Acute phase proteins in healthy and sick cats

Kann, R.; Seddon, Jennifer M.; Henning, Joerg; Meers, J.

doi:10.1016/j.rvsc.2011.11.007

Cited by 40 publications

(77 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The LOD of 0.19 g/L lies within the lower range of serum haptoglobin concentrations in healthy cats observed in previous studies using other methods. 8,13,22,23 Therefore, the LOD demonstrates sufficient analytical sensitivity for clinical use.…”

Section: Discussionmentioning

confidence: 99%

“…The commercially available spectrophotometric assay f was used as a comparative method as it has been the main measuring method for feline haptoglobin in several previous studies, 13,23,24,28 and is currently in use for routine diagnostic measurements of feline haptoglobin. The test is based on the ability of haptoglobin to bind hemoglobin.…”

Section: Spectrophotometric Assaymentioning

confidence: 99%

See 1 more Smart Citation

Validation of an enzyme-linked immunosorbent assay for measurement of feline haptoglobin

et al. 2016

View full text Add to dashboard Cite

Abstract. Haptoglobin is a positive moderate acute phase protein (APP) in cats. Measurement of haptoglobin can be used in the diagnosis, prognosis, and monitoring of systemic inflammatory disease, especially by creating profiles with major APPs. The aim of our study was to validate a sandwich enzyme-linked immunosorbent assay (ELISA) for measurement of feline haptoglobin. The validation included an assessment of precision, accuracy, detection limit, method comparison with a spectrophotometric assay, and evaluation of the overlap performance. The concentration of haptoglobin was measured in serum from 27 healthy and 23 sick cats. The coefficients of variation were 2.5-4.7% for intra-assay variability and 7.1-11.6% for interassay variability. The ratio of observed to expected dilutional parallelism of 4 serum samples was 108.1-118.4%. The ratio of observed to expected spike recovery of 4 serum samples was 90.8-94.0%. The lower detection limit was 0.19 g/L. Method comparison revealed a positive correlation (r s = 0.949, P < 0.0001) and a proportional bias between the methods of −38.9%. Agreement between the methods was not clinically acceptable. Overlap performance of the ELISA was deemed satisfactory. The sandwich ELISA measures feline haptoglobin with an analytical and overlap performance acceptable for clinical purposes. Given the observed bias, the ELISA cannot be used interchangeably with the spectrophotometric assay.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Spectrophotometric Assaymentioning

confidence: 99%

Validation of an enzyme-linked immunosorbent assay for measurement of feline haptoglobin

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In addition, AGP in cat serum or effusion samples is a recognised biomarker of FIP (6,21,25). However, these APP concentrations increase not only in inflammatory diseases, but also in several non-inflammatory disorders, such as neoplasia, diabetes mellitus and hyperthyroidism (10,22,27). In the present study, changes in APPs in cats with anaemia were investigated.…”

Section: Methodsmentioning

confidence: 91%

“…K-ASSAY, Seattle, USA) concentrations were analysed using specific commercially available test kits in an ELISA reader device. SAA (Solid phase sandwich ELISA kit Tridelta Development LTD. Ireland) and serum Hp (Tridelta Development LTD. Ireland) concentrations were determined using specific commercially available test kits previously validated for use in cats (10,14).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Serum concentrations of some acute phase proteins in cats with anaemia

Tuna¹,

Dinler²,

̃ici³

et al. 2018

Medycyna Weterynaryjna

View full text Add to dashboard Cite

SummarySerum concentrations of acute phase proteins can provide valuable diagnostic information in the detection and monitoring of disease. The available information on the acute phase response in cats with anaemia is limited. The aim of this study was to retrospectively evaluate serum concentrations of haptoglobin, serum amyloid A, α1 acid glycoprotein and their clinical importance in cats with anaemia. Thirty-four anaemic cats and ten healthy cats were enrolled this study. After individual diagnoses had been established, the cats were divided into three groups (healthy group, haemolytic group and non-haemolytic group). Serum acute phase protein concentrations were analysed using specific commercially available test kits in an ELISA reader device. Serum amyloid A and serum α1 acid glycoprotein concentrations were significantly higher in the anaemic groups compared with the healthy group. Haptoglobin concentrations were significantly higher in cats from the non-haemolytic anaemia group than they were in healthy animals and those from the haemolytic anaemia group. Although serum haptoglobin concentrations were lower than in the healthy group, there was no significant difference between the haemolytic anaemia group and the healthy group. The results of this study suggest that serum amyloid A and α1 acid glycoprotein could be useful in the diagnosis and determination of inflammation in cats with anaemia. Serum haptoglobin depletion may be used for diagnosis of haemolysis in cats with haemolytic anaemia. In addition, this study has contributed to the limited data available on acute phase protein concentrations in cats with anaemia.

show abstract