Adaptation of the Phosphotungstate Method to Determine Reduced and Oxidized Vitamin C in Blood Plasma

Rutkowski, Maciej; Grzegorczyk, Krzysztof; Greger, Janusz

doi:10.1515/znc-2004-9-1023

Cited by 9 publications

(9 citation statements)

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“…3 In all patients, the determinations of the plasma level of vitamin A, C and E were performed by spectrophotometric method using a spectrophotometer T60V (PG Instruments, Leicestershire, UK) according to the modified Rutkowski et al method. [6][7][8] Plasma levels of the investigated vitamins were given in µmol/L. Plasma vitamin deficiency was stated for vitamin A < 0.9 μmol/L, for vitamin C < 36.1 μmol/L and for vitamin E < 12 μmol/L.…”

Section: Biochemical Analysesmentioning

confidence: 99%

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The risk of plasma vitamin A, C, E and D deficiency in patients with metabolic syndrome: a case-control study

Godala¹,

Materek-Kuśmierkiewicz²,

Moczulski³

et al. 2017

Adv Clin Exp Med

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Background. The increasing incidence of metabolic diseases such as obesity or diabetes have made them a major public health problem. Increasing oxidative stress induced by reactive oxygen species, which initiate the oxidative adverse changes in the cell, is mentioned, among other risk factors, to underlie these diseases. Vitamin A, C and E are listed among the non-enzymatic mechanisms counteracting this phenomenon. Vitamin D deficiency is also associated with cardiovascular diseases.

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Section: Biochemical Analysesmentioning

confidence: 99%

“…Plasma vitamin deficiency was stated for vitamin A < 0.9 μmol/L, for vitamin C < 36.1 μmol/L and for vitamin E < 12 μmol/L. [6][7][8] …”

Section: Biochemical Analysesmentioning

confidence: 99%

The risk of plasma vitamin A, C, E and D deficiency in patients with metabolic syndrome: a case-control study

Godala¹,

Materek-Kuśmierkiewicz²,

Moczulski³

et al. 2017

Adv Clin Exp Med

Self Cite

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“…In all patients, the determinations of the serum level of vitamin E, A and C were performed by spectrophotometric method using a spectrophotometer T60V (PG Instruments) according to the modified Rutkowski et al method [8][9][10][11]. Plasma levels of the investigated vitamins were given in µmol/L.…”

Section: Biochemical Analysesmentioning

confidence: 99%

“…Plasma vitamin deficiency was stated for vitamin A < 0.9 μmol/L, for vitamin C < 36.1 μmol/L and for vitamin E < 12 μmol/L [8][9][10][11].…”

Section: Biochemical Analysesmentioning

confidence: 99%

Lower Plasma Levels of Antioxidant Vitamins in Patients with Metabolic Syndrome: A Case Control Study

Godala¹,

Materek-Kuśmierkiewicz²,

Moczulski³

et al. 2016

Adv Clin Exp Med

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blood pressure or hypertension treatment, fasting hyperglycemia or diagnosed type 2 diabetes, abnormal lipid profile (hypertriglyceridemia and/or low HDL-cholesterol) A -research concept and design; B -collection and/or assembly of data; C -data analysis and interpretation; D -writing the article; E -critical revision of the article; F -final approval of article AbstractBackground. Metabolic syndrome (MS) is a coexistence of metabolic risk factors affecting the development of cardiovascular diseases. Reactive oxygen species, which are excessively produced in MS, participate in its pathogenesis. Vitamins A, C and E are an important part of the non-enzymatic antioxidative barrier in humans. Objectives. The aim of the study was to estimate plasma vitamin A, C and E levels and the intake of these vitamins from the diet in patients with MS. Material and Methods. The study included 182 patients with MS, 94 men and 88 women, aged 30-65 years (mean 57.31 ± 8.28 years). The control group was comprised of 91 subjects, 56 men and 35 women, aged 41-65 years (mean 57.75 ± 5.84 years). The MS diagnosis was based on IDF criteria. The determination of the serum level of vitamin A, C and E was performed using the spectrophotometric method. The food intake was assessed by 24-h dietary recall. Results. The mean plasma vitamin A, C and E levels were significantly lower in MS patients than in the controls (p = 0.05). No correlation was found between vitamin A, C and E intake from the diet and their plasma concentrations in MS patients. Plasma vitamin A, C and E deficiency was observed significantly more often in MS patients than in the control group (15.38% vs. 2.19%, 79.12% vs. 8.79% and 60.45% vs. 5.49%, p < 0.0001, respectively). BMI was the one factor significantly affecting the mean value of vitamin A, C and E levels in MS patients. Conclusions. MS patients demonstrated significantly lower plasma levels of vitamin A, C and E compared to the healthy subjects. Lower plasma levels of antioxidant vitamins with their high intake from the diet indicate antioxidant barrier impairment in MS patients (Adv Clin Exp Med 2016, 25, 4, 689-700).

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“…It is unclear, however, if this dehydroascorbate transport plays a physiological role. Under physiological conditions, dehydroascorbate is found in blood only in small amounts compared to ascorbic acid [29], [30]. Since GLUTs are glucose transporters, dehydroascorbate competes with glucose for transport via GLUTs [31], [32].…”

Section: Discussionmentioning

confidence: 99%

Sodium-Dependent Vitamin C Transporter 2 (SVCT2) Expression and Activity in Brain Capillary Endothelial Cells after Transient Ischemia in Mice

Gess¹,

Sevimli²,

Strecker³

et al. 2011

PLoS ONE

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Expression and transport activity of Sodium-dependent Vitamin C Transporter 2 (SVCT2) was shown in various tissues and organs. Vitamin C was shown to be cerebroprotective in several animal models of stroke. Data on expression, localization and transport activity of SVCT2 after cerebral ischemia, however, has been scarce so far. Thus, we studied the expression of SVCT2 after middle cerebral artery occlusion (MCAO) in mice by immunohistochemistry. We found an upregulation of SVCT2 after stroke. Co-stainings with Occludin, Von-Willebrand Factor and CD34 demonstrated localization of SVCT2 in brain capillary endothelial cells in the ischemic area after stroke. Time-course analyses of SVCT2 expression by immunohistochemistry and western blots showed upregulation in the subacute phase of 2–5 days. Radioactive uptake assays using 14C-labelled ascorbic acid showed a significant increase of ascorbic acid uptake into the brain after stroke. Taken together, these results provide evidence for the expression and transport activity of SVCT2 in brain capillary endothelial cells after transient ischemia in mice. These results may lead to the development of novel neuroprotective strategies in stroke therapy.

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Adaptation of the Phosphotungstate Method to Determine Reduced and Oxidized Vitamin C in Blood Plasma

Cited by 9 publications

References 0 publications

The risk of plasma vitamin A, C, E and D deficiency in patients with metabolic syndrome: a case-control study

The risk of plasma vitamin A, C, E and D deficiency in patients with metabolic syndrome: a case-control study

Lower Plasma Levels of Antioxidant Vitamins in Patients with Metabolic Syndrome: A Case Control Study

Sodium-Dependent Vitamin C Transporter 2 (SVCT2) Expression and Activity in Brain Capillary Endothelial Cells after Transient Ischemia in Mice

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