“…IHT may provoke beneficial effects by preconditioning subsequently protecting the heart and/or the brain against deleterious consequences of ischemia reperfusion [13] . Although the excessive production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) represents an important mechanism of cell damage during hypoxia and reoxygenation in mitochondria initiating cellular death pathways, IHT may optimize mitochondrial metabolism, thus preventing adverse consequences of excess mitochondrial ROS generation [14] , [15] . In addition, IHT stimulates endothelial nitric oxide (NO) production that leads to vasodilatation, opens reserve capillaries [16] , and induces the production of vascular endothelial and fibroblast growth factors to stimulate endothelial proliferation [17] .…”
IntroductionIntermittent hypoxic–hyperoxic training (IHHT) may complement a multimodal training intervention (MTI) for improving cognitive function and exercise tolerance in geriatric patients.MethodsThirty-four patients (64–92 years) participated in this randomized controlled trial. Before and after the 5- to 7-week intervention period (MTI + IHHT vs. MTI + ambient air), cognitive function was assessed by the Dementia-Detection Test (DemTect) and the Sunderland Clock-Drawing Test (CDT), and functional exercise capacity by the total distance of the 6-Minute Walk Test (6MWT).ResultsDemTect and CDT indicated significantly larger improvements after MTI + IHHT (+16.7% vs. −0.39%, P < .001) and (+10.7% vs. −8%, P = .031) which was also true for the 6MWT (+24.1% vs. +10.8%, P = .021).DiscussionIHHT turned out to be easily applicable to and well tolerated by geriatric patients up to 92 years. IHHT contributed significantly to improvements in cognitive function and functional exercise capacity in geriatric patients performing MTI.
“…IHT may provoke beneficial effects by preconditioning subsequently protecting the heart and/or the brain against deleterious consequences of ischemia reperfusion [13] . Although the excessive production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) represents an important mechanism of cell damage during hypoxia and reoxygenation in mitochondria initiating cellular death pathways, IHT may optimize mitochondrial metabolism, thus preventing adverse consequences of excess mitochondrial ROS generation [14] , [15] . In addition, IHT stimulates endothelial nitric oxide (NO) production that leads to vasodilatation, opens reserve capillaries [16] , and induces the production of vascular endothelial and fibroblast growth factors to stimulate endothelial proliferation [17] .…”
IntroductionIntermittent hypoxic–hyperoxic training (IHHT) may complement a multimodal training intervention (MTI) for improving cognitive function and exercise tolerance in geriatric patients.MethodsThirty-four patients (64–92 years) participated in this randomized controlled trial. Before and after the 5- to 7-week intervention period (MTI + IHHT vs. MTI + ambient air), cognitive function was assessed by the Dementia-Detection Test (DemTect) and the Sunderland Clock-Drawing Test (CDT), and functional exercise capacity by the total distance of the 6-Minute Walk Test (6MWT).ResultsDemTect and CDT indicated significantly larger improvements after MTI + IHHT (+16.7% vs. −0.39%, P < .001) and (+10.7% vs. −8%, P = .031) which was also true for the 6MWT (+24.1% vs. +10.8%, P = .021).DiscussionIHHT turned out to be easily applicable to and well tolerated by geriatric patients up to 92 years. IHHT contributed significantly to improvements in cognitive function and functional exercise capacity in geriatric patients performing MTI.
