Adenosine triphosphate-selective fluorescent turn-on response of a novel thiazole orange derivative via their cooperative co-assembly

Deng, Tuo; Chen, Jianhui; Yu, Hui; Yang, Pin; Jian, Yang; Li, Gang; Zhou, Xue; Shen, Hongyan; Gui, Jianzhou

doi:10.1016/j.snb.2014.12.008

Cited by 19 publications

(8 citation statements)

References 25 publications

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“…On its own, TO can be chemically modified to exhibit increased fluorescence in response to small molecules or ions. For example, a study of a TO-derivative appended by two 17-atom-long aliphatic chains found that it exhibited selective increased fluorescence in the presence of ATP, relative to AMP, ADP, and other nucleoside triphosphates [ 127 ].…”

Section: To In the Sensing Of Small Molecules And Ionsmentioning

confidence: 99%

Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions

Suss,

Motiei,

Margulies

2021

Molecules

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Fluorescent sensing of biomolecules has served as a revolutionary tool for studying and better understanding various biological systems. Therefore, it has become increasingly important to identify fluorescent building blocks that can be easily converted into sensing probes, which can detect specific targets with increasing sensitivity and accuracy. Over the past 30 years, thiazole orange (TO) has garnered great attention due to its low fluorescence background signal and remarkable ‘turn-on’ fluorescence response, being controlled only by its intramolecular torsional movement. These features have led to the development of numerous molecular probes that apply TO in order to sense a variety of biomolecules and metal ions. Here, we highlight the tremendous progress made in the field of TO-based sensors and demonstrate the different strategies that have enabled TO to evolve into a versatile dye for monitoring a collection of biomolecules.

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Section: To In the Sensing Of Small Molecules And Ionsmentioning

confidence: 99%

Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions

Suss,

Motiei,

Margulies

2021

Molecules

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“…As an asymmetric cyanine dye, TO can freely penetrate into all cells [ 30 ], but it does not produce fluorescence in aqueous solution. Once TO integrates with nucleic acids, it generates a remarkable fluorescence enhancement on account of the restriction of intramolecular rotations between benzothiazole and quinoline heterocycles [ 31 , 32 ]. Accordingly, TO is often used as a fluorescent probe for nucleic acid detection [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

A Combination of Novel Nucleic Acid Cross-Linking Dye and Recombinase-Aided Amplification for the Rapid Detection of Viable Salmonella in Milk

Feng

Zhou²,

Gan³

et al. 2022

Foods

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Salmonella, as an important foodborne pathogen, can cause various diseases, such as severe enteritis. In recent years, various types of nucleicacid-intercalating dyes have been utilized to detect viable Salmonella. However, in principle, the performance of existing nucleic acid dyes is limited because they depend on the integrity of cell membrane. Herein, based on the metabolic activity of bacteria, a novel DNA dye called thiazole orange monoazide (TOMA) was introduced to block the DNA from dead bacteria. Recombinase-aided amplification (RAA) was then performed to detect viable Salmonella in samples. In this study, the permeability of TOMA to the cell membrane of Salmonella was evaluated via confocal laser scanning microscopy and fluorescence emission spectrometry. The limit of detection (LOD) of the TOMA–RAA method was 2.0 × 104 CFU/mL in pure culture. The feasibility of the TOMA–RAA method in detecting Salmonella was assessed in spiked milk. The LOD for Salmonella was 3.5 × 102 CFU/mL after 3 h of enrichment and 3.5 × 100 CFU/mL after 5 h of enrichment. The proposed TOMA–RAA assay has great potential to be applied to accurately detect and monitor foodborne pathogens in milk and its byproducts.

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“…Ad ifferent sensing principle wasr eportedb yG ui and coworkers, who used thiazoleo range (TO) derivative 15 as as elective probe for ATP. [16] It is well knownt hat TO has as ignificant fluorescencee nhancement when the torsion motion along the doubleb ond is restricted as ar esult of complex formation with DNA. Indeed, addition of nucleotides to the receptors in a1 0mm HEPES buffer (pH 7.36) resultedi nafluorescent enhancement.…”

Section: Functional Dyes For Nucleotide Sensingmentioning

confidence: 99%

Molecular Receptors for Recognition and Sensing of Nucleotides

Agafontsev

Ravi

Shumilova

et al. 2018

Chemistry A European J

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Nucleotides are constituents of nucleic acids and they have a variety of functions in cellular metabolism. Synthetic receptors and sensors are required to reveal the role of nucleotides in living organisms and mechanisms of signal transduction events. In recent years, a large number of nucleotide‐selective synthetic receptors have been devised, which utilize different molecular designs and sensing mechanisms. This Minireview presents recent progress in the design of synthetic molecular receptors for selective recognition of nucleotides in aqueous solution. The binding properties of receptors and the origins of their selectivity for a particular nucleotide are discussed.

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Adenosine triphosphate-selective fluorescent turn-on response of a novel thiazole orange derivative via their cooperative co-assembly

Cited by 19 publications

References 25 publications

Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions

Broad Applications of Thiazole Orange in Fluorescent Sensing of Biomolecules and Ions

A Combination of Novel Nucleic Acid Cross-Linking Dye and Recombinase-Aided Amplification for the Rapid Detection of Viable Salmonella in Milk

Molecular Receptors for Recognition and Sensing of Nucleotides

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