Acylated Ascorbate Stimulates Collagen Synthesis in Cultured Human Foreskin Fibroblasts at Lower Doses than does Ascorbic Acid

Abstract

Acylated derivatives of ascorbic acid were found to be active in a number of biochemical and physiological processes. In the present study we investigated the effects of 6-O-palmitoyl ascorbate on collagen synthesis by cultured foreskin human fibroblasts. Our observations indicate a marked stimulatory effect on collagen synthesis by 6-O-palmitoyl ascorbate in the concentration range of 5-20 microM, while the synthesis stimulated by ascorbic acid was maximal at concentrations of 20-100 microM. Cells treated with 10 microM palmitoyl ascorbate for 36 h exhibited a production of collagen threefold greater than those in the presence of 10 microM ascorbic acid, and it was about the same as in cells treated with 100 microM ascorbic acid. By 48 h differences were not significant. Acylated ascorbate impaired vitality of the treated fibroblasts at concentrations exceeding 20 microM in media supplemented with 0.5% FCS. However, most of the cytotoxic effect was neutralized by FCS at a concentration of 10%. The resistance of acylated ascorbate against oxidative degradation as well as the role of free radicals in the modulation of collagen synthesis by ascorbic acid and by its derivatives is discussed.

Diverse effects of ascorbic acid and palmitoyl ascorbate onHelicobacter pylorisurvival and growth

“…pylori was grown on EYE agar plates at 37 ‡C for 23 days under microaerophilic conditions and suspended in BHI broth up to a turbidity of 0.2^0.25 OD 660 nm mea- sured by M330 single beam spectrophotometer (CamSpec, UK). The suspension contained approximately 1U10 72 U10 8 CFU ml 31 and was used for determination of H. pylori survival and further inoculation of liquid growth medium. 25-ml Erlenmeyer £asks containing 10 ml BHI broth supplemented with 10% FCS, 0.1 ml selective supplement solution and L-AA as sodium ascorbate (Sigma, A-7631, USA) at a ¢nal concentration of 0.20^20 mg ml 31 (1^100 mM) were inoculated with H. pylori.…”

“…The di¡erent strains of H. pylori were pre-incubated on the EYE agar and cell density of the preparatory suspension in BHI broth was adjusted to 10 7^1 0 8 CFU ml 31 . Ethanol solutions of PA (Sigma, A-5041) or polyoxyethylene 20 stearyl ether (Brij 78) (Sigma, P-4019) over a ¢nal concentration range of 0.02^0.4 mg ml 31 were added to 2 ml of the cell suspension in plastic tubes.…”

Co-delivery of docetaxel and palmitoyl ascorbate by liposome for enhanced synergistic antitumor efficacy

“…Compared with ascorbate, PA also shows stronger inhibition on the growth of cancer cells and prolong the lifespan of tumor bearing mice22. It may alter DNA synthesis and the proliferation of tumor cells, leading to the release of membrane phospholipids and suppressing the post-transcriptional induction of ornithine decarboxylase23.…”

Nano Neurology and the Four P's of Central Nervous System Regeneration: Preserve, Permit, Promote, Plasticity

“…These obstacles are (1) scar tissue formation after tissue injury [15][16][17][18][19][20][21], (2) gaps in nervous tissue formed during phagocytosis of dying cells after injury [17,[22][23][24][25][26][27][28], (3) factors that inhibit axon growth in the mature mammalian CNS [15,17,[22][23][24][25][26][27][28][29][30][31][32][33][34], and (4) failure of many adult neurons to initiate axonal extension [17,[22][23][24][25][26]29,31,35,36]. Through reducing or overcoming the first two and possibly the first three of the barriers described above and creating a permissive environment for axonal regrowth using a synthetic biologic nanomaterial, with components that break down into beneficial building blocks and produce no adverse effects in the CNS, functional behavioral recovery becomes a reality.…”