Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Copper (II) Complex against Ethanol-Induced Acute Gastric Lesions in Rats

Hajrezaie, Maryam; Golbabapour, Shahram; Hassandarvish, Pouya; Gwaram, Nura Suleiman; Hadi, A. Hamid A.; Ali, Hapipah Mohd; Majid, Nazia Abdul; Abdulla, Mahmood Ameen

doi:10.1371/journal.pone.0051537

Cited by 71 publications

(107 citation statements)

References 39 publications

(34 reference statements)

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“…Immunohistochemistry results indicated that pretreatment of rats with C. barometz extract produced upregulation of HSP70 protein, which protected the cells from oxidative stress or heat shock. Moreover, the downregulation of HSP70 protein expression is one of the types of gastric damage that was characteristic of the ulcer group, which is similar to studies reported by a huge number of coworkers 21,27,66,67. The generation of ROS by ethanol led to downregulation of HSP70 expression and upregulation of Bax proteins.…”

Section: Discussionsupporting

confidence: 85%

The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

Al-Wajeeh¹,

Hajrezaie²,

Al-Henhena³

et al. 2017

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Cibotium barometz is a pharmaceutical plant customarily used in traditional medicine in Malaysia for the treatment of different diseases, such as gastric ulcer. The gastroprotective effect of C. barometz leaves against ethanol-induced gastric hemorrhagic abrasions in Sprague Dawley rats has been evaluated in terms of medicinal properties. Seven groups of rats (normal control and ulcerated control groups, omeprazole 20 mg/kg, 62.5, 125, 250, and 500 mg/kg of C. barometz correspondingly) were used in antiulcer experiment and pretreated with 10% Tween 20. After 1 hour, the normal group was orally administered 10% Tween 20, whereas absolute alcohol was fed orally to ulcerated control, omeprazole, and experimental groups. Gastric’s homogenate were assessed for endogenous enzymes activities. Stomachs were examined macroscopically and histologically. Grossly, the data demonstrated a significant decrease in the ulcer area of rats pretreated with plant extract in a dose-dependent manner with respect to the ulcerated group. Homogenates of the gastric tissue exhibited significantly increased endogenous enzymes activities in rats pretreated with C. barometz extract associated with the ulcerated control group. Histology of rats pretreated with C. barometz extract group using hematoxylin and eosin staining exhibited a moderate-to-mild disruption of the surface epithelium with reduction in submucosal edema and leucocyte infiltration in a dose-dependent manner. In addition, it showed heat shock protein70 protein up-expression and BCL2-associated X protein downexpression. These outcomes might be attributed to the gastroprotective and antioxidative effects of the plant.

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Section: Discussionsupporting

confidence: 85%

The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

Al-Wajeeh¹,

Hajrezaie²,

Al-Henhena³

et al. 2017

DDDT

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“…For histological assessments, slides of the stomach sections were prepared at a 5 μm thickness and stained with staining of hematoxylin and eosin (H and E) [52]. …”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

