Adenosine 5′-monophosphate decreases citrate exudation and aluminium resistance in Tamba black soybean by inhibiting the interaction between 14-3-3 proteins and plasma membrane H+-ATPase

Min, Yong; Guo, Chenglin; Xiu-ling, Zhao; Wang, Lin; Yu, Yang; Chen, Limei

doi:10.1007/s10725-017-0339-3

Cited by 8 publications

(6 citation statements)

References 26 publications

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“…Citrate, a tricarboxylic acid, supports phosphorus solubilization, enhances phosphorus acquisition through root exudation, acidifies the rhizosphere, affects nutrient interactions, and participates in oxidative stress defense (Li et al, 2023; Khan et al, 2023; Roychowdhury et al, 2023). Adenosine monophosphate (AMP) serves as an energy storage molecule, a signaling molecule triggering stress responses, and contributes to metabolic adjustments, promoting adaptability to low phosphorus stress (Min et al, 2018). 4‐Pyridoxate, a vitamin B6‐related compound, aids in phosphorus uptake and transport, recycling, stress responses, metabolic adjustments, and antioxidant defense, enhancing adaptability to nutrient limitations (Parra et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Tantriani,

Cheng,

Oikawa

et al. 2023

Physiologia Plantarum

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Phosphorus (P) deficiency alters the root morphological and physiological traits of plants. This study investigates how soybean cultivars with varying low‐P tolerance values respond to different P levels in hydroponic culture by assessing alterations in root length, acid phosphatase activity, organic acid exudation, and metabolites in root exudates. Three low‐P‐tolerant cultivars (‘Maetsue,’ ‘Kurotome,’ and ‘Fukuyutaka’) and three low‐P‐sensitive cultivars (‘Ihhon,’ ‘Chizuka,’ and ‘Komuta’) were grown under 0 (P0) and 258 μM P (P8) for 7 and 14 days after transplantation (DAT). Low‐P‐tolerant cultivars increased root length by 31% and 119%, which was lower than the 62% and 144% increases in sensitive cultivars under P0 compared to P8 at 7 and 14 DAT, respectively. Acid phosphatase activity in low‐P‐tolerant cultivars exceeded that in sensitive cultivars by 5.2‐fold and 2.0‐fold at 7 and 14 DAT. Root exudates from each cultivar revealed 177 metabolites, with higher organic acid exudation in low‐P‐tolerant than sensitive cultivars under P0. Low‐P‐tolerant cultivars increased concentrations of specific metabolites (oxalate, GABA, quinate, citrate, AMP, 4‐pyridoxate, and CMP), distinguishing them from low‐P‐sensitive cultivars under P0. The top five metabolomic pathways (purine metabolism, arginine and proline metabolism, TCA cycle, glyoxylate and dicarboxylate metabolism, alanine, aspartate, and glutamate metabolism) were more pronounced in low‐P‐tolerant cultivars at 14 DAT. These findings indicate that increasing root length was not an adaptation strategy under P deficiency; instead, tolerant cultivars exhibit enhanced root physiological traits, including increased acid phosphatase activity, organic acid exudation, specific metabolite release, and accelerated metabolic pathways under P deficiency.

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Section: Discussionmentioning

confidence: 99%

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Tantriani,

Cheng,

Oikawa

et al. 2023

Physiologia Plantarum

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“…Root length was measured before and after treatment. RRG analysis was carried out according to the procedures described by Min [13]. The analysis was performed with three replicates with 5 plants per replicate.…”

Section: Al 3+ Treatment and Measurement Of The Relative Root Growth mentioning

confidence: 99%

“…Our previous research also demonstrated that phosphorylation is responsible for the interaction of H + -ATPase and 14-3-3 protein, which leads to Al 3+ -stimulated citrate exudation in roots of Tamba black soybean (Glycine max cv. Tamba, TBS) [12][13][14]. Additionally, genome-wide association analysis [15], transcriptomics [9,16] and proteomics [17] have allowed the exploration of the mechanisms of Al 3+ resistance globally.…”

