Adenosine deaminase activity in peripheral blood cells of patients with haematological malignancies

Meier, John R.; Colemán, Mary Sue; Hutton, John J.

doi:10.1038/bjc.1976.45

Cited by 81 publications

(25 citation statements)

References 8 publications

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“…ADA activity has been found to be subnormal in the lymphocytes of patients with chronic lymphocytic leukemia (9, 10, 11) a disease characterized by immunologic impairment and an abnormal proliferation of B cells (12). We previously demonstrated that the ADA activity of acute leukemia lymphoblasts is greatly increased in comparison with normal lymphocyte levels (3), and this has subsequently been confirmed by other studies (4,11). The considerable variation in absolute ADA activity among ALL patients noted previously, and the known immunological heterogeneity of ALL, prompted us to study leukemic lymphoblasts to determine whether a correlation exists between ADA activity and immunological subclasses of ALL.…”

Section: Introductionsupporting

confidence: 52%

Correlation of adenosine deaminase activity with cell surface markers in acute lymphoblastic leukemia.

Smyth¹,

Poplack²,

Holiman³

et al. 1978

J. Clin. Invest.

126

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Section: Introductionsupporting

confidence: 52%

Correlation of adenosine deaminase activity with cell surface markers in acute lymphoblastic leukemia.

Smyth¹,

Poplack²,

Holiman³

et al. 1978

J. Clin. Invest.

126

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“…However, the marked increases of ADA activity in rapidly growing hepatomas (Fig. 3) anid in acute leukaemia cells (Smyth and Harrap, 1975;Meier et al, 1976) suggest that this enzyme may correlate with the process of malignant progression. A lack of correlation of ADA activity with cell proliferation per se seems to be indicated by the unchanged values in regenerating liver and neonatal liver.…”

Section: D)1scussionmentioning

confidence: 96%

Adenosine deaminase and adenosine kinase in rat hepatomas and kidney tumours

Jackson¹,

Morris²,

Weber³

1978

Br J Cancer

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Summary.-Adenosine deaminase and adenosine kinase have been measured in rat liver, 12 transplantable hepatomas, regenerating, foetal and neonatal liver, adult and neonatal rat kidney and 2 transplantable kidney tumours. Adenosine deaminase activity, relative to the normal liver value, was elevated 2-4-fold in hepatomas of rapid growth rate, was in the normal range in more slowly growing hepatomas and in regenerating liver, and was low in foetal and neonatal liver. Adenosine kinase activity was decreased, relative to rat liver values, in all the hepatomas; activity of this enzyme gave a negative correlation with tumour growth rate. Kinetic properties of the two enzymes were examined in partially purified preparations. Adenosine deaminases from both liver and rapidly growing hepatoma 3924A were subject to weak product inhibition by inosine. Adenosine kinase from liver and hepatoma 3924A was inhibited by the reaction products ADP and AMP, and the enzyme was also subject to excess substrate inhibition by concentrations of ATP in excess of 1 mM. In rat hepatoma cell lines growing in culture, the toxicity of adenosine correlated inversely with the ratio of adenosine deaminase activity to adenosine kinase activity. Chromatographic measurements showed that hepatoma cells incorporated less extracellular adenosine into their adenine nucleotide pools than did isolated liver cells. These results indicate that increased adenosine deaminase activity and decreased adenosine kinase activity may confer a selective advantage upon the cancer cell.

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“…Decreased levels of ADA have been re-ported in lymphocytes of chronic lymphocy tic leukaemia (CLL) [12,15,18]. ADA ac tivity is markedly increased in blast cells of patients with acute lymphocytic leukaemia [12,16] and acute myelogenous leukaemia (AML) [12] but normal to moderately in creased in both leukaemias in remission as long as they were treated with cytostatic drugs [19].…”

Section: Introductionmentioning

confidence: 99%

“…ADA ac tivity is markedly increased in blast cells of patients with acute lymphocytic leukaemia [12,16] and acute myelogenous leukaemia (AML) [12] but normal to moderately in creased in both leukaemias in remission as long as they were treated with cytostatic drugs [19]. In contrast to the severe com bined immunodeficiency, increased ADA activity was found in patients treated with immunosuppressive drugs [19].…”

Section: Introductionmentioning

confidence: 99%

Adenosine Deaminase Activity in Plasma and Blood Cells of Patients with Haematological and Autoimmune Diseases

Storch

Krüger

Rotzsch³

1981

Acta Haematol

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Adenosine deaminase (ADA) has been assayed in plasma, erythrocytes, and lymphocytes from 29 patients with haematological and autoimmune diseases. ADA activity was uniformly low in erythrocytes and lymphocytes from patients with non-Hodgkin lymphoma and multiple myeloma (p < 0.001). High levels of ADA activity was found in plasma, erythrocytes, and lymphocytes from patients with myeloid leukemia (p < 0.001). ADA was high in plasma but low in erythrocytes and lymphocytes from patients with autoimmune diseases treated with immunosuppressive drugs (p < 0.05). 4 adults with congenital immunodeficiency showed decreased ADA activity. In the control group of normal blood donors we found a 34-year-old female with low ADA activity in plasma, erythrocytes, and lymphocytes without any immunological abnormalities. This is the 3rd case of a healthy individual deficient for ADA. 1 patient with Osier’s disease and high ADA activity in erythrocytes showed the importance of the purine salvage enzyme not only in lymphocytes.

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Adenosine deaminase activity in peripheral blood cells of patients with haematological malignancies

Cited by 81 publications

References 8 publications

Correlation of adenosine deaminase activity with cell surface markers in acute lymphoblastic leukemia.

Correlation of adenosine deaminase activity with cell surface markers in acute lymphoblastic leukemia.

Adenosine deaminase and adenosine kinase in rat hepatomas and kidney tumours

Adenosine Deaminase Activity in Plasma and Blood Cells of Patients with Haematological and Autoimmune Diseases

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