Adaptation of a simple method to determine the total terpenoid content in needles of coniferous trees

Łukowski, Adrian; Jagiełło, Radosław; Robakowski, Piotr; Adamczyk, Dawid; Karolewski, Piotr

doi:10.1016/j.plantsci.2021.111090

Cited by 15 publications

(4 citation statements)

References 57 publications

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“…The determination of the total terpenoid content was carried out according to Lukowski et al’s methodology with some modifications [ 21 ]. First, 200 μL of diluted blackberry extract in methanol (50 mg/mL), methanol (blank) or standard (10–0.1 mg/mL geraniol) were added to 1.5 mL of chloroform.…”

Section: Methodsmentioning

confidence: 99%

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Gil-Martínez

Mut-Salud

Ruiz-García

et al. 2023

Foods

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A comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds in the extract were ursane-type terpenoids which, along with phenolic compounds, may be responsible for the bioactivity of the extract. In vitro antioxidant capacity was assessed through Folin–Ciocalteu (31.05 ± 4.9 mg GAE/g d.w.), FRAP (637.8 ± 3.2 μmol Fe2+/g d.w.), DPPH (IC50 97.1 ± 2.4 μg d.w./mL) and TEAC (576.6 ± 8.3 μmol TE/g d.w.) assays. Furthermore, the extract exerted remarkable effects on in vitro cellular antioxidant activity in HUVEC cells at a concentration of 5 mg/mL. Antimicrobial activity of the extract was also tested. Most sensible microorganisms were Gram-positive bacteria, such as E. faecalis, B. cereus and Gram-negative E. coli (MBC of 12.5 mg/mL). IC50 values against colon tumoral cells HT-29 (4.9 ± 0.2 mg/mL), T-84 (5.9 ± 0.3 mg/mL) and SW-837 (5.9 ± 0.2 mg/mL) were also obtained. Furthermore, blackberry extract demonstrated anti-inflammatory activity inhibiting the secretion of pro-inflammatory IL-8 cytokines in two cellular models (HT-29 and T-84) in a concentration-dependent manner. These results support that blackberry fruits are an interesting source of bioactive compounds that may be useful in the prevention and treatment of different diseases, mainly related to oxidative stress.

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Section: Methodsmentioning

confidence: 99%

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Gil-Martínez

Mut-Salud

Ruiz-García

et al. 2023

Foods

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“…Subsequently, MeOH 95% was added to the precipitate, and absorption was assessed using a spectrophotometer at 538 nm (GBC, Cintra 6, Australia). Menthol (C 10 H 20 O) was used as the standard 75 .…”

Section: Methodsmentioning

confidence: 99%

Optimization of the fermentation media, mathematical modeling, and enhancement of paclitaxel production by Alternaria alternata after elicitation with pectin

Rezazadeh,

Ghanati,

Bonfill

et al. 2024

Sci Rep

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Alternaria alternata fungus is a potent paclitaxel producer isolated from Corylus avellana. The major challenge is the lack of optimized media for endophytic fungi productivity. In the effort to maximize the production of taxoids by A. alternata, several fermentation conditions, including pH (pH 4.0–7.0), different types and concentrations of carbon (fructose, glucose, sucrose, mannitol, sorbitol, and malt extract), and nitrogen (urea, ammonium nitrate, potassium nitrate, ammonium phosphate, and ammonium sulfate) were applied step by step. Based on the results, A. alternata in a medium containing sucrose 5% (w/v) and ammonium phosphate 2.5 mM at pH 6.0 showed a rapid and sustainable growth rate, the highest paclitaxel yield (94.8 µg gFW−1 vs 2.8 µg gFW−1 in controls), and the maximum content of amino acids. Additionally, the effect of pectin was evaluated on fungus, and mycelia harvested. Pectin significantly enhanced the growth and taxoid yield on day 21 (respectively 171% and 116% of their corresponding on day 7). The results were checked out by mathematical modeling as well. Accordingly, these findings suggest a low-cost, eco-friendly, and easy-to-produce approach with excellent biotechnological potential for the industrial manufacture of taxoids.

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“…This paper shows the data on the amount of terpene loss from the infused pre-rolled cones by comparing the amount of FCT infused initially and after 7 days' storage. Estimation of FCT content was carried out based on the formed reddish brown precipitate after employing the measurement procedure by Ghorai et al [ 6 , 7 ]; the formed precipitate is shown in Fig. 1a .…”

Section: Data Descriptionmentioning

confidence: 99%

Data on terpene content in pre-rolled cone paper infused with terpene-containing flavours based on the formation of a partially soluble precipitate

Karangan,

Rahardjo,

Widagdo

et al. 2024

Data in Brief

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Adaptation of a simple method to determine the total terpenoid content in needles of coniferous trees

Cited by 15 publications

References 57 publications

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits

Optimization of the fermentation media, mathematical modeling, and enhancement of paclitaxel production by Alternaria alternata after elicitation with pectin

Data on terpene content in pre-rolled cone paper infused with terpene-containing flavours based on the formation of a partially soluble precipitate

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