Adenovirus-mediated gene transfer into monocyte-derived macrophages of patients with X-linked chronic granulomatous disease: ex vivo correction of deficient respiratory burst

Schneider, SD; Rusconi, Sandro; Seger, R; Hossle, Johann P.

doi:10.1038/sj.gt.3300432

Cited by 37 publications

(18 citation statements)

References 10 publications

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“…3a). As reported by others (15,23), we found that non-transgenic macrophages are relatively susceptible to Ad infection and a significant number of cells were GFP positive. However, the number of transgenic GFP-positive macrophages was at least twice the number of GFP-positive control cells (data not shown).…”

Section: Efficient In Vitro Adenovirus Infection Of Cells Of the Hemasupporting

confidence: 65%

A mouse model for adenovirus gene delivery

Tallone

Malin

Samuelsson

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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The cellular attachment receptor for adenovirus (Ad), Coxsackie adenovirus receptor (CAR), required for delivery of Ad into primary cells, is not present on all cell types, thus restricting Ad-gene delivery systems. To circumvent this constrain, a transgenic mouse has been generated that expresses a truncated human CAR in all tissues analyzed. These mice allowed efficient in vitro infections at low multiplicities into lymphoid, myeloid, and endothelial cells. Furthermore, in vivo administration of Ad-vectors results in infection of macrophages, lymphocytes, and endothelial cells. In addition, tail vein injection resulted in targeting of virus into previously inaccessible areas, such as the lung and the capillaries of the brain. The CAR transgenic mice will be useful for rapid functional genomic analysis in vivo, for testing the efficacy of gene therapy procedures or as a source of easily transducible cells.

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Section: Efficient In Vitro Adenovirus Infection Of Cells Of the Hemasupporting

confidence: 65%

A mouse model for adenovirus gene delivery

Tallone

Malin

Samuelsson

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

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“…3 Several strategies have been developed to transfer genes directly into macrophages but most of them use viral vectors. 4 Despite the high transfection efficiency of viral vectors, safety concerns have been raised in clinical trials regarding their potential toxicity. 5 The use of nonviral vectors is attractive for in vivo gene delivery because it is simpler than using viral systems and lacks some of the risks inherent in the latter.…”

Section: Introductionmentioning

confidence: 99%

Mannose receptor-mediated gene transfer into macrophages using novel mannosylated cationic liposomes

et al. 2000

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“…Cells were plated in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10% fetal bovine serum, L-glutamine (Life Technologies), penicillin/streptomycin, and 25 mM KCl at a density of 3 ϫ 10 5 /cm 2 (2.5 ϫ 10 6 /35-mm dish). CPs2 Cells-A PrP expression vector containing murine PrP driven by the CMV promoter was constructed by inserting the murine "halfgenomic" PrP sequence as a blunted BamHI-SalI fragment excised from the plasmid pPrP-5ЈHG SalI (19) into the SalI-BglII restricted and blunted pAd-CMV-lacZ vector (40). The human retina cells 911 (41) were co-transfected with 3 g of pAd-CMV-PrP HG Sal DNA and 0.6 g of the plasmid pSV2neo carrying a neomycin resistance gene (42).…”

Section: Primary Neuron Cultures-cultures Enriched In Granule Neuronsmentioning

confidence: 99%

Similar Turnover and Shedding of the Cellular Prion Protein in Primary Lymphoid and Neuronal Cells

Parizek¹,

Roeckl²,

Weber³

et al. 2001

Journal of Biological Chemistry

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The cellular prion protein (PrP C ) is essential for pathogenesis and transmission of prion diseases. Although prion replication in the brain is accompanied by neurodegeneration, prions multiply efficiently in the lymphoreticular system without any detectable pathology. We have used pulse-chase metabolic radiolabeling experiments to investigate the turnover and processing of PrP C in primary cell cultures derived from lymphoid and nervous tissues. Similar kinetics of PrP C degradation were observed in these tissues. This indicates that the differences between these two organs with respect to their capacity to replicate prions is not due to differences in the turnover of PrP C . Substantial amounts of a soluble form of PrP that lacks the glycolipid anchor appeared in the medium of splenocytes and cerebellar granule cells. Soluble PrP was detected in murine and human serum, suggesting that it might be of physiological relevance.

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Adenovirus-mediated gene transfer into monocyte-derived macrophages of patients with X-linked chronic granulomatous disease: ex vivo correction of deficient respiratory burst

Cited by 37 publications

References 10 publications

A mouse model for adenovirus gene delivery

A mouse model for adenovirus gene delivery

Mannose receptor-mediated gene transfer into macrophages using novel mannosylated cationic liposomes

Similar Turnover and Shedding of the Cellular Prion Protein in Primary Lymphoid and Neuronal Cells

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