Adenosine monophosphate-activated protein kinase modulation by berberine attenuates mitochondrial deficits and redox imbalance in experimental diabetic neuropathy

Yerra, Veera Ganesh; Kalvala, Anil Kumar; Sherkhane, Bhoomika; Areti, Aparna; Kumar, Ashutosh

doi:10.1016/j.neuropharm.2017.12.029

Cited by 53 publications

(48 citation statements)

References 63 publications

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“…Cells were incubated for 48 hours with the corresponding treatments and loaded with 10 µmol/L DCFH‐DA for another 1 hour at 37°C. Cells were collected and analyzed by FACS according to the manufacturer's instructions . Intracellular DCF fluorescence was detected at wavelengths of 488 nm for excitation and 525 nm for emission using fluorescence microplate reader (Thermo Fischer Scientific) and quantified.…”

Section: Methodsmentioning

confidence: 99%

The therapeutic role of baicalein in combating experimental periodontitis with diabetes via Nrf2 antioxidant signaling pathway

Zhao

et al. 2019

J of Periodontal Research

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Background and objective:Oxidative stress has been suggested as an important pathogenic factor contributing to chronic periodontitis with diabetes mellitus (CPDM). Previous studies have revealed the potential therapeutic properties of baicalein (BCI) in oxidative stress-related diseases; however, the antioxidant effects of BCI on therapy for individual with CPDM remain largely unexplored. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays a critical role in cellular defence against oxidative stress. In this study, we aim to determine whether BCI prevents diabetesrelated periodontal tissue destruction by regulating Nrf2 signaling pathway. Material and methods: Human gingival epithelial cells (hGECs) were challenged with high glucose (HG, 25 mmol/L) and/or lipopolysaccharide (LPS, 20 µg/mL). Reactive oxygen species (ROS) were detected by fluorescence-activated cell sorting. The changes of antioxidant-related genes, including Nrf2, catalase (Cat), glutamatecysteine ligase catalytic subunit (Gclc), superoxide dismutase 1 (Sod1), and superoxide dismutase 2 (Sod2), were quantified by real-time PCR. The localization of phospho-Nrf2 (pNrf2, S40) in the nucleus was detected by immunofluorescence staining and laser scanning confocal microscope (LSCM). PNrf2 and total form of Nrf2 were determined using western blot. The above indicators together with mitochondrial membrane potential (MMP) were further investigated in hGECs pre-treated with different concentrations of BCI (0.01, 0.1, or 0.5 µg/mL) before stimulated with HG plus LPS (GP). Finally, the role of BCI in activating Nrf2 signaling pathway and relieving the alveolar bone absorption was examined in the CPDM model of Sprague Dawley rats.CPDM rats were oral gavaged with BCI (50, 100, or 200 mg/kg daily). The pNrf2 was detected by immunohistochemistry, and the alveolar bone absorption was examined by microcomputed tomography. Results:Our results showed that ROS were significantly increased in both groups of HG and LPS, with the strongest generation in the GP group. In terms of ROSrelated gene expression, we found that the mRNA levels of Nrf2, Cat, Gclc, Sod1, and Sod2 were significantly decreased in HG and LPS groups. In consistent with the strongest induction of ROS in GP group, the gene expression in GP group was further

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Section: Methodsmentioning

confidence: 99%

The therapeutic role of baicalein in combating experimental periodontitis with diabetes via Nrf2 antioxidant signaling pathway

Zhao

et al. 2019

J of Periodontal Research

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“…In addition, berberine reduced hyperphosphorylation of tau and APP [29,39], possibly through inactivation of GSK3β via Akt signaling [29]. On the other hand, growing evidence suggests that berberine prevents dementia associated with diabetes [43][44][45][46][47][48][49][50][51][52][53][54][55][61][62][63] (Table 2). Berberine inhibited apoptosis in the hippocampus and cerebral cortex, suppressed anxiety and restored recognition memory in the sporadic AD model induced by intracerebroventricular injection of streptozotocin (STZ), possibly via reduction of AChE activity [44,51].…”

Section: Ischemia-reperfusion Injury In T2dmmentioning

confidence: 99%

“…Of note, berberine supplementation restored glucose transporter levels and insulin signaling in the brain [50], suggesting that modulation of glucose metabolism in the brain may underlie the neuroprotective effect of berberine. Moreover, berberine activated 5'-adenosine monophosphate-activated protein kinase (AMPK), a key regulator of cellular energy homeostasis, restored mitochondrial membrane potential in neurons under high glucose (HG) stress [63] and protected neurons from glucose-induced oxidative stress in vitro [37,47], suggesting that berberine could alleviate neuronal damages under hyperglycemia.…”

Section: Ischemia-reperfusion Injury In T2dmmentioning

confidence: 99%

Berberine for prevention of dementia associated with diabetes and its comorbidities: A systematic review

Shinjyo

Parkinson

Bell

et al. 2020

Journal of Integrative Medicine

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…[29]. Recent studies have discovered BBR's effect on the other signaling pathways, such as PI3K-Akt, p38MAPK and AMPK signaling etc., in brain disorder [30][31][32], showing the complex mechanism of BBR neuroprotective effect. The anti-depression and anti-anxiety effects are newly identified pharmacological activities of BBR against neuropsychiatric disorders [33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of the neurons in mice with depressive-like behavior induced by corticosterone and the regulation of berberine: pivotal sites of oxidative phosphorylation

et al. 2019

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Chronic corticosterone (CORT) stress is an anxiety and depression inducing factor that involves the dysfunction of glucocorticoid receptor (GR), brain-derived neurotrophic factor (BDNF), and neuronal plasticity. However, the regulation of proteomic profiles in neurons suffering CORT stress is remaining elusive. Thus, the proteomic profiles of mouse neuronal C17.2 stem cells were comprehensively investigated by TMT (tandem mass tag)-labeling quantitative proteomics. The quantitative proteomics conjugated gene ontology analysis revealed the inhibitory effect of CORT on the expression of mitochondrial oxidative phosphorylation-related proteins, which can be antagonized by berberine (BBR) treatment. In addition, animal studies showed that changes in mitochondria by CORT can affect neuropsychiatric activities and disturb the physiological functions of neurons via disordering mitochondrial oxidative phosphorylation. Thus, the mitochondrial energy metabolism can be considered as one of the major mechanism underlying CORT-mediated depression. Since CORT is important for depression after traumatic stress disorder, our study will shed light on the prevention and treatment of depression as well as posttraumatic stress disorder (PTSD).

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Adenosine monophosphate-activated protein kinase modulation by berberine attenuates mitochondrial deficits and redox imbalance in experimental diabetic neuropathy

Cited by 53 publications

References 63 publications

The therapeutic role of baicalein in combating experimental periodontitis with diabetes via Nrf2 antioxidant signaling pathway

The therapeutic role of baicalein in combating experimental periodontitis with diabetes via Nrf2 antioxidant signaling pathway

Berberine for prevention of dementia associated with diabetes and its comorbidities: A systematic review

Proteomic profiling of the neurons in mice with depressive-like behavior induced by corticosterone and the regulation of berberine: pivotal sites of oxidative phosphorylation

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