Synchrotron-Based Infrared Spectral Imaging at the MIRIAM Beamline of Diamond Light Source

Cinque, Gianfelice; Frogley, Mark D.; Wehbe, Katia; Nguyen, Tiffany; Fitzpatrick, Ann; Kelley, Chris S.

doi:10.1080/08940886.2017.1338416

Cited by 7 publications

(5 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SR-microFTIR measurements were performed at Diamond Light Source Synchrotron (Oxfordshire, UK) at the MIRIAM beamline B22 46 . A Bruker Vertex 80 V in vacuum FTIR with resolution 4 cm −1 was utilised.…”

Section: Methodsmentioning

confidence: 99%

Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Domenici

Capocefalo

Brasili

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Ultrasound (US) induced transient membrane permeabilisation has emerged as a hugely promising tool for the delivery of exogenous vectors through the cytoplasmic membrane, paving the way to the design of novel anticancer strategies by targeting functional nanomaterials to specific biological sites. An essential step towards this end is the detailed recognition of suitably marked nanoparticles in sonoporated cells and the investigation of the potential related biological effects. By taking advantage of Synchrotron Radiation Fourier Transform Infrared micro-spectroscopy (SR-microFTIR) in providing highly sensitive analysis at the single cell level, we studied the internalisation of a nanoprobe within fibroblasts (NIH-3T3) promoted by low-intensity US. To this aim we employed 20 nm gold nanoparticles conjugated with the IR marker 4-aminothiophenol. The significant Surface Enhanced Infrared Absorption provided by the nanoprobes, with an absorbance increase up to two orders of magnitude, allowed us to efficiently recognise their inclusion within cells. Notably, the selective and stable SR-microFTIR detection from single cells that have internalised the nanoprobe exhibited clear changes in both shape and intensity of the spectral profile, highlighting the occurrence of biological effects. Flow cytometry, immunofluorescence and murine cytokinesis-block micronucleus assays confirmed the presence of slight but significant cytotoxic and genotoxic events associated with the US-nanoprobe combined treatments. Our results can provide novel hints towards US and nanomedicine combined strategies for cell spectral imaging as well as drug delivery-based therapies.

show abstract

Section: Methodsmentioning

confidence: 99%

Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Domenici

Capocefalo

Brasili

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…An infrared objective lens of 36× FPA-FTIR microspectroscopy gave higher magnification and increased numerical aperture. The effective pixel size resolution of 36× on the sample gave much more detail than 15× and could classify small layers from rice tissue (Wehbe et al, 2015; Lloyd et al, 2016; Cinque et al, 2017; Kimber & Kazarian, 2017; Liberda et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

Performance Evaluation of Focal Plane Array (FPA)-FTIR and Synchrotron Radiation (SR)-FTIR Microspectroscopy to Classify Rice Components

Siriwong

Tanthanuch

Srisamut

et al. 2022

Microscopy and Microanalysis

View full text Add to dashboard Cite

The development of biochemical analysis techniques to study heterogeneous biological samples is increasing. These techniques include synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy. This method has been applied to analyze biological tissue with multivariate statistical analysis to classify the components revealed by the spectral data. This study aims to compare the efficiencies of SR-FTIR microspectroscopy and focal plane array (FPA)-FTIR microspectroscopy when classifying rice tissue components. Spectral data were acquired for mapping the same sample areas from both techniques. Principal component analysis and cluster imaging were used to investigate the biochemical variations of the tissue types. The classification was based on the functional groups of pectin, protein, and polysaccharide. Four layers from SR-FTIR microspectroscopy including pericarp, aleurone layer, sub-aleurone layer, and endosperm were classified using cluster imaging, while FPA-FTIR microspectroscopy could classify only three layers of pericarp, aleurone layer, and endosperm. Moreover, SR-FTIR microspectroscopy increased the image contrast of the biochemical distribution in rice tissue more efficiently than FPA-FTIR microspectroscopy. We have demonstrated the capability of the high-resolution synchrotron technique and its ability to clarify small structures in rice tissue. The use of this technique might increase in future studies of tissue characterization.

show abstract

“…Label-free spectroscopic imaging has been emerging as a central tool for elucidating the global chemical changes caused by infection with obligate intracellular protozoan parasites, such as T. gondii [ 4 , 9 ]. FTIR spectroscopy is an effective label-free imaging tool that can detect the (bio)molecular constituents of individual host cells without the use of fluorescent probes [ 27 ]. In the present study, we investigated the general dynamics of chemical changes that accompany T. gondii infection of hBMECs using conventional FTIR micro spectroscopic analysis, which revealed no significant differences between infected and control cells at 24 hpi or 48 hpi in PC1.…”

Section: Discussionmentioning

confidence: 99%

Illuminating Host-Parasite Interaction at the Cellular and Subcellular Levels with Infrared Microspectroscopy

Elsheikha

Al-sandaqchi

Harun

et al. 2022

Cells

Self Cite

View full text Add to dashboard Cite

Toxoplasma gondii (T. gondii) is an opportunistic protozoan that can cause brain infection and other serious health consequences in immuno-compromised individuals. This parasite has a remarkable ability to cross biological barriers and exploit the host cell microenvironment to support its own survival and growth. Recent advances in label-free spectroscopic imaging techniques have made it possible to study biological systems at a high spatial resolution. In this study, we used conventional Fourier-transform infrared (FTIR) microspectroscopy and synchrotron-based FTIR microspectroscopy to analyze the chemical changes that are associated with infection of human brain microvascular endothelial cells (hBMECs) by T. gondii (RH) tachyzoites. Both FTIR microspectroscopic methods showed utility in revealing the chemical alterations in the infected hBMECs. Using a ZnS hemisphere device, to increase the numerical aperture, and the synchrotron source to increase the brightness, we obtained spatially resolved spectra from within a single cell. The spectra extracted from the nucleus and cytosol containing the tachyzoites were clearly distinguished. RNA sequencing analysis of T. gondii-infected and uninfected hBMECs revealed significant changes in the expression of host cell genes and pathways in response to T. gondii infection. These FTIR spectroscopic and transcriptomic findings provide significant insight into the molecular changes that occur in hBMECs during T. gondii infection.

show abstract

Synchrotron-Based Infrared Spectral Imaging at the MIRIAM Beamline of Diamond Light Source

Cited by 7 publications

References 7 publications

Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Performance Evaluation of Focal Plane Array (FPA)-FTIR and Synchrotron Radiation (SR)-FTIR Microspectroscopy to Classify Rice Components

Illuminating Host-Parasite Interaction at the Cellular and Subcellular Levels with Infrared Microspectroscopy

Contact Info

Product

Resources

About