Adaptive optics in microscopy

Booth, Martin J.

doi:10.1098/rsta.2007.0013

Cited by 428 publications

(278 citation statements)

References 92 publications

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“…Done correctly, this should reduce the pixel-to-pixel illumination difference dramatically, removing the artifacts across IR images. We will finally be able to exploit the order of magnitude spectral advantage in signalto-noise expected per single FPA pixel at the highest magnification and image oversampling [8,9]. Currently under commissioning, the aim is to take users with this system within the next year.…”

Section: Technical Reportsmentioning

confidence: 99%

Synchrotron-Based Infrared Spectral Imaging at the MIRIAM Beamline of Diamond Light Source

Cinque

Frogley

Wehbe

et al. 2017

Synchrotron Radiation News

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Section: Technical Reportsmentioning

confidence: 99%

Synchrotron-Based Infrared Spectral Imaging at the MIRIAM Beamline of Diamond Light Source

Cinque

Frogley

Wehbe

et al. 2017

Synchrotron Radiation News

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“…Previous exciting works in adaptive optics (AO) have exploited these advantages and demonstrated aberration correction for a large field of view (FOV) by averaging the correction wavefront or using only the low-order modes of correction (5)(6)(7)(8)(9). However, these methods are valid when imaging transparent tissues or at shallow depth in turbid tissues.…”

mentioning

confidence: 99%

High-resolution in vivo imaging of mouse brain through the intact skull

Park

Sun

Cui

2015

Proc. Natl. Acad. Sci. U.S.A.

148

130

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Multiphoton microscopy is the current method of choice for in vivo deep-tissue imaging. The long laser wavelength suffers less scattering, and the 3D-confined excitation permits the use of scattered signal light. However, the imaging depth is still limited because of the complex refractive index distribution of biological tissue, which scrambles the incident light and destroys the optical focus needed for high resolution imaging. Here, we demonstrate a wavefrontshaping scheme that allows clear imaging through extremely turbid biological tissue, such as the skull, over an extended corrected field of view (FOV). The complex wavefront correction is obtained and directly conjugated to the turbid layer in a noninvasive manner. Using this technique, we demonstrate in vivo submicron-resolution imaging of neural dendrites and microglia dynamics through the intact skulls of adult mice. This is the first observation, to our knowledge, of dynamic morphological changes of microglia through the intact skull, allowing truly noninvasive studies of microglial immune activities free from external perturbations.neuroimaging | nonlinear microscopy | wavefront shaping | immunology | adaptive optics B reakthroughs in imaging technologies have been one of the major driving forces of new discoveries in biology (1). In the past two decades, we have witnessed that the advances of superresolution microscopy revolutionized our understanding of the complex dynamics in single cells (2). However, the techniques that work well on cultured cells often fail when being used to observe the cells in their native environment inside a living organism, the most ideal condition for biological studies. These difficulties result from the optical wavefront distortions induced by the inhomogeneous refractive index distribution in biological tissue.Because of this limitation, imaging inside deep tissue has been a challenging task, and the common goal of state of the art deep tissue-imaging methods is to recover the diffraction-limited resolution. These efforts can be categorized largely into two groups: the first capitalizes the use of longer wavelengths (3, 4) that undergo less scattering, whereas the second controls the optical wavefront, so that the wavefront distortions induced by the sample can be compensated (5-30). Longer wavelength excitation has demonstrated impressive penetration depths in the exposed brain (3) and imaging of the brain vascular structures beneath the skull (4). However, at the current state, using longer wavelengths is not a simple option because it requires special illumination sources [e.g., low repetition rate, high energy pulses (3)] or fluorescent probes (4), and provides only moderate spatial resolutions. In comparison, wavefront shaping allows the use of conventional light sources as well as the wide selection of wellestablished labels and functional indicators (31).Previous exciting works in adaptive optics (AO) have exploited these advantages and demonstrated aberration correction for a large field of view (FOV) by avera...

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“…We chose an approach to change the phase information from a hologram to compensate optical system wave aberrations in the viewing-window holographic display. The renowned approach to changing a phase is to use both a wavefront sensor (or an optical interferometer) that can quantitatively measure wave aberrations and a deformable mirror that controls a physical surface in a random form [18], [19].…”

Section: Introductionmentioning

confidence: 99%

Distortion Compensation of Reconstructed Hologram Image in Digital Holographic Display Based on Viewing Window

Park¹,

Kim²,

Choo³

et al. 2017

ETRI Journal

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A holographic display based on a viewing window enables the converging of a reconstruction wave into a viewing window by means of an optical system. Accordingly, a user can observe a reconstructed hologram image, even with a small diffraction angle. It is very difficult to manufacture an optical system with no aberrations; thus, it is inevitable that a certain amount of wave aberrations will exist. A viewing-window-based holographic display, therefore, always includes distortions in an image reconstructed from a hologram pattern. Compensating the distortions of a reconstructed image is a very important technical issue because it can dramatically improve the performance when reconstructing a digital three-dimensional content image from a hologram pattern. We therefore propose a method for suppressing image distortion by measuring and compensating the wave aberration calculated from a Zernike polynomial, which can represent arbitrary wave aberrations. Through our experimental configuration using only numerical calculations, our proposed method decreased the reconstructed image distortion by more than 28%.

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Adaptive optics in microscopy

Cited by 428 publications

References 92 publications

Synchrotron-Based Infrared Spectral Imaging at the MIRIAM Beamline of Diamond Light Source

Synchrotron-Based Infrared Spectral Imaging at the MIRIAM Beamline of Diamond Light Source

High-resolution in vivo imaging of mouse brain through the intact skull

Distortion Compensation of Reconstructed Hologram Image in Digital Holographic Display Based on Viewing Window

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