Additional microsatellites for <i>Sparus aurata</i> and cross‐species amplification within the Sparidae family

Brown, Cameron; Tsalavouta, Matina; Terzoglou, Vasso; Magoulas, A.; McAndrew, Brendan

doi:10.1111/j.1471-8286.2005.01007.x

Cited by 38 publications

(46 citation statements)

References 6 publications

(4 reference statements)

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“…As mentioned above, Sparidae are divided into two large clades (A and B in Orrell and Carpenter 2004). Crossspecies amplification of several microsatellite loci has been observed in this study as well as by Brown et al (2005) in sparid species belonging to both clades. The possibility of using the same markers across different species will speed up the construction of linkage maps in other sparids and allow easy comparisons between the different maps.…”

Section: Discussionmentioning

confidence: 91%

“…DQ851237-DQ851408). Six microsatellite loci published by Batargias et al (1999), one by Launey et al (2003), and six by Brown et al (2005) were also used. Twenty sea bream sequences containing a microsatellite repeat that were available in GenBank (AJ418632, AJ418650-AJ418652, AJ418655-AJ418656, AJ418660, AJ418665, AJ418667, AJ418670-AJ418673, AJ418675, AY138985, AF258447, and AF478568) were used to define primers, found polymorphic, and genotyped on the panel.…”

Section: Methodsmentioning

confidence: 99%

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A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

et al. 2006

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The gilthead sea bream (Sparus aurata L.) is a marine fish of great importance for fisheries and aquaculture. It has also a peculiar sex-determination system, being a protandrous hermaphrodite. Here we report the construction of a first-generation genetic linkage map for S. aurata, based on 204 microsatellite markers. Twenty-six linkage groups (LG) were found. The total map length was 1241.9 cM. The ratio between sex-specific map lengths was 1:1.2 (male:female). Comparison with a preliminary radiation hybrid (RH) map reveals a good concordance, as all markers located in a single LG are located in a single RH group, except for Ad-25 and CId-31. Comparison with the Tetraodon nigroviridis genome revealed a considerable number of evolutionary conserved regions (ECRs) between the two species. The mean size of ECRs was 182 bp (sequence identity 60-90%). Forty-one ECRs have a known chromosomal location in the pufferfish genome. Despite the limited number of anchoring points, significant syntenic relationships were found. The linkage map presented here provides a robust comparative framework for QTL analysis in S. aurata and is a step toward the identification of genetic loci involved both in the determination of economically important traits and in the individual timing of sex reversal. T HE gilthead sea bream (Sparus aurata L.) is a marine teleost fish that belongs to the family Sparidae. This family contains .100 species, which are divided into two large clades (groups A and B) according to the most recent molecular data (Orrell and Carpenter 2004). Sparids (porgies or sea breams) are demersal fish commonly found in temperate and tropical waters, with a maximum of diversity in the northeast Atlantic and the Mediterranean region (Bauchot and Hureau 1986), and they represent a key element of the coastal marine ecosystem. Sparids are also of great importance for fisheries and aquaculture, being excellent food fish, with high commercial value. S. aurata is the most prominent, with an average cultured production of 100 million metric tons per year. The great importance of the gilthead sea bream for marine aquaculture has fueled an increasing number of studies in many different areas such as immunology, endocrinology, bone morphology, and muscle physiology. Moreover, the genomic tool kit for S. aurata has been constantly improving, as a firstgeneration cDNA microarray was recently reported (Sarropoulou et al. 2005), a preliminary radiation hybrid (RH) map has been constructed (Senger et al. 2006), and a medium-scale expressed sequence tag (EST) sequencing project has been recently completed (A. Canario, personal communication). An improved RH map with .1000 markers (G. Kotoulas, personal communication) and an oligo array representing .10,000 unique transcripts (L. Bargelloni, personal communication) are expected by the end of 2006. Therefore, the genome of the gilthead sea bream is rapidly becoming one of the best characterized among teleost species, apart from classical models such as Brachydanio rerio (zebrafish...