“…Further, we hypothesize that the oxygen tensions and intermittent hyperoxia exposure-associated H3K4me3 alteration in our cell culture system may have been due to the increased production of reactive oxygen species (ROS). It is known that higher O 2 tension and re-oxygenation are both accompanied by increased generation of ROS [37,38]. Unlike the in vivo system, culture conditions in vitro lack naturally effective antioxidant systems [39].…”
Epigenetics play a vital role in early embryo development. Offspring conceived via assisted reproductive technologies (ARTs) have a three times higher risk of epigenetic diseases than naturally conceived children. However, investigations into ART-associated placental histone modifications or sex-stratified analyses of ART-associated histone modifications remain limited. In the current study, we carried out immunohistochemistry, chip-sequence analysis, and a series of in vitro experiments. Our results demonstrated that placentas from intra-cytoplasmic sperm injection (ICSI), but not in vitro fertilization (IVF), showed global tri-methylated-histone-H3-lysine-4 (H3K4me3) alteration compared to those from natural conception. However, for acetylated-histone-H3-lysine-9 (H3K9ac) and acetylated-histone-H3-lysine-27 (H3K27ac), no significant differences between groups could be found. Further, sex -stratified analysis found that, compared with the same-gender newborn cord blood mononuclear cell (CBMC) from natural conceptions, CBMC from ICSI-boys presented more genes with differentially enriched H3K4me3 (n = 198) than those from ICSI-girls (n = 79), IVF-girls (n = 5), and IVF-boys (n = 2). We also found that varying oxygen conditions, RNA polymerase II subunit A (Polr2A), and lysine demethylase 5A (KDM5A) regulated H3K4me3. These findings revealed a difference between IVF and ICSI and a difference between boys and girls in H3K4me3 modification, providing greater insight into ART-associated epigenetic alteration.
“…As outlined above, mechanisms responsible for hypoxia effects may be either related or unrelated to hypoxia-induced HIF activation as recently reviewed [69], and include improvements of stress resistance on the cellular and systemic level [27,81], glucose homeostasis [41,61] and blood lipid profile [40,64,110], as well as the evocation of antiarrhythmic effects and improved autonomic cardiovascular and respiratory control [27,59], and neuroprotection by upregulating neuroprotectants like VEGF, EPO, antioxidants and nitric oxide (NO), and/or by suppressing apoptosis [66]. Regarding IHHC, reoxygenation, especially when performed under hyperoxic conditions, generates ROS, which may trigger redox-signaling cascades initiating adaptations that contribute to injury resistance, e.g., by membrane-stabilizing effects in the heart, brain, and liver [3,105,106,113]. Although the multitude of signaling pathways involved in hypoxia adaptations and their interactions are far from being fully elucidated, they may not only be used for the benefit of different patient groups but also contribute to (pre-)acclimatization, improved exercise tolerance and training efficiency (demonstrated by Sazontova and colleagues [105,106].…”
Section: Intermittent Hypoxia and Hypoxia-hyperoxia Conditioning In Therapy And The Prevention Of Various Diseasesmentioning
Purpose
Main purposes of pre-acclimatization by hypoxia conditioning (HC) are the prevention of high-altitude illnesses and maintenance of aerobic exercise performance. However, robust evidence for those effects or evidence-based guidelines for exposure strategies, including recommendations to ensure safety, are largely lacking. Therefore, we summarize the current knowledge on the physiology of acclimatization to hypoxia and HC with the aim to derive implications for pre-acclimatization strategies before going on high-altitude treks and expeditions.
Methods
Based on the literature search and personal experience, core studies and important observations have been selected in order to present a balanced view on the current knowledge of high-altitude illnesses and the acclimatization process, specifically focusing on pre-acclimatization strategies by HC.
Results and Conclusions
It may be concluded that in certain cases even short periods (e.g., 7 h) of pre-acclimatization by HC are effective, but longer periods (e.g., > 60 h) are needed to elicit more robust effects. About 300 h of HC (intermittently applied) may be the optimal preparation for extreme altitude sojourns, although every additional hour spent in hypoxia may confer further benefits. The inclusion of hypobaric exposures (i.e., real altitude) in pre-acclimatization protocols could further increase their efficacy. The level of simulated altitude is progressively increased or individually adjusted ideally. HC should not be terminated earlier than 1–2 weeks before altitude sojourn. Medical monitoring of the pre-acclimatization program is strongly recommended.
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