Additive manufacturing of scaffolds with dexamethasone controlled release for enhanced bone regeneration

Costa, Pedro F.; Puga, Ana María Méndez; Díaz-Gómez, Luis; Concheiro, Angel; Busch, Dirk H.

doi:10.1016/j.ijpharm.2015.10.055

Cited by 65 publications

(48 citation statements)

References 41 publications

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“…2 However, it is a challenge to yield suitable poly(ε-caprolactone)-based biomaterials for tissue engineering purposes because poly(ε-caprolactone) has high hydrophobicity and low water adsorption. 3 Therefore, poly(ε-caprolactone) should be blended to polysaccharides (chitosan, 2 cellulose acetate, 4 and starch 5 ) or associated to materials with biological properties such as dexamethasone (notable for its anti-inflammatory properties) 6,7 to produce electrospun membranes for applications in tissue engineering field. These materials can enhance the cytocompatibility, promoting better anchorage of cells onto poly(ε-caprolactone)-based membrane surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…29,30 Scaffolds based on poly(ε-caprolactone)poloxamine loaded with dexamethasone also promoted release of dexamethasone and, hence fostered bone regeneration. 6 However, as suggested by many reports, dexamethasone must be avoided in real applications because of its disadvantages. 17,18 In this paper, we are envisioning to compare the biological properties of poly(ε-caprolactone), poly(ε-caprolactone)-chitosan and poly(ε-caprolactone)dexamethasone and show that the chitosan can replace dexamethasone in tissue engineering purposes.…”

Section: Introductionmentioning

confidence: 99%

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Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Machado

Roberto

Bonafé

et al. 2019

J. Braz. Chem. Soc.

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Poly(ε-caprolactone), an aliphatic polyester with biodegradability and cytocompatibility, has been used to create scaffolds for tissue engineering purposes. However, the hydrophobicity and low water absorptivity of poly(ε-caprolactone) reduce cell anchorage on their membranes. Here, poly(ε-caprolactone)-based scaffolds were prepared by electrospinning of poly(ε-caprolactone)chitosan blend, and poly(ε-caprolactone)-dexamethasone solution. Chitosan and dexamethasone play an essential role to increase the scaffolding performance of poly(ε-caprolactone)-based electrospun membranes. A poly(ε-caprolactone) membrane without chitosan and dexamethasone did not provide satisfactory results to promote cell culture of adipose mesenchymal stem cells (AMSCs). Compared to the poly(ε-caprolactone)-dexamethasone surface, poly(ε-caprolactone)chitosan membrane imparts better cytoskeletal reorganization, and cell spreading, increasing the strength of cell attachment. Also, poly(ε-caprolactone)-chitosan composite provides strong antimicrobial activity against Pseudomonas aeruginosa (ca. 90% inhibition). Therefore, the poly(ε-caprolactone)-chitosan composite is a better alternative to treat skin diseases and promote skin regeneration than conventional approaches based on dexamethasone.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Machado

Roberto

Bonafé

et al. 2019

J. Braz. Chem. Soc.

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“…According to the release kinetic results obtained (Figure 7), we can extrapolate that the concentrations of Dexa released in the culture media (using composite discs of 1.5 mg incubated in 200 µL cell culture medium) will range between 9.4×10 −7 M and 6.5×10 −9 M for PTMC/ PLA 2 and 6.4×10 −7 M and 6.7×10 −10 M for PTMC/PLA 1, which are in the bioactive concentration windows as previously mentioned. 24 In addition, after 5 weeks of incubation in PBS, the PTMC/PLA fiber composites were characterized using SEM (see Supplementary material Figure S1). These images clearly indicate that the composite structures were well preserved, with similar features to those initially observed on pristine composites ( Figure 5), for both surface and crosssection analyses.…”

