Adding hormones sequentially could be an effective approach for IVM of dog oocytes

Evecen, Mithat; Cirit, Ümüt; Demır, Kadir; Hamzaoğlu, Asiye İzem; Bakirer, Gül; Pabucçuoğlu, Serhat; Birler, Sema

doi:10.1016/j.theriogenology.2011.01.004

Cited by 13 publications

(11 citation statements)

References 16 publications

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“…The maturation rates (MII) obtained in the bi-phasic systems were consistent with those reported in the literature. Although they were lower than expected, they were still higher than those reported by Evecen et al (2011) who also used a sequential medium with gonadotrophins and steroids (20% vs 5.5%). These differences could be attributed to the distinct base medium used in each study (synthetic oviduct fluid þ BSA vs medium 199 þ FBS) and also to the differences between laboratory conditions.…”

Section: Discussioncontrasting

confidence: 66%

“…These results prompted to the investigation of a bi-phasic system using a combination of different hormones; the results of a pilot study indicated that oocytes matured in sequential media with hormones presented higher MII compared with those exposed to hormones in a continuous way (Apparicio et al 2009). Recently, this evidence was confirmed by a study of Evecen et al (2011), in which metaphase I (MI)þMII rates were higher among oocytes cultured in sequential media than those matured in the traditional way. However, in the aforementioned studies only nuclear maturation was evaluated and no information was available on cytoplasmic changes during culture.…”

Section: Introductionmentioning

confidence: 85%

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Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

Apparício

Mostachio

Motheo

et al. 2015

Reprod. Fertil. Dev.

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The aim of this study was to evaluate the influence of different bi-phasic systems with gonadotrophins and steroids on in vitro maturation rates of oocytes obtained from bitches at different reproductive stages (follicular, luteal, anoestrous). In System A (control) oocytes were matured for 72h in base medium (BM) with 10IUmL(-1) human chorionic gonadotrophin (hCG), 1μgmL(-1) progesterone (P4) and 1μgmL(-1) oestradiol (E2); in bi-phasic System B oocytes were matured for 48h in BM with hCG and for 24h in BM with P4; in bi-phasic System C oocytes were matured for 48h in BM with hCG, P4 and E2, and for 24h in BM with P4; in System D, oocytes were cultured in BM without hormonal supplementation. Data were analysed by ANOVA. There was a positive effect of the bi-phasic systems on germinal vesicle breakdown, metaphase I and metaphase II rates, irrespective of reproductive status (P<0.05). Bi-phasic systems were also beneficial for cortical granule distribution (an indication of cytoplasmic maturation) and its relationship to nuclear status: 74.5% of the oocytes cultured in System B and 85.4% of those cultured in System C presented both nuclear and cytoplasmic maturation (P<0.001). The stage of the oestrous cycle did not influence maturation rates.

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Section: Discussioncontrasting

confidence: 66%

Section: Introductionmentioning

confidence: 85%

Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

Apparício

Mostachio

Motheo

et al. 2015

Reprod. Fertil. Dev.

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“…Furthermore, the success of in vitro technologies is still very limited in dogs (4,8). Previously, our attempts to mimic the in vivo hormone secretion mechanism of dogs showed an in vitro maturation rate lower than 50% (9). The low IVM rates in dogs are the main cause of IVF and subsequent embryonic development failures (4).…”

Section: Introductionmentioning

confidence: 99%

“…Only seven (14) and six (15) healthy puppies were delivered in the two most successful trials until date. Reliable systems for in vitro embryo production have not yet been developed, and new approaches are needed for dogs (9,11). The cost of pituitary gonadotropins (FSH and LH) is considered an obstacle for in vitro studies in dogs, with low success rates as well.…”

Section: Introductionmentioning

confidence: 99%

In vitro effect of recombinant human gonadotropins on meiotic competence of dog oocytes

