Acute Phase Proteins in Racehorses with Inflammatory Airway Disease

Leclère, Mathilde; Lavoie‐Lamoureux, A.; Lavoie, Jean‐Pierre

doi:10.1111/jvim.12587

Cited by 36 publications

(26 citation statements)

References 22 publications

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“…The combined serum concentrations of SP‐D and haptoglobin were reported to differentiate horses with moderate asthma from healthy horses with no overlap between groups (sensitivity and specificity of 100%) . Serum haptoglobin concentration or other serum acute phase protein concentrations such as serum amyloid A or C‐reactive protein do not appear to be altered in exercise‐intolerant racehorses with mild to moderate asthma . None of these 3 studies investigated the specificity of these biomarkers by integrating other conditions responsible for exercise intolerance.…”

Section: Introductionmentioning

confidence: 99%

Investigation of blood biomarkers for the diagnosis of mild to moderate asthma in horses

Leclère

Vargas

et al. 2019

Veterinary Internal Medicne

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Background Asthma in horses is associated with nonspecific respiratory clinical signs and may be manifested only as exercise intolerance. Its diagnosis relies on bronchoalveolar lavage fluid (BALF) cytology in the presence of compatible clinical signs. The identification of blood biomarkers for this condition would facilitate diagnosis in the field, because there are regional areas where BAL is not routinely performed in clinical practice. Objective Identification of blood biomarkers for the diagnosis of asthma in horses. Animals Fourteen horses with asthma with increased neutrophil numbers in BALF (neutrophilic asthma), 9 healthy control horses, and 10 horses with other pathologic conditions (pathologic controls). Methods Physical examination, clinical score, hematology, and BALF cytology (in a subset of horses) were performed. Serum concentrations of surfactant protein D (SP‐D), haptoglobin, and secretoglobin (SCGB) were measured using commercial ELISA assays. Results Serum concentration of SP‐D > 43 ng/mL, serum concentration of haptoglobin >5730 ng/mL, and serum concentration of SCGB <19 ng/mL allowed differentiation of horses with neutrophilic asthma from horses of the control groups (healthy and pathologic) with sensitivity of 55, 95, and 75%, and specificity of 67, 28, and 60%, respectively. Specificity of 100% and sensitivity of 45% were obtained with the combination of SP‐D, haptoglobin, and SCGB at the serum concentrations indicated above. Specificity of 95% and sensitivity of 45% were obtained with the combination of SP‐D and SCGB serum concentrations. Conclusions and Clinical Importance Haptoglobin, SCGB, and SP‐D may be diagnostic aids in horses with clinical signs of lower airway disease and neutrophilic pulmonary inflammation.

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Section: Introductionmentioning

confidence: 99%

Investigation of blood biomarkers for the diagnosis of mild to moderate asthma in horses

Leclère

Vargas

et al. 2019

Veterinary Internal Medicne

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“…The combination of an organ‐specific biomarker (SP‐D) and of an acute phase protein (haptoglobin) yielded the best diagnostic results. However, the effect of other causes of reduced performances in horses, whether affecting the lungs or other organs on SP‐D is still unknown, even though our group recently reported no difference in SAA and haptoglobin concentrations in blood of racehorses with and without IAD . Also, as healthy horses were studied as controls, the effect of other pulmonary conditions on acute phase proteins could represent a potential source of bias (decreasing sensitivity and specificity of the test).…”

Section: Discussionmentioning

confidence: 93%

Serum Surfactant Protein D and Haptoglobin as Potential Biomarkers for Inflammatory Airway Disease in Horses

Bullone

Lagarde

Vargas

et al. 2015

Veterinary Internal Medicne

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BackgroundThe identification of serum biomarkers of lung inflammation would facilitate the diagnosis of inflammatory airway disease (IAD) in horses.HypothesisHorses with IAD have higher serum concentrations of markers of inflammation compared to controls.AnimalsTwelve horses with IAD and 10 control horses.MethodsThis was a prospective case–control study. Blood and BALF were collected from horses with IAD and controls. Serum concentration of surfactant protein D (SP‐D), haptoglobin, serum amyloid A (SAA) and of the soluble form of triggering receptor expressed on myeloid cells 1 (sTREM‐1) was measured using commercial ELISA tests.ResultsHorses with IAD had higher serum concentration (log‐transformed values) of SP‐D (mean ± SD: 1.773 ± 0.51), haptoglobin (6.657 ± 0.202) and SAA (0.128 ± 0.396) compared to controls (0.942 ± 0.226, 6.38 ± 0.22, −0.398 ± 0.319, respectively; P < .01 for all). Furthermore, the concentrations of SP‐D and haptoglobin combined allowed differentiating the 2 groups (IAD: 8.43 ± 0.564, controls: 7.322 ± 0.249, P < .0001) with a sensitivity and specificity of 100% when a cut‐off of 7.70 (log value) was employed.Conclusions and Clinical ImportanceSurfactant protein D and haptoglobin serum concentrations could be a diagnostic aid in IAD. Further studies are necessary to establish the specificity of our findings before they can be applied in everyday practice.

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“…Serum acute phase proteins such as serum amyloid A, C-reactive protein and haptoglobin do not appear to be altered in racehorses with IAD. 87,88…”

Section: Blood Biomarkersmentioning

confidence: 99%