Adaptation of a Methanogenic Consortium to Arsenite Inhibition

Rodríguez-Freire, Lucía; Moore, Sarah; Sierra-Álvarez, Reyes; Field, James A.

doi:10.1007/s11270-015-2672-3

Cited by 5 publications

(2 citation statements)

References 25 publications

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“…The IC 50 value calculated for both As(III) and As(V) in this bioassay was about 0.02 mM. The inhibition results obtained for As (III) are in close agreement with those reported in a previous study using a similar experimental protocol (Rodriguez-Freire et al, 2015). On the other hand, the acetoclastic methanogens in the inoculum utilized in this study were more sensitive to As(V) than previously reported by Sierra-Alvarez and coworkers for a methanogenic mixed culture (IC 50 > 0.50 mM) (Sierra-Alvarez et al, 2004).…”

Section: Microtox Acute Toxicity Bioassaysupporting

confidence: 91%

Ecotoxicity assessment of ionic As(III), As(V), In(III) and Ga(III) species potentially released from novel III-V semiconductor materials

Zeng

Gonzalez-Alvarez

Orenstein

et al. 2017

Ecotoxicology and Environmental Safety

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III-V materials such as indium arsenide (InAs) and gallium arsenide (GaAs) are increasingly used in electronic and photovoltaic devices. The extensive application of these materials may lead to release of III-V ionic species during semiconductor manufacturing or disposal of decommissioned devices into the environment. Although arsenic is recognized as an important contaminant due to its high toxicity, there is a lack of information about the toxic effects of indium and gallium ions. In this study, acute toxicity of As(III), As(V), In(III) and Ga(III) species was evaluated using two microbial assays testing for methanogenic activity and O uptake, as well as two bioassays targeting aquatic organisms, including the marine bacterium Aliivibrio fischeri (bioluminescence inhibition) and the crustacean Daphnia magna (mortality). The most noteworthy finding was that the toxicity is mostly impacted by the element tested. Secondarily, the toxicity of these species also depended on the bioassay target. In(III) and Ga(III) were not or only mildly toxic in the experiments. D. magna was the most sensitive organism for In(III) and Ga(III) with 50% lethal concentrations of 0.5 and 3.4mM, respectively. On the other hand, As(III) and As(V) caused clear inhibitory effects, particularly in the methanogenic toxicity bioassay. The 50% inhibitory concentrations of both arsenic species towards methanogens were about 0.02mM, which is lower than the regulated maximum allowable daily effluent discharge concentration (2.09mg/L or 0.03mM) for facilities manufacturing electronic components in the US. Overall, the results indicate that the ecotoxicity of In(III) and Ga(III) is much lower than that of the As species tested. This finding is important in filling the knowledge gap regarding the ecotoxicology of In and Ga.

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Section: Microtox Acute Toxicity Bioassaysupporting

confidence: 91%

Ecotoxicity assessment of ionic As(III), As(V), In(III) and Ga(III) species potentially released from novel III-V semiconductor materials

Zeng

Gonzalez-Alvarez

Orenstein

et al. 2017

Ecotoxicology and Environmental Safety

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“…Although a high degree of correlation does not confirm causality, these findings are in agreement with the published literature. Arsenic can be highly inhibitory, depending on its form, with trivalent forms having 50% inhibitory concentrations of 0.7 and 1.1 mg L −1 ; nonetheless, there have been few studies on the effect of arsenic on AD [146,147] and especially seaweed. However, the highest concentration of total arsenic in the reactors for this study was 0.6 mg L −1 below the 50% inhibitory concentration of the trivalent form of arsenic, and thus, the levels of arsenic, although potentially concerning, may not be inhibitory for methane production.…”

Section: Methane Potential Of S Natans VIII S Natans I S Fluitanmentioning

confidence: 99%

Sargassum Inundations in Turks and Caicos: Methane Potential and Proximate, Ultimate, Lipid, Amino Acid, Metal and Metalloid Analyses

et al. 2020

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The Caribbean has been experiencing beach inundations of pelagic Sargassum, causing environmental, health and financial issues. This study showed variations in the composition and methane potential (MP) between the species of Sargassum. The MPs for S. natans VIII, S. natans I and S. fluitans (145, 66 and 113 mL CH4 g−1 Volatile Solids) were considerably below theoretical potentials, possibly due to the high levels of indigestible fibre and inhibitors. The mixed mats Sargassum composition was substantially different from the individual species, being higher in ash, calcium, iron, arsenic and phenolics. The mixed mats produced no methane, perhaps due to the high levels of phenolics. There was a strong correlation between MP and phenolic content. Heavy metals and metalloids were at levels that should not cause concern, except for arsenic (21–124 mg kg−1 dry weight). Further work on the speciation of arsenic in Sargassum is required to fully determine the risk to health and agriculture. Both protein and lipid levels were low. The ‘indispensable amino acid’ profile compares favourably with that recommended by the World Health Organisation. Lipids had a high proportion of Polyunsaturated Fatty Acids. The use of Sargassum for biogas production could be challenging, and further work is required.

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The alkaloid gramine in the anaerobic digestion process—inhibition and adaptation of the methanogenic community

Popp

Harms

Sträuber

2016

Appl Microbiol Biotechnol

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As many plant secondary metabolites have antimicrobial activity, microorganisms of the anaerobic digestion process might be affected when plant material rich in these compounds is digested. Hitherto, the effects of plant secondary metabolites on the anaerobic digestion process are poorly investigated. In this study, the alkaloid gramine, a constituent of reed canary grass, was added daily to a continuous co-digestion of grass silage and cow manure. A transient decrease of the methane yield by 17 % and a subsequent recovery was observed, but no effect on other process parameters. When gramine was infrequently spiked in higher amounts, the observed inhibitory effect was even more pronounced including a 53 % decrease of the methane yield and an increase of acetic acid concentrations up to 96 mM. However, the process recovered and the process parameters were finally at initial values (methane yield around 255 LN CH4 per gram volatile solids of substrate and acetic acid concentration lower than 2 mM). The bacterial communities of the reactors remained stable upon gramine addition. In contrast, the methanogenic community changed from a well-balanced mixture of five phylotypes towards a strong dominance of Methanosarcina (more than two thirds of the methanogenic community) while Methanosaeta disappeared. Batch inhibition assays revealed that acetic acid was only converted to methane via acetoclastic methanogenesis which was more strongly affected by gramine than hydrogenotrophic methanogenesis and acetogenesis. Hence, when acetoclastic methanogenesis is the dominant pathway, a shift of the methanogenic community is necessary to digest gramine-rich plant material.

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Adaptation of a Methanogenic Consortium to Arsenite Inhibition

Cited by 5 publications

References 25 publications

Ecotoxicity assessment of ionic As(III), As(V), In(III) and Ga(III) species potentially released from novel III-V semiconductor materials

Ecotoxicity assessment of ionic As(III), As(V), In(III) and Ga(III) species potentially released from novel III-V semiconductor materials

Sargassum Inundations in Turks and Caicos: Methane Potential and Proximate, Ultimate, Lipid, Amino Acid, Metal and Metalloid Analyses

The alkaloid gramine in the anaerobic digestion process—inhibition and adaptation of the methanogenic community

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