Demır¹,

Evecen²,

Arıcı³

et al. 2019

Turk J Vet Anim Sci

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The reproductive biology of the domestic dog is unique among mammalian species; because of this, in vitro maturation (IVM) rate is still very low compared with other domestic animals in spite of attempts at improvement. The aim of this study was to consider the use of recombinant human gonadotropins as a replacement for pituitary gonadotropins for IVM of dog oocytes. A total of 845 cumulusoocyte complexes were used in this study. To determine the effects of human recombinant gonadotropins, maturation medium was supplemented with two different concentrations (0.05 or 0.1 IU/mL) of pituitary (pFSH, pLH) and human recombinant (rhFSH and rhLH) gonadotropins. After the IVM period, the maturation rate of the oocytes was investigated under an epifluorescence microscope. Our findings showed no significant difference in maturation rate using either pituitary or human recombinant gonadotropin groups (P > 0.05). Applying 1.0 IU human recombinant gonadotropin caused the lowest maturation rate (34.57%; P < 0.05). In conclusion, recombinant human gonadotropins could be applied for IVM of dog oocytes. Moreover, 0.05 IU/mL rhFSH and rhLH can be successfully used in place of biologically derived hormones.

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“…It is common for 60–80% of healthy‐appearing, germinal vesicle (GV) stage domestic cat (Godard et al, 2009), mouse (Marín Bivens et al, 2004), goat (Khatun et al, 2011), sheep (Shirazi et al, 2010), pig (Koike et al, 2010), and cow (Sirard et al, 1988) oocytes to transition from the GV stage to MII in vitro. Under these same laboratory conditions and with studies conducted by multiple, independent laboratories, however, only 20% of dog oocytes generally reach the MII stage (Mahi and Yanagimachi, 1976; Shimazu et al, 1992; Nickson et al, 1993; Yamada et al, 1993; Bolamba et al, 1998; Otoi et al, 2000; Luvoni et al, 2001; Saint‐Dizier et al, 2001; Kim et al, 2004; Songsasen and Wildt, 2007; Alhaider and Watson, 2009; Rodrigues et al, 2009; Silva et al, 2010; Evecen et al, 2011). One factor identified as a major influence on IVM success of the dog oocyte is the size of the donor follicle, where larger follicles provide oocytes with higher meiotic competency (Songsasen and Wildt, 2005).…”

Section: Introductionmentioning

confidence: 99%

The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation

Songsasen

Wesselowski

Carpenter

et al. 2011

Molecular Reproduction Devel

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We tested the hypothesis that meiotic competence of dog oocytes was tightly linked with donor follicle size and energy metabolism. Oocytes were recovered from small (< 1 mm diameter, n = 327), medium (1 – < 2 mm, n = 292) or large (≥ 2 mm, n = 102) follicles, cultured for 0, 24 or 48 h, assessed for glycolysis, glucose oxidation, pyruvate uptake, glutamine oxidation and then nuclear status. More oocytes (P < 0.05) from large follicles (37%) reached the metaphase II (MII) stage than from the small group (11%), with the medium size class being intermediate (18%; P > 0.05). Glycolytic rate increased (P < 0.05) as oocytes progressed from the germinal vesicle (GV) to MII stage. At 48 h of culture, oocytes completing nuclear maturation had higher (P < 0.05) glycolytic rate than those arrested at earlier stages. GV oocytes recovered from large follicle oocytes had higher (P < 0.05) metabolism than those from smaller counterparts at culture onset. MII oocytes from large follicles oxidized more (P < 0.05) glutamine than the same stage gametes recovered from smaller counterparts. In summary, larger size dog follicles contain a more metabolically-active oocyte with a greater chance of achieving nuclear maturation in vitro. These findings demonstrate a significant role of energy metabolism in promoting dog oocyte maturation, information that will be useful for improving culture systems for rescuing intraovarian genetic material.

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Adding hormones sequentially could be an effective approach for IVM of dog oocytes

Cited by 13 publications

References 16 publications

Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

Distribution of cortical granules and meiotic maturation of canine oocytes in bi-phasic systems

In vitro effect of recombinant human gonadotropins on meiotic competence of dog oocytes

The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation

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