Adenovirus E1A protein activation of an integrated viral gene.

Curtois, G.; Berk, Arnold

doi:10.1002/j.1460-2075.1984.tb01943.x

Cited by 27 publications

(8 citation statements)

References 28 publications

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“…The relative efficiency with which the ElA 13S and 12S products induce early viral gene transcription appears to vary greatly, depending on the experimental system or early gene analyzed. In several studies it has been concluded that the 12S mRNA product lacks transcription-inducing activity (4,11,18,37,51). In other cases the ElA 12S mRNA product was shown to induce adenovirus early gene transcription, but at levels somewhat reduced compared with those induced by the 13S mRNA product (10,40,58).…”

Section: Discussionmentioning

confidence: 99%

E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.

Ferguson¹,

Krippl²,

Andrisani³

et al. 1985

Mol. Cell. Biol.

155

101

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We previously purified and characterized functionally the Escherichia coli-expressed product of the human subgroup C adenovirus E1A 13S mRNA (B. Ferguson, N. Jones, J. Richter, and M. Rosenberg, Science 224:1343-1346, 1984; B. Krippl, B. Ferguson, M. Rosenberg, and H. Westphal, Proc. Natl. Acad. Sci. USA 81:6988-6992, 1984). We have now expressed in E. coli and purified the protein product encoded by the human subgroup C adenovirus E1A 12S mRNA and have compared the functional properties of this protein with those of the E1A 13S mRNA product. Using microinjection techniques to introduce these proteins into mammalian cells, we found that the E1A 12S mRNA product, like the 13S mRNA product, localized rapidly to the cell nucleus and induced adenovirus gene expression. Although both E1A gene products localized to the nucleus and stimulated adenovirus gene transcription, these proteins did not directly bind to DNA under conditions in which a known DNA-binding protein, the human c-myc gene product, bound DNA efficiently. Thus, the E1A and myc gene products, which have been related both structurally and functionally, exhibit distinctly different biochemical properties.

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Section: Discussionmentioning

confidence: 99%

E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.

Ferguson¹,

Krippl²,

Andrisani³

et al. 1985

Mol. Cell. Biol.

155

101

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“…The EIA protein can activate expression of endogenous cellular genes (Stein and Ziff, 1984;Rosenthal et al, 1985) and transferred viral genes (Curtois and Berk, 1984). The EIA protein can also have a negative regulatory effect by repressing enhancerinduced stimulation of transcription (Borelli et al, 1984;Velcich and Ziff, 1985).…”

Section: Possible Influence Of Eia Enhancer On Expressionmentioning

confidence: 99%

Stable gene transfer and tissue-specific expression of a human globin gene using adenoviral vectors.

Karlsson¹,

Doren²,

Schweiger³

et al. 1986

The EMBO Journal

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Helper‐free double recombinant adenoviruses containing a genomic human globin gene and the neomycin resistance gene (neoR) have been constructed. The inserted globin and neoR genes are stable and transcription of two human globin genes (beta and a hybrid gamma‐beta gene) is correctly initiated at the respective globin promoter during lytic infection in 293 cells. The neoR gene driven by the SV40 early promoter confers G418 resistance to human fibroblasts and K562 human erythro‐leukemia cells transformed with these viruses. Most neoR clones contain the entire recombinant viral genome, including the inserted globin gene, integrated into their chromosomes. Normally, K562 cells express their gamma but not their beta globin genes. The transferred human beta globin gene was not expressed in either K562 cells or fibroblasts. However, the hybrid gamma‐beta globin gene was expressed in all K562 clones that contained the gene whereas gamma‐beta mRNA was barely detectable in the fibroblasts. This demonstrates tissue‐specific expression of the adenovirus‐transferred globin gene. Furthermore, the two transferred genes, globin and neoR, which are situated more than 20 kb apart in the viral genome appear to be independently regulated.

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“…Therefore, the ability of HSV to activate transcription from viral, but not cellular, genes in the host genome apparently contrasts with the general activation of both viral and cellular promoters resident in a plasmid. The activation of integrated viral genes in adenovirus-transformed cells during subsequent adenovirus infection has been cited as a demonstration of specificity of IE gene product activation, and that this specificity is absent, or masked, during short-term transfection assays (Curtois and Berk, 1984). However, it is possible that integration of foreign genes into the cellular genome during biochemical transformation occurs at locations which are particularly accessible to IE gene product activation.…”

Section: Introductionmentioning

confidence: 99%

Activation of cellular promoters during herpes virus infection of biochemically transformed cells.

Rd¹

1985

The EMBO Journal

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At least two of the immediate‐early (IE) products of herpes simplex virus‐1 (HSV‐1) are responsible for the activation of transcription from viral early promoters. This process appears not to be promoter specific since several unrelated viral and cellular plasmid‐borne promoters can also be activated in short‐term transfection assays. This paper describes experiments that show that cellular promoters integrated into the host genome can also be activated during viral infection, and that this process is brought about by IE gene products. Biochemically transformed cell lines were isolated following transfection of plasmids containing the human epsilon‐globin promoter linked to the herpes thymidine kinase coding region (as selectable marker), and an unselected rabbit beta‐globin gene. Infection of some, but not all, such cell lines with HSV‐1 resulted in a rapid and considerable stimulation of the integrated epsilon‐ and beta‐globin promoters. Both promoters could also be activated (albeit less efficiently) during pseudorabies virus infection, and after introduction by transfection of plasmids containing HSV IE genes. The implications of these results for viral‐host interactions and the mechanism of viral‐induced promoter activation are discussed.

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Adenovirus E1A protein activation of an integrated viral gene.

Cited by 27 publications

References 28 publications

E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.

E1A 13S and 12S mRNA products made in Escherichia coli both function as nucleus-localized transcription activators but do not directly bind DNA.

Stable gene transfer and tissue-specific expression of a human globin gene using adenoviral vectors.

Activation of cellular promoters during herpes virus infection of biochemically transformed cells.

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