Additive manufacturing of tissues and organs

Melchels, Ferry P.W.; Domingos, Marco; Klein, Travis J.; Malda, Jos; Bartolo, Paulo Jorge Da Silva; Hutmacher, Dietmar W.

doi:10.1016/j.progpolymsci.2011.11.007

Cited by 1,040 publications

(707 citation statements)

References 171 publications

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“…Moreover, the ability of SLS to manufacture anatomically shaped scaffolds with designed microstructure made of bioactive and bioresorbable composite materials at high filler loadings allows for fabrication of scaffolds with a high degree of geometric complexity and enables the direct conversion of the digital representation of any object into its physical realisation. The method also enables the development of patient and tissue-specific reconstruction strategies [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Powder bed generation in integrated modelling of additive layer manufacturing of orthopaedic implants

Krzyżanowski

Svyetlichnyy

Stevenson

et al. 2016

Int J Adv Manuf Technol

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This paper presents an original model of powder bed generation developed within the frame of an integrated modelling approach for studying the interaction of physical mechanisms in additive layer manufacturing (ALM) of orthopaedic implants. The model is based on cellular automata (CA) approach and describes the relationship between moving particles of different sizes during deposition on a surface in three dimensions. The surface is defined by the horizontal two-dimensional CA on which particles fall and irreversibly stick to a growing deposit. The model allows for consideration of different restructuring cases when particles are allowed to rotate as often as necessary until achievement of a local minimum position. Changes in the packing density of the powder bed have been investigated numerically depending on technological parameters, such as particle size distribution, deposition rate and sequence of powder deposition. The model has been developed with the aim of merging to the finite element (FE)-based integrated model and is applicable to a different ranges of materials including metals and also non-metals.

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Section: Introductionmentioning

confidence: 99%

Powder bed generation in integrated modelling of additive layer manufacturing of orthopaedic implants

Krzyżanowski

Svyetlichnyy

Stevenson

et al. 2016

Int J Adv Manuf Technol

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“…The direct depth-controlled delivery of picoliter doses over two dimensions opens up new possibilities to study local effects of drug or growth factor delivery, for instance in tissue-engineering applications [12]. Furthermore, combining more powerful lasers with viscous inks [33] could potentially enable our device to generate faster microjets for needle-free injection into stiffer tissues or materials than the soft gelatin susbtrate used in this paper.…”

Section: Resultsmentioning

confidence: 98%

“…3b) over a two-dimensional area with a ± 25 μm depth control as well as a ± 4 μm lateral precision and a 12 ± 4 μm lateral dispersion. This micrometric lateral and axial control over the liquid delivery is comparable to the size of a cell and therefore falls within the range of the optimal printing precision for direct three-dimensional delivery of cells or particles [12]. We thus investigate in the next section the ability of our LIFT system to deliver particles at a pinpoint location in a volume.…”

Section: Depth-controlled Injection For Direct Three-dimensional Liqumentioning

confidence: 93%

“…Improving the lateral resolution of injection would open up possibilities to study the local effect of drug or growth factor delivery, for instance into tissue-engineered constructs for drug screening applications or bio-engineering [12]. Recently, a 30 μm lateral resolution was obtained for the injection of nanoliter doses into a soft tissue model [13].…”

Section: Introductionmentioning

confidence: 99%

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Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

et al. 2018

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Needle-free jet injection enables the delivery of drugs into skin or soft tissue by puncturing them with a high-velocity liquid jet. However, precise and efficient drug delivery requires generating such liquid jets with both a controlled velocity and a high throughput, which remains challenging with current spring-and gas-actuated jet injectors. Here, we propose a depth-controlled and high-throughput injection method by adapting laser-induced forward transfer (LIFT), a high-resolution two-dimensional printing technique, for direct three-dimensional liquid delivery into soft tissues.The velocity of thin liquid jets is laser actuated from 10 to 85 m/s so that doses as small as 10 pL, not achievable with other injectors, are injected at a 1 Hz repetition rate into a 300 μm thick soft gelatin substrate with a 25 μm depth precision and 12 μm lateral resolution. We further investigate the potential of this liquid delivery technique as a direct three-dimensional cell-delivery vehicle and show that depth-controlled particle delivery requires high-delivery efficiency. Our direct three-dimensional liquid delivery system opens up more possibilities for pinpoint drug delivery in soft tissues or tissue-engineered constructs.

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“…In the most classical approach, known as scaffold-based or top-down, scaffolds are fabricated through either conventional or additive manufacturing processes, and subsequently seeded with autologous or allogeneic cells. The cellular scaffold is then cultured in vitro to produce a tissue-engineered construct for subsequent implantation into the lesion site [66,116,146,177]. Despite the fact that such an approach allows for a good control over the scaffold characteristics (when additive manufacturing processes are used) and cellscaffold interactions, it fails in placing individual cells at specific locations throughout the scaffold, thereby not mimicking the intricate cellular organization of natural tissues at micro-and nanoscale.…”

Section: Introductionmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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Additive manufacturing of tissues and organs

Cited by 1,040 publications

References 171 publications

Powder bed generation in integrated modelling of additive layer manufacturing of orthopaedic implants

Powder bed generation in integrated modelling of additive layer manufacturing of orthopaedic implants

Depth-controlled laser-induced jet injection for direct three-dimensional liquid delivery

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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