Acute lung injury induced by lipopolysaccharide is inhibited by wogonin in mice via reduction of Akt phosphorylation and RhoA activation

Yeh, Yen-Cheng; Yang, Ching-Ping; Lee, Shiuan-Shinn; Horng, Chi‐Ting; Chen, Hung-Yi; Cho, Ta-Hsiung; Yang, Ming-Ling; Lee, Chien‐Ying; Li, Miao-Cing; Kuan, Yu‐Hsiang

doi:10.1111/jphp.12500

Cited by 26 publications

(14 citation statements)

References 32 publications

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“…The levels of phosphorylated ERK1/2 and p38 were significantly reduced after APS pretreatment. In addition to MAPK, NF‐κB is also involved in the regulation of the expression of proinflammatory mediators (Ho et al, 2017; Yeh et al, 2016). NF‐κB p65 protein binds to IκB protein and is present in the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

“…The levels of phosphorylated ERK1/2 and p38 were significantly reduced after APS pretreatment. In addition to MAPK, NF-κB is also involved in the regulation of the expression of proinflammatory mediators(Ho et al, 2017;Yeh et al, 2016).NF-κB p65 protein binds toIκB protein and is present in the cytoplasm. When IECs are stimulated by external stimuli, NF-κB kinases (IKKs) can phosphorylate the IκB family of proteins (Diomede et al, 2017), followed by ubiquitination and degradation, further phosphorylating p65 and releasing it into the nucleus, where it promotes inflammation.…”

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confidence: 99%

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Astragalus polysaccharides alleviates LPS‐induced inflammation via the NF‐κB/MAPK signaling pathway

Dong

Xue

et al. 2020

Journal Cellular Physiology

131

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Early weaning usually causes intestinal disorders, enteritis, and diarrhea in young animals and human infants. Astragalus polysaccharides (APS) possesses anti‐inflammatory activity. To study the anti‐inflammatory mechanisms of APS and its potential effects on intestinal health, we performed an RNA sequencing (RNA‐seq) study in lipopolysaccharide (LPS)‐stimulated porcine intestinal epithelial cells (IPEC‐J2) in vitro. In addition, LPS‐stimulated BALB/c mice were used to study the effects of APS on intestinal inflammation in vivo. The results from the RNA‐seq analysis show that there were 107, 756, and 5 differentially expressed genes in the control versus LPS, LPS versus LPS+APS, and control versus LPS+APS comparison groups, respectively. The results of Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the mitogen‐activated protein kinase (MAPK) and nuclear factor‐κB (NF‐κB) signaling pathways play significant roles in the regulation of inflammatory factors and chemokine expression by APS. Further verification of the above two pathways by using western blot and immunofluorescence analysis revealed that the gene expression levels of the phosphorylated p38 MAPK, ERK1/2, and NF‐κB p65 were inhibited by APS, while the expression of IκB‐α protein was significantly increased (p < .05), indicating that APS inhibits the production of inflammatory factors and chemokines by the inhibition of activation of the MAPK and NF‐κB inflammatory pathways induced by LPS stimulation. Animal experiments further demonstrated that prefeeding APS in BALB/c mice can alleviate the expression of the jejunal inflammatory factors interleukin 6 (IL‐6), IL‐Iβ, and tumor necrosis factor‐α induced by LPS stimulation and improve jejunal villus morphology.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Astragalus polysaccharides alleviates LPS‐induced inflammation via the NF‐κB/MAPK signaling pathway

Dong

Xue

et al. 2020

Journal Cellular Physiology

131

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“…Wogonin 15 , a natural flavonoid extracted from Scutellaria baicalensis Georgi, prevented lung injury in ALI murine model by suppressing production of inflammatory cytokines TNF-α, IL1-β, IL-6, iNOS, COX-2 and MIP-2. In terms of mechanism, wogonin blocked Akt and RhoA activation, reduced p38 MAPK and JNK phosphorylation, as well as suppressed the peroxisome proliferator-activated receptor gamma (PPARγ)-involved NF-κB signaling pathway [ [138] , [139] , [140] ]. Of note, 10 mg/kg wogonin exhibited inhibitory effects on lung edema as well as expression of iNOS and COX-2 comparable to that of 1 mg/kg DEX in an LPS-induced ALI murine model [ 139 ].…”

Section: Natural Compounds That Exert Anti-ali Effectsmentioning

confidence: 99%

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Zhou

et al. 2021

Pharmacological Research

224

121

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“…Furthermore, LPS is demonstrated to be involved in excessive inflammatory cascade under various pathological conditions. It is well established that LPSinduced ALI is the most famous and popular animal models in literature, which has been performed extensively [17][18][19]. Hence, rats underwent LPS administration to establish neonatal ALI model in the present study.…”

Section: Discussionmentioning

confidence: 96%

Narciclasine attenuates LPS-induced acute lung injury in neonatal rats through suppressing inflammation and oxidative stress

Duan

Yin

et al. 2020

Bioengineered

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Acute lung injury (ALI) is a life-threatening disorder related to serious pulmonary inflammation. Narciclasine exhibits strong anti-inflammation activity and attenuates the reactive oxygen species (ROS) production. The present study aims to investigate the underlying mechanism related to the effect of narciclasine on the pathogenesis of neonatal acute lung injury (ALI). Narciclasine attenuated LPS-induced pathological injury and pulmonary edema. In addition, narciclasine suppressed the secretion of inflammatory cytokines, including necrosis factor-α (TNF-α), Interleukin (IL-6), IL-1β, monocyte chemotactic protein-1 (MCP-1) in serum, and inhibited the expressions of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in lung tissues of neonatal ALI rats. Furthermore, narciclasine alleviated oxidative stress and apoptosis in lung tissues. Importantly, narciclasine exerted an inhibition effect on NF-κB nuclear translocation and activation of Toll-like Receptor 4 (TLR4)/Nuclear factor (NF)-κB/ Cyclooxygenase 2 (Cox2) signaling pathway. Taken together, narciclasine protected against lung injury via inhibition effect on excessive inflammation, oxidative stress and apoptosis, hence, narciclasine may be considered as an effective and novel agent for clinical therapeutic strategy of ALI Treatment.

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Acute lung injury induced by lipopolysaccharide is inhibited by wogonin in mice via reduction of Akt phosphorylation and RhoA activation

Abstract: These results suggested that the effects of wogonin in LPS-induced ALI were induced by inhibition of Akt phosphorylation and RhoA activation.

Cited by 26 publications

References 32 publications

Astragalus polysaccharides alleviates LPS‐induced inflammation via the NF‐κB/MAPK signaling pathway

Astragalus polysaccharides alleviates LPS‐induced inflammation via the NF‐κB/MAPK signaling pathway

Natural product derived phytochemicals in managing acute lung injury by multiple mechanisms

Narciclasine attenuates LPS-induced acute lung injury in neonatal rats through suppressing inflammation and oxidative stress

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