Adenoviromics: Mining the Human Adenovirus Species D Genome

Ismail, Ashrafali Mohamed; Lee, Ji Sun; Lee, Yoo‐Hyun; Singh, Gurdeep; Dyer, David W.; Seto, Donald; Chodosh, James; Rajaiya, Jaya

doi:10.3389/fmicb.2018.02178

Cited by 41 publications

(28 citation statements)

References 159 publications

(213 reference statements)

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“…The hypothesis is that the homologous recombination between two or among multi-types occurs in the gastrointestinal tract, where numerous diverse microorganisms live. There are hints that the bacterial Rec enzymes from E. coli enabled the recombination in immunocompromised individuals [ 28 , 29 ]. Multiple alignments of the current four species D types also revealed highly conserved sequences ( Figure 3 a and Figure 4 a).…”

Section: Discussionmentioning

confidence: 99%

High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75

Zhang

Mese

Schellhorn

et al. 2020

IJMS

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Recently an increasing number of new adenovirus types associated with type-dependent pathogenicity have been identified. However, identification of these clinical isolates represents the very first step to characterize novel pathogens. For deeper analyses, these adenoviruses need to be further characterized in basic virology experiments or they could be applied in translational research. To achieve this goal, it is essential to get genetic access and to enable genetic modification of these novel adenovirus genomes (deletion, insertion, and mutation). Here we demonstrate a high-throughput approach to get genetic access to new adenoviruses via homologous recombination. We first defined the cloning conditions regarding homology arm-length and input adenoviral genome amounts. Then we cloned four naturally occurring adenoviruses (Ad70, Ad73, Ad74, and Ad75) into easy-to-manipulate plasmids and genetically modified them by reporter gene insertion. Three recombinant adenoviruses (Ad70, Ad73, and Ad74) containing a reporter cassette were successfully reconstituted. These novel reporter-labeled adenoviruses were further characterized using the inserted luciferase reporter with respect to receptor usage, presence of anti-adenovirus antibodies, and tropism in vitro. The identified receptor usage, the relatively low prevalence of anti-adenovirus antibodies, and the various cancer cell line transduction pattern are important features of these new pathogens providing essential information for their therapeutic application.

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Section: Discussionmentioning

confidence: 99%

High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75

Zhang

Mese

Schellhorn

et al. 2020

IJMS

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“…HAdV-C seemingly codiverged with gorillas, chimpanzees, bonobos and humans, whereas HAdV-D viruses infect only humans (Hoppe et al 2015). The long-term coevolution of HAdV-D and humans is supported by the high variety of types within the species (Ismail et al 2018b;Kaján et al 2018), and their usually facultative pathogen nature.…”

Section: Adenovirusesmentioning

confidence: 99%

Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses

et al. 2019

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Viruses have been infecting their host cells since the dawn of life, and this extremely long-term coevolution gave rise to some surprising consequences for the entire tree of life. It is hypothesised that viruses might have contributed to the formation of the first cellular life form, or that even the eukaryotic cell nucleus originates from an infection by a coated virus. The continuous struggle between viruses and their hosts to maintain at least a constant fitness level led to the development of an unceasing arms race, where weapons are often shuttled between the participants. In this literature review we try to give a short insight into some general consequences or traits of virus-host coevolution, and after this we zoom in to the viral clades of adenoviruses, herpesviruses, nucleo-cytoplasmic large DNA viruses, polyomaviruses and, finally, circoviruses.

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“…The HAdVs are non‐enveloped viruses belonging to the Mastadenovirus genus of the Adenoviridae family. Based on the biological and genetic features, seven species (A‐G) of HAdV have been documented, which further divided into 90 types . Among seven species, HAdV‐F representing AdV‐ 40 and 41, and HAdV‐A representing AdV‐ 12, 18, and 31, are named enteric AdVs (EAdVs) based on tissue tropism and disease patterns that are associated mainly with the gastrointestinal tract .…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of human adenoviruses in hospitalized children <5 years old with acute gastroenteritis in Tehran, Iran

Arashkia

Bahrami

Farsi

et al. 2019

Journal of Medical Virology

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Human adenoviruses (HAdVs), especially AdV-40 and 41, are common causes of nonbacterial sporadic and outbreak gastroenteritis in children. The present study aimed to describe the frequency and genetic analysis of HAdVs in hospitalized children <5 years old with acute gastroenteritis. A total of 376 stool samples obtained from June 2015 to December 2017 were investigated for the presence of HAdVs by polymerase chain reaction. The HAdV DNA was detected in 16 (4.3%) out of 376 stool samples. Based on the hexon hypervariable region (HVR), B, C, and F HADV species including five types HAdV-1, 2, 3, 6, and 41 were identified, among which enteric AdV species F (EAdV-41) was the most dominant. Moreover, our findingsshowed the presence of genomic type cluster 1 (GTC1) pattern in Iranian type 41 strains, which was closely similar to the D1 prototype strain (Tak) and D28. In this regard, a recombination was found in AdV-41 strains presenting the hexon sequence that belonged to GTC1, while fiber sequence clustered with GTC2.

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Adenoviromics: Mining the Human Adenovirus Species D Genome

Cited by 41 publications

References 159 publications

High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75

High-Throughput Cloning and Characterization of Emerging Adenovirus Types 70, 73, 74, and 75

Virus–Host Coevolution with a Focus on Animal and Human DNA Viruses

Molecular analysis of human adenoviruses in hospitalized children <5 years old with acute gastroenteritis in Tehran, Iran

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