Adaptation of Glucose-grown Saccharomyces cerevisiae to Gluconeogenic Growth and Sporulation

Chu, Martha I.; Hartig, Andreas; Freese, Ernst

doi:10.1099/00221287-125-2-421

Cited by 4 publications

(7 citation statements)

References 20 publications

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“…Bjorling and Lindman (1989) reported complete inhibition of ethanol production by P. stipitis in a medium containing 3á9 g l )1 acetic acid. The pH-dependent inhibition of growth and ethanol production has been described previously (Chu et al 1981). Under acidic conditions, there is a decline in the cellular ATP pool which affects the ability of the cells to synthesize the necessary apparatus of respiratory metabolism and culture recovery, and this phenomenon may be working in the experiments described here.…”

Section: Parent Culturementioning

confidence: 62%

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Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Nigam

2001

J Appl Microbiol

118

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Aims: The objective of this study was to develop a mutant from Pichia stipitis NRRL Y-7124, tolerant of high concentrations of acetic acid and other inhibitory components present in acid hydrolysates, to improve ethanol yield and productivity. Methods and Results: The mutant was developed through adaptation in acid hydrolysate supplemented with nutrients and minerals at 30 0á5°C. When it was tested for its ability to ferment acid hydrolysate, it showed shorter fermentation time, better tolerance to acid and could ferment at lower pH. The ethanol yield (Y p/s ) and productivity (Q p ) were increased 1á6-and 2á1-fold, respectively. Conclusions: The development of a mutant and its tolerance to acetic acid present in hydrolysates is described. The selected mutant is capable of fermenting both hexoses and pentoses present in hydrolysate at lower pH in comparison with the parent strain. Signi®cance and Impact of the Study: The mutant could play a signi®cant role in reducing environmental pollution by using sugars present in pulp mill ef¯uent and, at the same time, could produce a marketable liquid fuel ethanol.

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Section: Parent Culturementioning

confidence: 62%

“…Bjorling and Lindman (1989) reported complete inhibition of ethanol production by P. stipitis in a medium containing 3·9 g l −1 acetic acid. The pH‐dependent inhibition of growth and ethanol production has been described previously (Chu et al . 1981).…”

Section: Discussionmentioning

confidence: 99%

Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Nigam

2001

J Appl Microbiol

118

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“…Whereas cells growing exponentially in glucose medium can adapt readily to grow in ethanol medium, they cannot do so after transfer to acetate medium (2). It is also known that yeast growing exponentially in glucose-containing media sporulate poorly and do so only several days after transfer to potassium acetate (4,7,29).…”

Section: Discussionmentioning

confidence: 99%

“…It is also known that yeast growing exponentially in glucose-containing media sporulate poorly and do so only several days after transfer to potassium acetate (4,7,29). The much slower adaptation to acetate than to ethanol has been attributed to the lack of metaboliza-ble electron donors (NADH) after glucose removal, causing a deficiency of ATP in acetate medium (2). To obtain good sporulation on potassium acetate as a sporulation medium, either cells have to be grown in glucose medium until all of the glucose is used up (4, 7) and until they have adapted to ethanol, or they have to be grown in an acetate-containing (29) or ethanolcontaining glucose-free medium.…”

Section: Discussionmentioning

confidence: 99%

“…Glycogen accumulates only after sporulation has been initiated by cell transfer to acetate; eventually, the acetate can be removed, and glycogen be used as a carbon source (15,16). A carbon source is needed to maintain ATP (2) and to allow the synthesis of new macromolecules needed for meiosis and sporulation; some new proteins made during sporulation on potassium acetate have been identified (18,20). Other necessary precursors, including nitrogenand phosphate-containing compounds, are usually available inside the cells during sporulation on acetate.…”

mentioning

confidence: 99%

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Initiation of Yeast Sporulation by Partial Carbon, Nitrogen, or Phosphate Deprivation

Chu

Freese

1982

J Bacteriol

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In this paper we show that partial deprivation of a carbon source, a nitrogen source, or phosphate in the presence of all other nutrients needed for growth initiates meiosis and sporulation of Saccharomyces cerevisiae homothallic strain Y55. For carbon deprivation experiments, cells were grown in synthetic medium (pH 5.5) containing an excess of one carbon source and then transferred to the same medium containing different concentrations of the same carbon source. In the case of transfer to different acetate concentrations, the log optical density at 600 nm increased at the previous rate until the cells had used up all of the acetate, whereupon the cells entered a stationary phase and did not sporulate. The same was observed with ethanol. In contrast, at different concentrations of dihydroxy-acetone or pyruvate, cells grew at different rates and sporulated optimally at intermediate concentrations (50 to 75 mM). The response to galactose was similar but reflected the presence of a low-affinity galactose transport system and the induction of a high-affinity galactose transport system. Cells could also sporulate when a glucose medium ran out of glucose, apparently because they initiated sporulation during the subsequent lag period and then used the produced ethanol as a carbon source. For phosphate deprivation experiments, cells growing with excess ethanol or pyruvate and phosphate were transferred to the same medium containing limiting amounts of phosphate. First, they used up the intracellular phosphate reserves for rapid growth, and then they sporulated optimally when an intermediate concentration (30 μM) of phosphate had been added to the medium. For nitrogen deprivation experiments, cells grown with excess acetate, ethanol, or pyruvate and NH 4 + were transferred to the same medium from which all nitrogen had been removed. These cells sporulated well in acetate medium but poorly in ethanol and pyruvate media. However, the sporulation frequency in the latter media could be increased greatly by adding intermediate concentrations (1 mM) of the slowly metabolizable amino acids glycine, histidine, or phenylalanine. If one assumes that the sporulation response to partial deprivation of carbon-, nitrogen-, or phosphorus-containing compounds reflects control by a single metabolite, the intracellular concentration of this metabolite may decide at the START position (G1 phase) of the cell cycle whether a /α cells enter mitosis or meiosis.

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Histidine is essential for growth of Komagataella phaffii cultured in YPA medium

Gupta

Rangarajan

2022

FEBS Open Bio

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Komagataella phaffii (a.k.a. Pichia pastoris) requires histidine for optimal growth when cultured in a medium containing yeast extract, peptone (YP), and acetate (YPA). We demonstrate that HIS4‐deficient, K. phaffii strain GS115 exhibits a growth defect on YP‐media containing acetate, but not on other carbon sources. K. phaffii X33, a prototroph, grows better than K. phaffii GS115 (his4), a histidine auxotroph in YPA. Normal growth of GS115 is restored either by the expression of HIS4 or by culturing in YPA containing ≥0.6 mM histidine. In the presence of histidine, expression of several genes is altered, including those encoding key subunits of mitochondrial ATP synthase, transporters of amino acids and nutrients, as well as biosynthetic enzymes. Thus, histidine should be included as an essential component for optimal growth of K. phaffii histidine auxotrophs cultured in YPA.

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Adaptation of Glucose-grown Saccharomyces cerevisiae to Gluconeogenic Growth and Sporulation

Cited by 4 publications

References 20 publications

Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Initiation of Yeast Sporulation by Partial Carbon, Nitrogen, or Phosphate Deprivation

Histidine is essential for growth of Komagataella phaffii cultured in YPA medium

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