Section: Introductionmentioning

confidence: 99%

Quantitative phosphoproteomic analysis provides insights into the aluminum-responsiveness of Tamba black soybean

et al. 2020

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Aluminum (Al 3+) toxicity is one of the most important limitations to agricultural production worldwide. The overall response of plants to Al 3+ stress has been documented, but the contribution of protein phosphorylation to Al 3+ detoxicity and tolerance in plants is unclear. Using a combination of tandem mass tag (TMT) labeling, immobilized metal affinity chromatography (IMAC) enrichment and liquid chromatography-tandem mass spectrometry (LC-MS/MS), Al 3+-induced phosphoproteomic changes in roots of Tamba black soybean (TBS) were investigated in this study. The Data collected in this study are available via ProteomeXchange with the identifier PXD019807. After the Al 3+ treatment, 189 proteins harboring 278 phosphosites were significantly changed (fold change > 1.2 or < 0.83, p < 0.05), with 88 upregulated, 96 downregulated and 5 up-/downregulated. Enrichment and protein interaction analyses revealed that differentially phosphorylated proteins (DPPs) under the Al 3+ treatment were mainly related to G-protein-mediated signaling, transcription and translation, transporters and carbohydrate metabolism. Particularly, DPPs associated with root growth inhibition or citric acid synthesis were identified. The results of this study provide novel insights into the molecular mechanisms of TBS post-translational modifications in response to Al 3+ stress.

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“…Aluminium tolerance mechanisms to Al toxicity in plants can be divided into those that facilitate the exclusion of Al 3+ from root cells (exclusion mechanisms) and those that enable plants to tolerate Al 3+ once it has entered on the root and shoot symplast, characterizing the internal tolerance mechanisms (Ma et al, 2001;Horst et al, 2010;Daspute et al, 2017;Kopittke et al, 2017). Furthermore, the physiological and molecular basis of these mechanisms have been intensively investigated in a myriad of plant species (Min et al, 2018;Arroyave et al, 2018;Silva et al, 2018a, b). Some plants secrete organic acids (especially citrate, malate, and oxalate), which are Al-chelating, and phenols into the soil as a strategy to mitigate Al toxicity in acid soils (Chen and Liao, 2016;Singh et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…At the cellular level, Al detoxification includes the formation of Al complexes with organic acids and sequestration in vacuoles to maintain low levels of free Al in the cytosol (Singh et al, 2017). Besides, few studies have stated that Al stress increases plasma membrane (PM) H + -ATPase activity and citrate secretion and simultaneously enhances the interaction between 14-3-3 proteins and phosphorylated PM H + -ATPase in the root tips of Al-tolerant soybean (Guo et al, 2013;Min et al, 2018). Furthermore, Al sensitivity in some soybean varieties has been attributed to the low level of citrate metabolism and exudation in the roots and the high level of jasmonic acid-mediated defence response in the leaves (Huang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Depicting the physiological and ultrastructural responses of soybean plants to Al stress conditions

Reis

Lisboa

Reis

et al. 2018

Plant Physiology and Biochemistry

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Aluminium (Al) is a toxic element for plants living in soils with acidic pH values, and it causes reductions in the roots and shoots development. High Al concentrations can cause physiological and structural changes, leading to symptoms of toxicity in plant tissue. The aim of this study was to describe the Al toxicity in soybean plants through physiological, nutritional, and ultrastructure analyses. Plants were grown in nutrient solution containing increasing Al concentrations (0; 0.05; 0.1; 1.0, 2.0 and 4.0 mmol L). The Al toxicity in the soybean plants was characterized by nutritional, anatomical, physiological, and biochemical analyses. The carbon dioxide assimilation rates and stomatal conductance were not affected by the Al. However, the capacity for internal carbon use decreased, and the transpiration rate increased, resulting in increased root biomass at the lowest Al concentration in the nutrient solution. The soybean plants exposed to the highest Al concentration exhibited lower root and shoot biomass. The nitrate reductase and urease activities decreased with the increasing Al concentration, indicating that nitrogen metabolism was halted. The superoxide dismutase and peroxidase activities increased with the increasing Al availability in the nutrient solution, and they were higher in the roots, showing their role in Al detoxification. Despite presenting external lesions characterized by a damaged root cap, the root xylem and phloem diameters were not affected by the Al. However, the leaf xylem diameter showed ultrastructural alterations under higher Al concentrations in nutrient solution. These results have contributed to our understanding of several physiological, biochemical and histological mechanisms of Al toxicity in soybean plants.

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Adenosine 5′-monophosphate decreases citrate exudation and aluminium resistance in Tamba black soybean by inhibiting the interaction between 14-3-3 proteins and plasma membrane H+-ATPase

Cited by 8 publications

References 26 publications

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Phosphorus deficiency alters root length, acid phosphatase activity, organic acids, and metabolites in root exudates of soybean cultivars

Quantitative phosphoproteomic analysis provides insights into the aluminum-responsiveness of Tamba black soybean

Depicting the physiological and ultrastructural responses of soybean plants to Al stress conditions

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