Addition of glycerol enhances the flexibility of gelatin hydrogel sheets; application for in utero tissue engineering

Watanabe, Miho; Li, Haiying; Yamamoto, Masaya; Horinaka, Jun ichi; Tabata, Yasuhiko; Flake, Alan W.

doi:10.1002/jbm.b.34756

Cited by 12 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fetal Trocar Passing through Test: SMP strings of 2.0 mm thickness along with a 10-Fr fetoscopic cannula sleeve (COOK Medical Corp., USA) were used in the tests of the ability of the SMP strings to pass through the endoscopic trocar, method referred to previous report. [54] The SMP strings were tied with a square knot as in the previous contraction tests and inserted through a 10-Fr endoscopic cannula sleeve with the assistance of a plunger. SMP strings were deployed to confirm the intact result.…”

Section: Methodsmentioning

confidence: 99%

Smart Shape‐Memory Polymeric String for the Contraction of Blood Vessels in Fetal Surgery of Sacrococcygeal Teratoma

Fulati

Uto

Iwanaga

et al. 2022

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Shape-memory polymers (SMPs) are promising materials in numerous emerging biomedical applications owing to their unique shape-memory characteristics. However, simultaneous realization of high strength, toughness, stretchability while maintaining high shape fixity (R f ) and shape recovery ratio (R r ) remains a challenge that hinders their practical applications. Herein, a novel shape-memory polymeric string (SMP string) that is ultra-stretchable (up to 1570%), strong (up to 345 MPa), tough (up to 237.9 MJ m −3 ), and highly recoverable (R f averagely above 99.5%, R r averagely above 99.1%) through a facile approach fabricated solely by tetra-branched poly(𝝐-caprolactone) (PCL) is reported. Notably, the shape-memory contraction force (up to 7.97 N) of this SMP string is customizable with the manipulation of their energy storage capacity by adjusting the string thickness and stretchability. In addition, this SMP string displays a controllable shape-memory response time and demonstrates excellent shape-memory-induced contraction effect against both rigid silicone tubes and porcine carotids. This novel SMP string is envisioned to be applied in the contraction of blood vessels and resolves the difficulties in the restriction of blood flow in minimally invasive surgeries such as fetoscopic surgery of sacrococcygeal teratoma (SCT).

show abstract

Section: Methodsmentioning

confidence: 99%

Smart Shape‐Memory Polymeric String for the Contraction of Blood Vessels in Fetal Surgery of Sacrococcygeal Teratoma

Fulati

Uto

Iwanaga

et al. 2022

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“… 33 Gelatin is a biopolymer naturally derived from collagen with relatively low biotoxicity and acceptable degradation rate. 34 , 35 According to recent reports, gels fabricated based on the combination of two or more different polymers via cross-linkers can tackle disadvantages and integrate advantages. 36–39 Therefore, we tried to add gels to alginate to fabricate composite hydrogel scaffolds.…”

Section: Introductionmentioning

confidence: 99%

3D Printed Gelatin/Sodium Alginate Hydrogel Scaffolds Doped with Nano-Attapulgite for Bone Tissue Repair

Liu¹,

Qin²,

Wang³

et al. 2021

IJN

View full text Add to dashboard Cite

Introduction Bone tissue engineering (BTE) is a new strategy for bone defect repair, but the difficulties in the fabrication of scaffolds with personalized structures still limited their clinical applications. The rapid development in three-dimensional (3D) printing endows it capable of controlling the porous structures of scaffolds with high structural complexity and provides flexibility to meet specific needs of bone repair. Methods In this study, sodium alginate (SA)/gelatin (Gel) hydrogel scaffolds doped with different contents of nano-attapulgite were fabricated via 3D printing. The surface microstructure, hydrophilicity and mechanical properties were fully evaluated. Furthermore, mouse bone marrow-derived mesenchymal stem cells (BMSCs) were cultured with the composite hydrogels in vitro, and proliferation and osteoblastic differentiation were assessed. A rabbit tibia plateau defect model was used to evaluate the osteogenic potential of the composite hydrogel in vivo. Results When increasing nano-ATP content, the Gel/SA/nano-ATP composite hydrogels showed better mechanical property and printability. Moreover, Gel/SA/nano-ATP composite hydrogels showed excellent bioactivity, and a significant mineralization effect was observed on the surface after being incubated in simulated body fluid (SBF) for 14 days. The Gel/SA/nano-ATP composite hydrogel also showed good biocompatibility and promoted the osteogenesis of BMSCs. Finally, histological analysis demonstrates that the Gel/SA/nano-ATP composite hydrogels could effectively enhance bone regeneration in vivo. Conclusion These properties render the Gel/SA/nano-ATP composite hydrogel scaffolds an ideal bone tissue engineering material for the repair of bone defects.

show abstract

“…While gelatin films, either uncrosslinked ( Gel ) or crosslinked with mTG ( MxGel ), are typically brittle when dry, which can lead to cracking, the addition of glycerol improves flexibility and bending. Low-MW plasticizers such as glycerol increase flexibility due to increased porosity and mobility of the polymeric chain . Glycerol has been shown to more than double mTG half-life under heating, improving activity at the temperatures needed to keep gelatin a liquid to allow for mixing of solutions and subsequent crosslinking .…”

Section: Resultsmentioning

confidence: 99%

Stretchable and Electroactive Crosslinked Gelatin for Biodevice and Cell Culture Applications

Brooks

Wulff

Broitman

et al. 2022

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Biomimetic substrates that incorporate functionality such as electroactivity and mechanical flexibility, find utility in a variety of biomedical applications. Toward these uses, naturederived materials such as gelatin offer inherent biocompatibility and sustainable sourcing. However, issues such as high swelling, poor mechanical properties, and lack of stability at biological temperatures limit their use. The enzymatic crosslinking of gelatin via microbial transglutaminase (mTG) yields flexible and robust large area substrates that are stable under physiological conditions. Here, we demonstrate the fabrication and characterization of strong, stretchable, conductive mTG crosslinked gelatin thin films. Incorporation of the conductive polymer poly(3,4-ethylenedioxythiophene) polystyrene sulfonate in the gel matrix with a bioinspired polydopamine surface coating is used to enable conductivity with enhanced mechanical properties such as extensibility and flexibility, in comparison to plain gelatin or crosslinked gelatin films. The electroconductive substrates are conducive to cell growth, supporting myoblast cell adhesion, viability, and proliferation and could find use in creating active cell culture systems incorporating electrical stimulation. The substrates are responsive to motion such as stretching and bending while being extremely handleable and elastic, making them useful for applications such as electronic skin and flexible bioelectronics. Overall, this work presents facile, yet effective development of bioinspired conductive composites as substrates for bio-integrated devices and functional tissue engineering.

show abstract

Addition of glycerol enhances the flexibility of gelatin hydrogel sheets; application for in utero tissue engineering

Cited by 12 publications

References 30 publications

Smart Shape‐Memory Polymeric String for the Contraction of Blood Vessels in Fetal Surgery of Sacrococcygeal Teratoma

Smart Shape‐Memory Polymeric String for the Contraction of Blood Vessels in Fetal Surgery of Sacrococcygeal Teratoma

3D Printed Gelatin/Sodium Alginate Hydrogel Scaffolds Doped with Nano-Attapulgite for Bone Tissue Repair

Stretchable and Electroactive Crosslinked Gelatin for Biodevice and Cell Culture Applications

Contact Info

Product

Resources

About