Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality

Silva, D.M.; Zangerônimo, Márcio Gilberto; Murgas, Luís David Solis; Rocha, Luiz Célio Souza; Chaves, Bruna Resende; Pereira, Bárbara Azevedo; Cunha, Evandro César Pereira

doi:10.1016/j.ghir.2011.08.002

Cited by 16 publications

(16 citation statements)

References 29 publications

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“…This suggests a possible antioxidant effect of these substances in insemination doses stored for a prolonged period of time. The antioxidant effect of IGF-I has also been observed in buffaloes (Selvaraju et al, 2009), rodents (Gancarczyk et al, 2006), boars (Silva et al, 2011) and rabbits (Alvarez and Storey, 1987). These studies observed positive effects of IGF-I on glutathione peroxidase or superoxide dismutase activity.…”

Section: Discussionmentioning

confidence: 75%

“…This ensures that there are significant levels of this hormone in insemination doses before and after processing. Silva et al (2011), investigating the addition of IGF-I ranging from 50 to 150 ng/ml, recommended an optimal level of 150 ng/ml for insemination doses stored for 24 h and 78 ng/ml for doses stored for 72 h, but suggest that lower levels than those tested may also have physiological effects on boar semen. In the present work, 30 ng/ml was chosen because of previous observations that had showed an effect of this hormone on sperm motility, acrosomal damage and the MDA concentration in boar semen (unpublished observation).…”

Section: Discussionmentioning

confidence: 99%

“…All ejaculates fulfilled the minimum requirements for use in artificial insemination: .70% total motility, ,20% cytoplasmic droplets and total number of spermatozoa .200 3 10 6 /ml (Silva et al, 2011). After the initial evaluation, the semen was diluted 1 : 1 with a BTS (Beltsville Thawing Solution R , Porto Alegre, Brazil) extender at 378C.…”

Section: Methodsmentioning

confidence: 99%

“…Several chemical substances have been linked to metabolic control of the sperm cell, including the growth factor insulin-like type I (IGF-I) in bovine (Henricks et al, 1998) and swine (Silva et al, 2011). According to previous studies in buffalo, this substance acts as a metabolic activator of sperm that is involved in cell movement (motility and intensity of movement) by increasing carbohydrate metabolism, and consequently, the fertilising capacity of the sperm (Selvaraju et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…It has also been shown that, immediately after the addition of this hormone in semen, sperm quality is improved in equine (Macpherson et al, 2002), buffalo (Selvaraju et al, 2009) and boar (Silva et al, 2011). Thus, a method to improve the quality of cooled boar semen could be the addition of a metabolic activator such as IGF-I, along with an antioxidant such as vitamin E.…”

Section: Introductionmentioning

confidence: 99%

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Effect of the addition of IGF-I and vitamin E to stored boar semen

Mendez

Zangerônimo

Rocha

et al. 2013

Animal

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The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 158C, in conjunction with the addition of different amounts of vitamin E (a-tocopherol). Semen samples (n 5 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 mg/ml. Immediately after processing and after the doses had been stored at 158C for 24 or 72 h, samples were warmed at 378C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 mg/ml of vitamin E to diluted semen reduced ( P , 0.01) the malondialdehyde (MDA) production in boar semen stored at 158C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 378C. In these conditions, IGF-I also reduced ( P , 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced ( P 5 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 mg/ml of vitamin E reduces the MDA production in boar semen stored at 158C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 378C.

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Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of the addition of IGF-I and vitamin E to stored boar semen

Mendez

Zangerônimo

Rocha

et al. 2013

Animal

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FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

Wang

Baloch

Zhang

et al. 2019

Journal Cellular Physiology

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The fusion of sperm and oocytes determines the fertilization competence and subsequent development of embryos, which, in turn, can be affected by various proteins and DNA methylation. However, several factors in this whole regulation process remain unknown, especially in yaks. Here, we report that fibroblast growth factor 10 (FGF10) is an important growth factor that can enhance the maturation rate of yak oocytes and the motility of frozen spermatozoa. Subsequent blastocyst quality was also improved by increasing the total cell number and level of pregnancy‐associated protein in blastocysts. These effects were significantly high in the group that received the 5 ng/ml FGF10 treatment, during both in vitro maturation (IVM) and capacitation. Our data show that the effects of FGF10 were dose‐dependent at vital steps of embryogenesis in vitro. Furthermore, quantitative polymerase chain reaction, western blot analysis, and immunofluorescence demonstrated that the levels of CD9, CD81, DNMT1, and DNMT3B in both mature cumulus‐oocyte complexes and capacitated sperms were regulated by FGF10, which was also highly expressed in the group treated with 5 ng/ml FGF10 during both IVM and capacitation. From our present study, we concluded that FGF10 promotes yak oocyte fertilization competence and subsequent blastocyst quality, and could also regulate CD9, CD81, DNMT1, and DNMT3B to optimize sperm–oocyte interactions and DNA methylation during fertilization.

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Quality Improvement of Boar Seminal Doses

Bussalleu

Torner

2013

Boar Reproduction

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Addition of IGF-I to storage-cooled boar semen and its effect on sperm quality

Cited by 16 publications

References 29 publications

Effect of the addition of IGF-I and vitamin E to stored boar semen

Effect of the addition of IGF-I and vitamin E to stored boar semen

FGF10 enhances yak oocyte fertilization competence and subsequent blastocyst quality and regulates the levels of CD9, CD81, DNMT1, and DNMT3B

Quality Improvement of Boar Seminal Doses

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