Add Some Fat to Vascular Progenitor Cell Therapy

Saif, Jaimy; Heeschen, Christopher; Aicher, Alexandra

doi:10.1161/circresaha.109.200469

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…Mural cells stabilize the process of blood vessel maturation. Bone marrow, peripheral blood and vessel wall—derived cells which are autologous and cord blood from allogeneic sources as vascular progenitor cells have been identified and tested in preclinical models for this purpose . It has previously been shown that the co‐culture of adipose stromal cells and endothelial cells demonstrated the formation of denser and more stable vascular network and this is consistent with the observations in our study using hCDSC and HUVECS.…”

Section: Discussionsupporting

confidence: 91%

Pro‐angiogenic potential of human chorion‐derived stem cells: in vitro and in vivo evaluation

Fariha

Chua

Tan

et al. 2013

J Cellular Molecular Medi

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Human chorion-derived stem cells (hCDSC) were previously shown to demonstrate multipotent properties with promising angiogenic characteristics in monolayer-cell culture system. In our study, we investigated the angiogenic capability of hCDSC in 3-dimensional (3D) in vitro and in vivo angiogenic models for the purpose of future application in the treatment of ischaemic diseases. Human CDSC were evaluated for angiogenic and endogenic genes expressions by quantitative PCR. Growth factors secretions were quantified using ELISA. In vitro and in vivo vascular formations were evaluated by histological analysis and confocal microscopic imaging. PECAM-1+ and vWF+ vascular-like structures were observed in both in vitro and in vivo angiogenesis models. High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC. The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues. Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

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Section: Discussionsupporting

confidence: 91%

Pro‐angiogenic potential of human chorion‐derived stem cells: in vitro and in vivo evaluation

Fariha

Chua

Tan

et al. 2013

J Cellular Molecular Medi

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Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent

Dore‐Duffy¹,

Mehedi²,

Wang³

et al. 2011

Microvascular Research

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Despite their identification more than 100 years ago by the French scientist Charles-Marie Benjamin Rouget, microvascular pericytes have proven difficult to functionally characterize, due in part to their relatively low numbers and the lack of specific cell markers. However, recent progress is beginning to shed light on the diverse biological functions of these cells. Pericytes are thought to be involved in regulating vascular homeostasis and hemostasis as well as serving as a local source of adult stem cells. To further define the properties of these intriguing cells, we have isolated pericytes from transgenic mice (Immortomouse®) harboring a temperature-sensitive mutant of the SV40 virus target T-gene. This Immortopericyte (IMP) conditional cell line is stable for long periods of time and, at 33°C in the presence of interferon gamma, does not differentiate. Under these conditions IMPs are alpha muscle actin-negative and exhibit a pluripotent phenotype, but can be induced to differentiate along both mesenchymal and neuronal lineages at 37°C. Alternatively, differentiation of wild type pericytes and IMPs can be induced directly from capillaries in culture. Finally, the addition of endothelial cells to purified IMP cultures augments their rate of self-renewal and differentiation, possibly in a cell-to-cell contact dependent manner.

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Pericytes at the intersection between tissue regeneration and pathology: Figure 1

et al. 2015

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Perivascular multipotent cells, pericytes, contribute to the generation and repair of various tissues in response to injury. They are heterogeneous in their morphology, distribution, origin and markers, and elucidating their molecular and cellular differences may inform novel treatments for disorders in which tissue regeneration is either impaired or excessive. Moreover, these discoveries offer novel cellular targets for therapeutic approaches to many diseases. This review discusses recent studies that support the concept that pericyte subtypes play a distinctive role in myogenesis, neurogenesis, adipogenesis, fibrogenesis and angiogenesis.

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Add Some Fat to Vascular Progenitor Cell Therapy

Cited by 6 publications

References 21 publications

Pro‐angiogenic potential of human chorion‐derived stem cells: in vitro and in vivo evaluation

Pro‐angiogenic potential of human chorion‐derived stem cells: in vitro and in vivo evaluation

Immortalized CNS pericytes are quiescent smooth muscle actin-negative and pluripotent

Pericytes at the intersection between tissue regeneration and pathology: Figure 1

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