et al. 2017

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Background Cibotium barometz is a medical herb used traditionally in the Malaysian peninsula for several ailments, including gastric ulcer. The aim of this study was assessment the anti-ulcer effects of C. barometz hair on ethanol-induced stomach hemorrhagic abrasions in animals. Seven groups of Sprague Dawley (SD) rats were administered 10% Tween 20 in the normal control and ulcer control groups, and omeprazole 20 mg/kg and 62.5, 125, 250, and 500 mg/kg of C. barometz hair extract in the experimental groups. After 60 min, the normal control group of rats was orally administered 10% Tween 20, while absolute ethanol was orally administered to the groups of ulcer control, omeprazole and experimental groups. Stomachs of the rats were examined macroscopically and histologically. Homogenates of stomachs were used to evaluate endogenous antioxidant enzyme activities.ResultsRats pre-fed with plant extract presented a significant decrease in the sore area, increased pH of gastric contents and preserved stomach wall mucus compared to the ulcer group. Histologically, rats pre-fed with C. barometz hair extract showed mild to moderate disruptions of the surface epithelium while animals pre-fed with absolute ethanol showed severe disruptions of the stomach epithelium with edema and leucocyte penetration of the submucosal layer. A Periodic acid Schiff (PAS) staining revealed that each rat pre-treated with the plant extract displayed an intense uptake of stomach epithelial glycoprotein magenta color compared to the ulcer control group. Immunohistochemical analysis revealed that rats pre-fed with the plant extract showed an up-regulation of the heat shock protein 70 (HSP70) and down-regulation of Bax proteins compared to ulcer control rats. Homogenates of the stomach tissue demonstrated significant increases in the endogenous antioxidant enzymatic activity and decreased lipid peroxidation (MDA) in rats pre-treated with C. barometz hair extract compared with the ulcer control rats. In acute toxicity, the liver and kidney revealed no hepatotoxic or nephrotoxic effects histologically.ConclusionsThe gastric cytoprotective action of C. barometz hair extract might be attributed to antioxidants, an increase in gastric pH, stomach mucus preservation, increased endogenous antioxidant enzymes, decreased lipid peroxidation, up-regulation of HSP70 and down-regulation of Bax proteins.

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“…This result negates the enhancing effect of YMJ on mucus production. Increased mucus secretion is among the important mechanisms of gastric mucosal defense against necrotizing agents [29, 30]. In addition, when associated with bicarbonate production, it may play a significant role in the prevention of ulcer formation and the protection of newly formed cells from acid and peptic injury [17].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of gastroprotective and ulcer healing activities of yellow mombin juice from Spondias mombin L.

et al. 2018

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Spondias mombin L. (yellow mombin) is a tree with a nutritional fruit that is commonly consumed in the North and Northeast of Brazil, as the juice of its pulp is rich in antioxidant compounds. This study aimed to investigate the gastroprotective and ulcer healing activities of yellow mombin juice (YMJ) in Wistar rats, and to elucidate the possible involved mechanisms. Phytochemical characterization of the lyophilized fruit juice was performed by high-performance liquid chromatography (HPLC). The gastroprotective activity of YMJ was investigated in ethanol (25, 50, and 100% YMJ) and indomethacin (100% YMJ) models of acute gastric ulcer in rats. Then, the effect of YMJ on mucus production and gastric secretions, and the involvement of non-protein sulfhydryl groups and prostaglandins in the gastroprotective process were examined. Moreover, the ulcer healing effect of YMJ was investigated in a model of acetic acid-induced chronic ulcer through histological and immunohistochemical analyses. HPLC results identified the presence of epicatechin (7.1 ± 1.6 μg/mL) and quercetin (17.3 ± 2.5 μg/mL) in YMJ. Ethanol-induced gastric lesions were inhibited by YMJ (25, 50, and 100%) by 42.42, 45.09, and 98.21% respectively, and indomethacin-induced lesions were inhibited by YMJ (100%) by 58.96%, compared to control group. Moreover, YMJ reduced gastric content and total acidy by 57.35 and 71.97%, respectively, compared to the control group. Treatment with YMJ also promoted healing of chronic ulcer, regeneration of the gastric mucosa, and restoration of mucus levels in glandular cells, as confirmed by histological analysis. It also increased cellular proliferation, as demonstrated by high reactivity to Ki-67 and bromodeoxyuridine. In conclusion, YMJ was found to possess gastroprotective and ulcer healing activities that are correlated to its antisecretory action. These results support the commercial exploration of YMJ as a functional food.

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Acute Toxicity and Gastroprotection Studies of a New Schiff Base Derived Copper (II) Complex against Ethanol-Induced Acute Gastric Lesions in Rats

Cited by 71 publications

References 39 publications

The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

Evaluation of gastroprotective and ulcer healing activities of yellow mombin juice from Spondias mombin L.

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