Adaptive Optics for Biological Imaging using Direct Wavefront Sensing

Kubby, Joel; Azucena, Oscar; Tao, Xiaodong

doi:10.1364/aopt.2013.om2a.4

Cited by 57 publications

(73 citation statements)

References 3 publications

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“…[2][3][4] Two main factors are enabling this transition: the invention of multiphoton microscopes, notably the two-photon microscope, 5,6 and adaptive optics. 7,8 While one-photon methods, especially confocal microscopy, have been and will continue to be immensely useful across a wide range of conditions, two-photon excited fluorescence (TPEF) microscopy is often the method of choice for imaging in tissue because it benefits from intrinsic optical sectioning, cellular resolution, high sensitivity, and high imaging rate. Importantly, it is resilient to image degradation from the light scattering in tissue thanks to the nonlinear dependence upon illumination intensity as well as the detection scheme, which singles out ballistic photons, which carry information about the sample and discriminates against scattered photons, which carry no spatial information.…”

Section: Challenges In Deep Tissue Imagingmentioning

confidence: 99%

Ultrathin endoscopes based on multicore fibers and adaptive optics: a status review and perspectives

et al. 2016

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Section: Challenges In Deep Tissue Imagingmentioning

confidence: 99%

Ultrathin endoscopes based on multicore fibers and adaptive optics: a status review and perspectives

et al. 2016

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“…3,4 Spatial light modulators have been shown to correct these aberrations and improve the image quality of biospecimens. 5,6 However, these reflective spatial light modulators require the user to change the basic design of an optical path in a conventional microscope.…”

Section: Introductionmentioning

confidence: 99%

Transmissive liquid-crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy

et al. 2016

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Nemoto, "Transmissive liquid-crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy," J. Abstract. All aberrations produced inside a biospecimen can degrade the quality of a three-dimensional image in two-photon excitation laser scanning microscopy. Previously, we developed a transmissive liquid-crystal device to correct spherical aberrations that improved the image quality of a fixed-mouse-brain slice treated with an optical clearing reagent. In this study, we developed a transmissive device that corrects primary coma aberration and astigmatism. The motivation for this study is that asymmetric aberration can be induced by the shape of a biospecimen and/or by a complicated refractive-index distribution in a sample; this can considerably degrade optical performance even near the sample surface. The device's performance was evaluated by observing fluorescence beads. The device was inserted between the objective lens and microscope revolver and succeeded in improving the spatial resolution and fluorescence signal of a bead image that was originally degraded by asymmetric aberration. Finally, we implemented the device for observing a fixed whole mouse brain with a sloping surface shape and complicated internal refractive-index distribution. The correction with the device improved the spatial resolution and increased the fluorescence signal by ∼2.4×. The device can provide a simple approach to acquiring higher-quality images of biospecimens.

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“…Adaptive optics (AO) has proven to be an effective strategy to counter such aberrations. 1,2 In its most common implementation, called pupil AO, a wavefront correcting device, typically a deformable mirror (DM), is placed in the rear focal plane of the objective lens. 3 This implementation works well when the wavefront aberrations are spatially, or shift, invariant, such as in the case of spherical aberrations.…”

Section: Introductionmentioning

confidence: 99%

Widefield fluorescence microscopy with sensor-based conjugate adaptive optics using oblique back illumination

Bifano

Mertz

2016

J. Biomed. Opt.

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fluorescence microscopy with sensor-based conjugate adaptive optics using oblique back illumination," J. Abstract. We describe a wavefront sensor strategy for the implementation of adaptive optics (AO) in microscope applications involving thick, scattering media. The strategy is based on the exploitation of multiple scattering to provide oblique back illumination of the wavefront-sensor focal plane, enabling a simple and direct measurement of the flux-density tilt angles caused by aberrations at this plane. Advantages of the sensor are that it provides a large measurement field of view (FOV) while requiring no guide star, making it particularly adapted to a type of AO called conjugate AO, which provides a large correction FOV in cases when sample-induced aberrations arise from a single dominant plane (e.g., the sample surface). We apply conjugate AO here to widefield (i.e., nonscanning) fluorescence microscopy for the first time and demonstrate dynamic wavefront correction in a closed-loop implementation.

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Adaptive Optics for Biological Imaging using Direct Wavefront Sensing

Cited by 57 publications

References 3 publications

Ultrathin endoscopes based on multicore fibers and adaptive optics: a status review and perspectives

Ultrathin endoscopes based on multicore fibers and adaptive optics: a status review and perspectives

Transmissive liquid-crystal device for correcting primary coma aberration and astigmatism in biospecimen in two-photon excitation laser scanning microscopy

Widefield fluorescence microscopy with sensor-based conjugate adaptive optics using oblique back illumination

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