other therapies.

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Adenovirus-Mediated RNA Interference against Foot-and-Mouth Disease Virus Infection both In Vitro and In Vivo

Abstract: Foot-and-mouth disease virus (FMDV) infection is responsible for the heavy economic losses in stockbreed-

Cited by 87 publications

References 36 publications

Protective Role of Estrogen-induced miRNA-29 Expression in Carbon Tetrachloride-induced Mouse Liver Injury

Protective Role of Estrogen-induced miRNA-29 Expression in Carbon Tetrachloride-induced Mouse Liver Injury

Robust Protection against Highly Virulent Foot-and-Mouth Disease Virus in Swine by Combination Treatment with Recombinant Adenoviruses Expressing Porcine Alpha and Gamma Interferons and Multiple Small Interfering RNAs

Rna Interference as Antiviral Therapy: Dream or Reality?

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