Adaptation of the liquid chromatograph as a micro‐reactor; immobilised lactase kinetics

Melton, H. R.; Bailey, David C.; Langer, Stanley H.

doi:10.1002/jctb.503310108

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…One of the primary industrial interests in immobilized enzyme microreactor technology is the speedy design of industrially relevant bioprocesses as a means of overcoming known bottlenecks in the overall process development scheme. Thus, recent efforts have started to attract industrial interest, such as the application of immobilized lactases for the enzymatic hydrolysis of lactose from cheese whey122 or the monolith‐immobilized enzymatic production of bioactive oligosaccharides for industrial scale‐up applications 91…”

Section: Synthesismentioning

confidence: 99%

Fundamentals and applications of immobilized microfluidic enzymatic reactors

Matosevic

Szita

Baganz

2011

J of Chemical Tech & Biotech

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OVERVIEW: Over the last decade, the utility of immobilized microfluidic enzyme reactors (IMERs) has been demonstrated in a wide variety of fields, including medical diagnostics and therapy, biosensors, organic synthesis, drug discovery and many other applications. Of particular interest to the pharmaceutical industry is the potential for high throughput experimentation afforded by these systems, with a view to combinatorial synthesis for drug discovery applications. This article will focus on the current state of IMER systems, including immobilization techniques and microchannel flow generation, with a particular emphasis on applications and future prospects in view of likely directions and market potential of this field.IMPACT: The numerous advantages of attaching enzymes to a solid support, such as reuse of a single batch of enzyme, improved stability and durability, the ability to stop the reaction rapidly by removing the product from the reaction solution and the absence of enzyme contamination of the product are some of the attractive features of such systems. There are, however, a number of issues requiring careful consideration when developing such microsystems, including, but not limited to, surface modifications and exact control of fluid behaviour in microchannels, detection limitations, increased integration, and the reusability of the chips. APPLICATIONS: IMERs have received wide, including commercial, application as diagnostic tools for point-of-care applications, and, increasingly, as analytical tools in early drug development. Furthermore, peptide mapping and proteomics have employed IMER systems extensively over the past decade and growth in these areas continues.

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Section: Synthesismentioning

confidence: 99%

Fundamentals and applications of immobilized microfluidic enzymatic reactors

Matosevic

Szita

Baganz

2011

J of Chemical Tech & Biotech

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Chromatographic Reactors

Fricke¹,

Schmidt–Traub²,

Schembecker³

2008

Ullmann's Encyclopedia of Industrial Chemistry

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Chromatographic reactors integrate chemical reaction and chromatographic separation in one apparatus. This offers potential for process intensification, especially in the case of equilibrium reactions. Different types of discontinuous and continuous processes as well as modeling of chromatographic reactors are described. Synthesis and design of this processes is very much influenced and often restricted by the type of reaction and the operating window which is set by the individual operating conditions for chemical reaction, mass separation, and equipment design. Integrated chromatographic reactors should be considered if chromatography is the favored separation process for a conventional sequential process design. Preparative chromatographic reactors are known for liquid‐ and gas‐phase processes. Laboratory‐scale applications are described. Analytical chromatographic reactors are used to determine reaction rate constants and kinetics, and to screen chromatographic systems and operating conditions for preparative applications. 1. Introduction 2. Types of Chromatographic Reactors 3. Modeling of Chromatographic Reactors 3.1. Adsorption Isotherm and Reaction Kinetics 3.2. Modeling the BCR 3.3. Modeling Simulated Moving Bed Chromatographic Reactors 3.4. Process control 4. Synthesis of Preparative Chromatographic Reactors 4.1. Process Principles 4.2. Type of Reaction 5. Design of Preparative Chromatographic Reactors 5.1. Design of the Process 5.2. Choice of Operating Conditions 6. Preparative Chromatographic Reactors 6.1. Applications of Gas Chromatographic Reactors 6.2. Application of Liquid Chromatographic Reactors 7. Analytical Chromatographic Reactors 7.1. Determination of Rate Constants 7.2. Enzymatic Reactions 7.3. Characterization of the Stationary Phase 7.4. Examination of Fast Equilibrium Reactions 7.5. Examination of Solvent Effects

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