Adaptive response to ionizing radiation in human lymphocytes: the problem of scoring aberrations in cells irradiated during asynchronous growth

Wójcik, Andrzej; Aghamohammadi, S.Z.; Aillaud, Marie-Françoise; Bosi, A.; Dai, Guoqiang; Olivieri, G.; Salone, B.; Savage, John R. K.; Shadley, Jeff D.; Streffer, C.

doi:10.1016/s0165-1110(96)90034-2

Cited by 16 publications

(2 citation statements)

References 17 publications

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“…Th e method of sequential harvesting, also known as the multiple fi xation regimen, was recommended to obtain a meaningful frequency of chromosomal aberrations in cells irradiated during asynchronous growth (Kaufman et al 1974, Savage andPapworth 1991). Th e underlying principle of this method is that it allows identifying increased or reduced levels of cytogenetic damage as being caused by transient shifts of the cell cycle if they are not observed in all samples harvested sequentially (Wojcik et al 1996). Indeed, the results of our sequential harvesting experiments clearly show that the TE observed in TK6 cells after a harvesting time of 27 h was not seen at later harvesting times (Dang et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells

Lisowska¹,

Brehwens²,

Zölzer³

et al. 2014

International Journal of Radiation Biology

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Section: Resultsmentioning

confidence: 99%

Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells

Lisowska¹,

Brehwens²,

Zölzer³

et al. 2014

International Journal of Radiation Biology

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“…The major problem is that cells progress through the cell cycle with variable speed, hence a simple relationship between the time of treatment plus the adding of colcemid and the phase of the cell cycle during which the treatment occurred is not possible. Nevertheless, when aberrations are analyzed on a series of slides prepared after increasing times post treatment, the mean yield of chromatid aberration declines with post-treatment time [ 21 – 23 ]. This result is interpreted as a reflection of the increasing radiosensitivity of cells as they progress from S-phase towards mitosis [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Application of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay

et al. 2016

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Among the numerous methods available to assess genotoxicity, the cytokinesis-block micronucleus (CBMN) assay is very popular due its relative simplicity and power to detect both clastogenic and aneugenic compounds. A problem with the CBMN assay is that all DNA damaging agents also inhibit the ability of cells to progress through mitosis, leading to a low number of binucleated cells (BNCs). One method to resolve this issue is to ensure a sufficient proportion of BNCs in the samples. In the current study, the applicability of a cell sorting system capable of isolating cell fractions containing abundant BNCs was investigated. Furthermore, to investigate the relationship between the cell division delay due to radiation exposure and the generation of BNCs and micronuclei (MN), we assessed a series of lag times between radiation exposure and addition of cytochalasin-B (Cyt-B). Cells from the human chronic myelogenous leukemia cell line K562 were exposed to X-rays (2 Gy and 4 Gy), and Cyt-B was subsequently added at 0, 6 and 12 h following irradiation. After treatment with Cyt-B for 24 h, the percentage of BNCs, the MN frequency and the cell cycle distribution were analyzed. In addition, cells displaying the DNA contents corresponding to BNCs were isolated and analyzed. The results indicate that applying the cell sorter to the CBMN assay increased the percentage of BNCs compared with the standard method. Thus, this technique is a promising way of enhancing the capacity of the CBMN assay.

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Radioadaptive response revisited

Tapio

Jacob

2006

Radiat Environ Biophys

155

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Radiation-induced adaptive response belongs to the group of non-targeted effects that do not require direct exposure of the cell nucleus by radiation. It is described as the reduced damaging effect of a challenging radiation dose when induced by a previous low priming dose. Adaptive responses have been observed in vitro and in vivo using various indicators of cellular damage, such as cell lethality, chromosomal aberrations, mutation induction, radiosensitivity, and DNA repair. Adaptive response can be divided into three successive biological phenomena, the intracellular response, the extracellular signal, and the maintenance. The intracellular response leading to adaptation of a single cell is a complex biological process including induction or suppression of gene groups. An extracellular signal, the nature of which is unknown, may be sent by the affected cell to neighbouring cells causing them to adapt as well. This occurs either by a release of diffusible signalling molecules or by gap-junction intercellular communication. Adaptive response can be maintained for periods ranging from of a few hours to several months. Constantly increased levels of reactive oxygen species (ROS) or nitric oxide (NO) have been observed in adapted cells and both factors may play a role in the maintenance process. Although adaptive response seems to function by an on/off principle, it is a phenomenon showing a high degree of inter- and intraindividual variability. It remains to be seen to what extent adaptive response is functional in humans at relevant dose and dose-rate exposures. A better understanding of adaptive response and other non-targeted effects is needed before they can be confirmed as risk estimate factors for the human population at low levels of ionising radiation.

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Adaptive response to ionizing radiation in human lymphocytes: the problem of scoring aberrations in cells irradiated during asynchronous growth

Cited by 16 publications

References 17 publications

Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells

Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells

Application of cell sorting for enhancing the performance of the cytokinesis-block micronucleus assay

Radioadaptive response revisited

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