Section: Ptmcmentioning

confidence: 99%

“…21 It also displays potent effects on the proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) in the presence of β-glycerol phosphate and ascorbic acid/ascorbate, 22,23 with optimal concentrations ranging from 10 to 100 nM. 24 Similarly, icaritin is a metabolite of the flavonoid glycoside extracted from Herba Epimedii, which was reported to enhance the differentiation and proliferation of osteoblasts. 25 Various systems have been developed as Dexa carriers, allowing a sustained release, such as implant dispensers, 26 nanoparticles/hydrogel complexes, 27 self-assembled nanofibrous gels, 28 electrospun fibers, 29 macroporous scaffolds, 30 and extrusion-based structures.…”

Section: Introductionmentioning

confidence: 99%

“…25 Various systems have been developed as Dexa carriers, allowing a sustained release, such as implant dispensers, 26 nanoparticles/hydrogel complexes, 27 self-assembled nanofibrous gels, 28 electrospun fibers, 29 macroporous scaffolds, 30 and extrusion-based structures. 24 Among these, Dexa-charged nanoparticles can easily be administered but have a low drugloading efficiency. It is also difficult to prevent their dispersion in the body following their administration for local treatment.…”

Section: Introductionmentioning

confidence: 99%

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A drug eluting poly(trimethylene carbonate)/poly(lactic acid)-reinforced nanocomposite for the functional delivery of osteogenic molecules

Zhang

Geven

Wang

et al. 2018

IJN

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BackgroundPoly(trimethylene carbonate) (PTMC) has wide biomedical applications in the field of tissue engineering, due to its biocompatibility and biodegradability features. Its common manufacturing involves photofabrication, such as stereolithography (SLA), which allows the fabrication of complex and controlled structures. Despite the great potential of SLA-fabricated scaffolds, very few examples of PTMC-based drug delivery systems fabricated using photo-fabrication can be found ascribed to light-triggered therapeutics instability, degradation, side reaction, binding to the macromers, etc. These concerns severely restrict the development of SLA-fabricated PTMC structures for drug delivery purposes.MethodsIn this context, we propose here, as a proof of concept, to load a drug model (dexamethasone) into electrospun fibers of poly(lactic acid), and then to integrate these bioactive fibers into the photo-crosslinkable resin of PTMC to produce hybrid films. The hybrid films’ properties and drug release profile were characterized; its biological activity was investigated via bone marrow mesenchymal stem cells culture and differentiation assays.ResultsThe polymer/polymer hybrids exhibit improved properties compared with PTMC-only films, in terms of mechanical performance and drug protection from UV denaturation. We further validated that the dexamethasone preserved its biological activity even after photoreaction within the PTMC/poly(lactic acid) hybrid structures by investigating bone marrow mesenchymal stem cells proliferation and osteogenic differentiation.ConclusionThis study demonstrates the potential of polymer–polymer scaffolds to simultaneously reinforce the mechanical properties of soft matrices and to load sensitive drugs in scaffolds that can be fabricated via additive manufacturing.

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Advanced biomaterials for periodontal tissue regeneration

Daghrery

Bottino

2022

Genesis

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Summary The periodontium is a suitable target for regenerative intervention, since it does not functionally restore itself after disease. Importantly, the limited regeneration capacity of the periodontium could be improved with the development of novel biomaterials and therapeutic strategies. Of note, the regenerative potential of the periodontium depends not only on its tissue‐specific architecture and function, but also on its ability to reconstruct distinct tissues and tissue interfaces, suggesting that the advancement of tissue engineering approaches can ultimately offer new perspectives to promote the organized reconstruction of soft and hard periodontal tissues. Here, we discuss material‐based, biologically active cues, and the application of innovative biofabrication technologies to regenerate the multiple tissues that comprise the periodontium.

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Additive manufacturing of scaffolds with dexamethasone controlled release for enhanced bone regeneration

Cited by 65 publications

References 41 publications

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

Chitosan Imparts Better Biological Properties for Poly(ε-caprolactone) Electrospun Membranes than Dexamethasone

A drug eluting poly(trimethylene carbonate)/poly(lactic acid)-reinforced nanocomposite for the functional delivery of osteogenic molecules

Advanced biomaterials for periodontal tissue regeneration

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