Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells

Lisowska, Halina; Brehwens, Karl; Zölzer, Friedo; Węgierek-Ciuk, Aneta; Czub, Joanna; Lankoff, Anna; Haghdoost, Siamak; Wójcik, Andrzej

doi:10.3109/09553002.2014.887233

Cited by 15 publications

(7 citation statements)

References 26 publications

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“…Irradiation of tardigrades at different temperatures did not influence survival rates but low temperature resulted in a delay by 12 to 24 hours in the effect of gamma radiation. Irradiation on ice is a common procedure in many experiments of mammalian cells in order to reduce activation of DNA repair, along with other cellular processes, during the radiation exposure [ 34 , 35 ]. Recent studies have also shown that dehydration and low temperatures (below freezing) can provide protection to radiation damage by reducing the diffusion of free radicals [ 36 – 38 ].…”

Section: Discussionmentioning

confidence: 99%

Tolerance to Gamma Radiation in the Tardigrade Hypsibius dujardini from Embryo to Adult Correlate Inversely with Cellular Proliferation

Beltrãn-Pardo¹,

Jonsson²,

Harms‐Ringdahl³

et al. 2015

PLoS ONE

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Tardigrades are highly tolerant to desiccation and ionizing radiation but the mechanisms of this tolerance are not well understood. In this paper, we report studies on dose responses of adults and eggs of the tardigrade Hypsibius dujardini exposed to gamma radiation. In adults the LD50/48h for survival was estimated at ~ 4200 Gy, and doses higher than 100 Gy reduced both fertility and hatchability of laid eggs drastically. We also evaluated the effect of radiation (doses 50 Gy, 200 Gy, 500 Gy) on eggs in the early and late embryonic stage of development, and observed a reduced hatchability in the early stage, while no effect was found in the late stage of development. Survival of juveniles from irradiated eggs was highly affected by a 500 Gy dose, both in the early and the late stage. Juveniles hatched from eggs irradiated at 50 Gy and 200 Gy developed into adults and produced offspring, but their fertility was reduced compared to the controls. Finally we measured the effect of low temperature during irradiation at 4000 Gy and 4500 Gy on survival in adult tardigrades, and observed a slight delay in the expressed mortality when tardigrades were irradiated on ice. Since H. dujardini is a freshwater tardigrade with lower tolerance to desiccation compared to limno-terrestrial tardigrades, the high radiation tolerance in adults, similar to limno-terrestrial tardigrades, is unexpected and seems to challenge the idea that desiccation and radiation tolerance rely on the same molecular mechanisms. We suggest that the higher radiation tolerance in adults and late stage embryos of H. dujardini (and in other studied tardigrades) compared to early stage embryos may partly be due to limited mitotic activity, since tardigrades have a low degree of somatic cell division (eutely), and dividing cells are known to be more sensitive to radiation.

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Section: Discussionmentioning

confidence: 99%

Tolerance to Gamma Radiation in the Tardigrade Hypsibius dujardini from Embryo to Adult Correlate Inversely with Cellular Proliferation

Beltrãn-Pardo¹,

Jonsson²,

Harms‐Ringdahl³

et al. 2015

PLoS ONE

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“…In recent years, Baird and colleagues showed that hypothermia (13 °C) postpones DNA damage repair in irradiated BJ-hTERT cells and protects against cell death [ 75 ]. By contrast, a series of studies investigating hypothermia-induced DNA damage in human peripheral blood lymphocytes [ 76 , 77 , 78 , 79 , 80 ] has suggested the possibility of DNA repair promotion that leads to a reduced transformation of DNA damage to chromosomal aberrations. Therefore, to our knowledge, hypothermia-induced modulations of DNA damage repair in vitro remain controversial.…”

Section: Impairment Of Radiation Damage Repair Under Hypothermic Cmentioning

confidence: 99%

The Effect of Low Temperatures on Environmental Radiation Damage in Living Systems: Does Hypothermia Show Promise for Space Travel?

Fukunaga

2020

IJMS

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Low-temperature treatments (i.e., hypothermia) may be one way of regulating environmental radiation damage in living systems. With this in mind, hibernation under hypothermic conditions has been proposed as a useful approach for long-term human space flight. However, the underlying mechanisms of hypothermia-induced radioresistance are as yet undetermined, and the conventional risk assessment of radiation exposure during hibernation remains insufficient for estimating the effects of chronic exposure to galactic cosmic rays (GCRs). To promote scientific discussions on the application of hibernation in space travel, this literature review provides an overview of the progress to date in the interdisciplinary research field of radiation biology and hypothermia and addresses possible issues related to hypothermic treatments as countermeasures against GCRs. At present, there are concerns about the potential effects of chronic radiation exposure on neurological disorders, carcinogenesis, ischemia heat failures, and infertility in astronauts; these require further study. These concerns may be resolved by comparing and integrating data gleaned from experimental and epidemiological studies.

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“…During hibernation, a remarkable resistance against radiation damage has been observed which could be helpful to counteract the radiation load during spaceflights [ 14 , 116 ]. Hypothermic conditions in which core body temperature decreases well below 37 °C provide some protection against radiation-induced damage [ 14 , 62 ] independent of whether hypothermia is applied before, during, or shortly after irradiation of cultured cells [ 62 ]. It was hypothesized that radioprotection during torpor may be the result of tissue hypoxia due to vasoconstriction [ 116 ] and decreased free radical production [ 21 ] as well as synergistically by reduced MR and a shift from glycolysis to lipolysis mimicking conditions during fasting [ 15 ].…”

Section: Protective Effects Of Hypometabolismmentioning

confidence: 99%

Hibernating astronauts—science or fiction?

Choukèr

Bereiter‐Hahn

Singer

et al. 2018

Pflugers Arch - Eur J Physiol

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For long-duration manned space missions to Mars and beyond, reduction of astronaut metabolism by torpor, the metabolic state during hibernation of animals, would be a game changer: Water and food intake could be reduced by up to 75% and thus reducing payload of the spacecraft. Metabolic rate reduction in natural torpor is linked to profound changes in biochemical processes, i.e., shift from glycolysis to lipolysis and ketone utilization, intensive but reversible alterations in organs like the brain and kidney, and in heart rate control via Ca 2+. This state would prevent degenerative processes due to organ disuse and increase resistance against radiation defects. Neuro-endocrine factors have been identified as main targets to induce torpor although the exact mechanisms are not known yet. The widespread occurrence of torpor in mammals and examples of human hypometabolic states support the idea of human torpor and its beneficial applications in medicine and space exploration.

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Effect of hypothermia on radiation-induced micronuclei and delay of cell cycle progression in TK6 cells

Abstract: The protective effect of hypothermia observed at the level of cytogenetic damage was not due to a modulation of cell cycle progression. A possible alternative mechanism and experiments to test it are discussed.

Cited by 15 publications

References 26 publications

Tolerance to Gamma Radiation in the Tardigrade Hypsibius dujardini from Embryo to Adult Correlate Inversely with Cellular Proliferation

Tolerance to Gamma Radiation in the Tardigrade Hypsibius dujardini from Embryo to Adult Correlate Inversely with Cellular Proliferation

The Effect of Low Temperatures on Environmental Radiation Damage in Living Systems: Does Hypothermia Show Promise for Space Travel?

Hibernating astronauts—science or fiction?

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