Adenovirus Antigens--A Study of their Properties and Sequential Development in Infection

Abstract

Antisera were prepared which reacted specifically with different antigens in the capsid of adenovirus type 5, viz. hexons, pentons, fibres and penton bases. A rabbit antiserum was obtained which reacted with another antigen (designated P) formed during the early stages of the infectious process. This antigen is similar to the T antigen associated with infection by the oncogenic adenoviruses. Using the specific antisera and the P antiserum a study was made of the properties and sequence of development of the antigens in single-step infection. The time of appearance of the antigens was a function of the added multiplicity of infection. The P antigen was detected early in infection and was followed 4 hr later by the fibres and hexons and a further 2 hr later by the penton bases. The appearance of the penton bases was accompanied by an increase in infectivity. Haemagglutination and the ability of the antigens to inhibit cell spreading were measured and it is concluded that relatively late in infection the capsid antigens are no longer incorporated into virus particles and can be detected as free antigens.

Monoclonal Antibodies against Adenovirus Type 5: Preparation and Preliminary Characterization

“…These tests performed with extracts of infected cells have also been described in previous publications (Russell et al, 1967(Russell et al, , 1972.…”

“…It will be of interest to ascertain if this hybridoma can inhibit the cell detaching activity associated with penton base (Russell et al, 1967).…”

“…Preparation and purification of human adenovirus type 5 (Ad 75) has been described previously (Russell et al, 1967;Winters & Russell, 1971). Virus was produced in KB cells grown in suspension and labelling of infected cells was carried out in HeLa cell monolayers as outlined previously (Russell & Blair, 1977).…”

“…This was carried out using rat and monkey erythrocytes as described previously (Russell et al, 1967).…”

Nucleic Acid-binding Properties of Adenovirus Structural Polypeptides

“…Preparation and purification of human adenovirus type 5 (Ad5; strain Ad75) has been described in detail previously (Russell et al, 1967;Winters & Russell, 1971). Virus was produced in KB or HeLa The terminology of the polypeptides is that suggested by Anderson et al (1973).…”