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

et al. 2006

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“…DNA was preserved in 10 mM TE and stored in −20°C. A panel of 6 microsatellites was tested and optimized for the genetic analysis: SAI10, SAI12, SAI19 (Brown et al 2005) and Pma1, Pma2 and GA2A (Adcock et al 2000). Roques et al (2007) characterized an additional panel of species-specific microsatellites which ought to be under future consideration for screening.…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Population subdivision of saddled seabream Oblada melanura in the Aegean Sea revealed by genetic and morphometric analyses

Gkafas¹,

Tsigenopoulos²,

Magoulas³

et al. 2013

Aquat. Biol.

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The population structure of marine fishes is often cryptic, due to limited knowledge about life history and ecological habitats. Understanding environmental stability is a challenge to retrieve possible structuring patterns responsible for dispersal and demographic habits. In the present study, a combination of genetic and phenotypic assessments was applied in order to investigate population structure of the saddled seabream Oblada melanura in the Aegean Sea, eastern Mediterranean basin. Analyses of 6 microsatellite loci and 15 morphometric characters revealed that saddled seabream individuals differ among northern, southern and central Aegean populations. These observed boundaries may be related to the differentiated seascape of the Aegean Sea, suggesting that oceanographic factors are a significant stressor for population subdivision of the saddled seabream. Individual-based landscape genetic approaches and multivariate analysis of the morphometric characters suggest the presence of habitat-related limitations of saddled seabream dispersal potential. Molecular genetics and phenotypic analyses along with life-history traits provide useful informative data for the management and conservation schemes applied for this species in the Aegean Sea.KEY WORDS: Oblada melanura · Microsatellite · Morphometrics · Aegean Sea · Population structure Resale or republication not permitted without written consent of the publisher

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“…However, it is not clear how closely related the species must be. The term ''related species'' is used in a wide range of comparisons; some studies performed in fish have attempted cross-species amplifications across congeneric (within Symphodus, Arigoni and Largiader, 2000; within Lipophrys, Guillemaud et al 2000) or confamilial species (within Cyprinidae, Holmen et al 2005; within Sparidae, Brown et al 2005) with variable success. Genetic divergence is a clear and easy way to determine similarity between species and provides an accurate measure of their evolutionary distance.…”

Section: Introductionmentioning

confidence: 99%

Utility of pairwise mtDNA genetic distances for predicting cross-species microsatellite amplification and polymorphism success in fishes

2007

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In many studies involving microsatellites crossspecies amplification, primers designed for one (source) species are used to amplify homologous loci in related (target) species. However, it is not clear how closely related the species must be to attain significant success. Genetic divergence is a clear and easy way to assess similarity between species and provides an accurate measure of their evolutionary distance. Eight Mediterranean target species of the family Serranidae were analysed using twelve primers developed for Serranus cabrilla. Additionally, two mitochondrial genes (12S rRNA and 16S rRNA) were chosen on the basis of their extensive use in phylogenetic and evolutionary analyses to compute genetic divergence between the species. Significant negative correlations were found between genetic divergence and both cross-species amplification and maintained polymorphism of microsatellite markers, which could be generalized by gathering information from different fish studies. The success of obtaining amplifiable and polymorphic microsatellite loci can be a priori approximated knowing the mtDNA genetic divergence between a given source and target species using our inferred regression equations.

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Additional microsatellites for Sparus aurata and cross‐species amplification within the Sparidae family

Cited by 38 publications

References 6 publications

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

A Genetic Linkage Map of the Hermaphrodite Teleost Fish Sparus aurata L.

Population subdivision of saddled seabream Oblada melanura in the Aegean Sea revealed by genetic and morphometric analyses

Utility of pairwise mtDNA genetic distances for predicting cross-species microsatellite amplification and polymorphism success in fishes

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