Acute phase response to recombinant interleukin‐6 and macrophage‐ and fibroblast‐derived crude cytokine preparations in primary cultures of mouse hepatocytes

Abstract

A number of acute phase proteins were determined by electroimmunoassay in media from CBA mouse hepatocytes cultured for 2 days with human recombinant IFN beta 2/IL-6, as well as with conditioned media from LPS-stimulated rat macrophages, and of murine L fibroblasts. It was found that human recombinant IL-6 caused three-fold increase in secretion of fibrinogen, while haptoglobin, complement C3 and transferrin were increased respectively, to 168 per cent, 151 per cent, and 145 per cent of the control. DEX(10(-7) M) in DMEM supplemented with 5 per cent FCS, enhanced the IL-6 effect on the three positive acute phase proteins. IL-6 elevated haptoglobin mRNA in mouse hepatocytes to a degree comparable with the concentration of the protein in the culture medium. The effect of conditioned media from murine fibroblasts and peritoneal rat macrophages was generally similar to that of recombinant IL-6. However, both natural preparations of the cytokines caused decrease in albumin and alpha-1-proteinase inhibitor secretion.

β‐Adrenergic stimulation of interleukin‐1α and interleukin‐6 expression in mouse brown adipocytes

“…We show, that stimulation of brown adipocytes by physiological concentration of NE results in activated transcription and secretion of IL‐6 into the cultivation medium up to 10 ng/ml per 24 h. Similar IL‐6 concentration in serum were sufficient to activate most of the IL‐6 biological functions including synthesis of acute phase proteins by hepatocytes [19, 20]. The physiological relevance of IL‐6 release by BAT remains open.…”

Neutrophil adherence to isolated adult cardiac myocytes. Induction by cardiac lymph collected during ischemia and reperfusion.

“…An important role for IL-6 has been previously implicated in a variety of other cellular responses such as the mediation of the acute phase reaction in hepatocytes (49,50) and the modulation of immunologic responses dependent on lymphocytes (51) functions. This report represents the first demonstration that IL-6 may induce intercellular adherence molecules on mesenchymal cells and thereby influence neutrophil functions.…”

Η Επίδραση Του Αντι TNF-α Στη Σπλαγχνική Ισχαιμία-Επαναιμάτωση Από Πνευμοπεριτόναιο

“…Κλινική μελέτη έδειξε ότι οι τιμές της IL-6 αυξάνονται σημαντικά στον ορό των ασθενών μετά από 24 ώρες από την λαπαροσκοπική επέμβαση434 . Η IL-6 έχει ρυθμιστικό ρόλο στην οξεία φάση της φλεγμονής και στην ανοσολογική απάντηση στη μόλυνση435,436 .Επιπλέον, η έκκρισή της από τα μακροφάγα, τα μονοκύτταρα και τα ενδοθηλιακά κύτταρα ενισχύεται από τον TNF-α και την IL-1437 .Στη δική μας πειραματική μελέτη οι τιμές του TNF-α στο πλάσμα ή στους ιστούς δεν προσδιορίστηκαν. Ο λόγος είναι ότι ο προσδιορισμός του TNF-α με την μέθοδο ELISA έχει διασταυρούμενη αντίδραση με την Ινφλιξιμάμπη (η Ινφλιξιμάμπη συνδέεται με το αντίσωμα του kit της ELISA) και θα λαμβάναμε ανακριβή αποτελέσματα.…”