Acute Vocal Fold Wound Healing in a Rabbit Model

Abstract

Several authors have eloquently described the characteristics of vocal fold scar, a long-term consequence of vocal fold injury. However, events in the acute stage of mucosal injury, which lead up to fibrosis, have been largely overlooked. The current study describes acute events with regard to mucosal re-formation in a rabbit model. Vocal fold injury was induced surgically. A fibrinous clot was present 1 day after injury. Massive cellular infiltration was noted on day 3, and complete epithelial coverage was achieved by day 5. Also, neo-matrix deposition was noted as early as 5 days after injury, and more mature collagen was seen by day 7. The general timetable described in the current study can contribute to the experimental foundation for the development of regenerative models of healing in the vocal folds. Most notably, the proliferation phase of wound healing appears to occur approximately 3 days after injury, indicating a critical time for intervention. Manipulation and/or alteration of naturally occurring neo-matrix deposition and organization may yield improved biophysical function of the injured vocal fold.

Investigation of Experimental Wound Closure Techniques in Voice Microsurgery

“…Three of the more commonly used models in operative studies are rabbits (Thibeault, Gray et al 2002;Thibeault, Bless et al 2003;Branski, Rosen et al 2005;Carneiro and Scapini 2009;Campagnolo, Tsuji et al 2010), dogs (Garrett, Coleman et al 2000;Fleming, McGuff et al 2001;Rousseau, Hirano et al 2003;Karajanagi, Lopez-Guerra et al 2011) and pigs (Blakeslee, Banks et al 1995;Garrett, Coleman et al 2000;Jiang, Raviv et al 2001;Alipour and Jaiswal 2008;Fonseca, Malafaia et al 2010). …”

“…Carneiro et al (Carneiro and Scapini 2009) used rabbit s to study vo cal fol d grafts by exposing their vocal folds via a neck incision and laryngofissure. Branski et al (Branski, Rosen et al 2005) studied the healing process of rabbit vocal fold after injury, using a neonatal laryngoscope to access the vocal fold. Campagnolo et al (Campagnolo, Tsuji et al 2010) studied the healing effects of injectable corticosteroids after vocal fold surgery using a custom made laryngoscope for access.…”

The Possibilities of Cell Technologies in the Treatment of Cicatricial Lesions of the Vocal Folds

“…Максимум клеточной инфильтрации наблюдается ко 2−3-м сут. На 3-й день отмечается ча-стичная эпителизация, к 5-му дню раневая поверхность полностью покрыта утолщенным эпителиальным слоем, появляется матрикс новообразованного коллагена [15,19,20]. Более плотный и неорганизованный коллаген образуется к 10-му дню.…”

Angiogenesis, fibrinogenesis and presence of synechiae after exeresis of a swine vocal fold mucosal microflap and use of topical mitomycin-C

“…Normal wound healing takes place as a sequence of well-coordinated events leading to the formation of new tissue. Alterations or therapeutic manipulation during these acute stage events can be very relevant in scar formation 4 . Conventional vocal fold treatments include the injection of autologous or heterologous materials, slicing of the superficial layer of the vocal mucosa and phonotherapy.…”