Acute hepatic response to diet modification and exercise-induced endotoxemia during a laboratory-based duathlon

Moncada-Jiménez, José; Plaisance, Eric P.; Ramírez, Fernando; Taylor, J. Kyle; Ratcliff, Lance; Mestek, Michael L.; Grandjean, Peter W.; Vargas, Luis Fernando Aragón

doi:10.5604/20831862.913077

Cited by 4 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Positively, no GI barrier integrity or MT biomarker displayed statistical heteroskedasticity in this EHST model, suggestive that absolute reliability was not dependent upon the magnitude of biomarker response. Next, biomarker analysis was limited to a single time‐point after the EHST (at termination), though this can be justified in that peak responses are consistently shown to occur at this instance in comparable exertional heat stress interventions (e.g., I‐FABP , Snipe et al, ; CLDN‐3 , Yeh et al, ; LBP , Moncada‐Jimenez et al, ; Bact./16S , March et al, ). Third, there was statistically significant systematic bias for peak T core and T body responses, which were lower (0.17°C and 0.18°C) following implementation of trial two.…”

Section: Limitationsmentioning

confidence: 99%

Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress

Ogden

Fallowfield²,

Child

et al. 2020

Physiol Rep

View full text Add to dashboard Cite

Purpose Exertional heat stress adversely distrupts (GI) barrier integrity and, through subsequent microbial translocation (MT), negativly impacts health. Despite widespread application, the temporal reliability of popular GI barrier integity and MT biomarkers is poorly characterised. Method Fourteen males completed two 80‐min exertional heat stress tests (EHST) separated by 7–14 days. Venous blood was drawn pre, immediately‐ and 1‐hr post both EHSTs. GI barrier integrity was assessed using the serum Dual‐Sugar Absorption Test (DSAT), Intestinal Fatty‐Acid‐Binding Protein (I‐FABP) and Claudin‐3 (CLDN‐3). MT was assessed using plasma Lipopolysaccharide Binding Protein (LBP), total 16S bacterial DNA and Bacteroides DNA. Results No GI barrier integrity or MT biomarker, except absolute Bacteroides DNA, displayed systematic trial order bias (p ≥ .05). I‐FABP (trial 1 = Δ 0.834 ± 0.445 ng ml−1; trial 2 = Δ 0.776 ± 0.489 ng ml−1) and CLDN‐3 (trial 1 = Δ 0.317 ± 0.586 ng ml−1; trial 2 = Δ 0.371 ± 0.508 ng ml−1) were increased post‐EHST (p ≤ .01). All MT biomarkers were unchanged post‐EHST. Coefficient of variation and typical error of measurement post‐EHST were: 11.5% and 0.004 (ratio) for the DSAT 90‐min postprobe ingestion; 12.2% and 0.004 (ratio) at 150‐min postprobe ingestion; 12.1% and 0.376 ng ml−1 for I‐FABP; 4.9% and 0.342 ng ml−1 for CLDN‐3; 9.2% and 0.420 µg ml−1 for LBP; 9.5% and 0.15 pg µl−1 for total 16S DNA; and 54.7% and 0.032 for Bacteroides/total 16S DNA ratio. Conclusion Each GI barrier integrity and MT translocation biomarker, except Bacteroides/total 16S ratio, had acceptable reliability at rest and postexertional heat stress.

show abstract

Section: Limitationsmentioning

confidence: 99%

Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress

Ogden

Fallowfield²,

Child

et al. 2020

Physiol Rep

View full text Add to dashboard Cite

show abstract

“…Therefore, increased GI permeability, and the subsequent rise in circulating LPS, can exacerbate thermal strain by initiating an inflammatory cascade, perfusion abnormalities, and organ dysfunction [ 4 ]. These alterations in GI permeability are often expressed as increased concentrations of intestinal fatty acid binding protein (I-FABP) [ 13 , 19 , 20 , 21 ], lactulose to rhamnose ratio (L:R) [ 13 , 20 , 21 , 22 , 23 ], and lipopolysaccharide-binding protein (LPS-LPB) [ 24 ]. Following an Ironman distance triathlon, 68% of athletes exhibited an at least 150% increase in LPS [ 17 ].…”

Section: Lipopolysaccharide and Endotoxemia During Exercisementioning

confidence: 99%

“…Furthermore, following a short-course duathlon, a recent study reported higher dehydration levels and a mild increase in markers of hepatic damage in those with a low-carbohydrate diet compared to a high-carbohydrate diet. However, acute exercise-induced mild endotoxemia (increased LPS) was present in both dietary conditions and did not alter physical performance or IL-6 response [ 24 ].…”

Section: Nutrition and Supplementation Strategies For Exercise In mentioning

confidence: 99%

Nutrition and Supplementation Considerations to Limit Endotoxemia When Exercising in the Heat

Guy

Vincent

2018

Sports

View full text Add to dashboard Cite

Exercise-induced heat production is further elevated by exercise performed in hot conditions and this can subsequently impact inflammation, and gastrointestinal (GI) health. Implementing nutrition and supplementation strategies under these conditions may support the hyperthermic response, the systemic inflammatory response, GI permeability and integrity, and exercise performance. Therefore, the aim of this brief review is to explore athletes’ inflammatory response of two key biomarkers, lipopolysaccharide (LPS), and interleukin-6 (IL-6), and provide nutrition and supplementation recommendations when exercising in hot conditions. There is emerging evidence that probiotics, glutamine, and vitamin C can preserve GI integrity, which may improve performance during exercise in the heat. Glucose rich food when consumed with water, before and during exercise in the heat, also appear to limit endotoxemia, preserve GI integrity, and reduce the incidence of GI disturbances compared with water alone. The use of non-steroidal anti-inflammatory drugs (NSAIDs) may compromise GI integrity and this may result in greater leakage of endotoxins during long duration exercise in the heat. Further work is required to elucidate the impact of nutrition and supplementation strategies, in particular the use of NSAIDs, when exercising in the heat.

show abstract

“…One paper reported metabolism and duathlon performance resulting from two different short-term diets (Moncada-Jiménez et al, 2009a), another one compared the acute hepatic response to initial endogenous hepatic energy levels (which differed according to the two short-term diets) and exercise-induced endotoxemia (including measurements of liver damage markers, body core temperature, and IL-6 responses) (Moncada-Jiménez et al, 2010), and the third reported the association between the initial metabolic state with its accompanying exercise-induced endotoxaemia, and the appearance of gastrointestinal symptoms during the high intensity endurance exercise test (Moncada-Jiménez et al, 2009b). Only one of these manuscripts made reference to another, but the analyses may be considered exhaustive and different enough to warrant separate papers; the experiment had been designed with those different objectives from the very beginning, considering the costbenefit of running three separate experiments.…”

Section: Avoidance Of Redundant Publicationsmentioning

confidence: 99%

THE ETHICS OF SCIENTIFIC RESEARCH (WITH PARTICULAR EMPHASIS ON EXERCISE AND MOVEMENT SCIENCE) (English translated version)

Vargas

2015

PensarMov.

View full text Add to dashboard Cite

This paper reviews how we arrived at the current state of affairs in the ethical practice of scientific research, discussing some issues that are particularly pertinent to the exercise scientist. The paper focuses on two major areas of ethics in science. The ethical principles for biomedical research involving human subjects are presented and discussed using the three basic principles from the Belmont Report (autonomy, beneficence, and justice) as a guide. The ethical presentation and publication of data are discussed as an update or expanded comment on the ten topics covered by Roy Shephard in his Ethics in Exercise Science Research paper from 2002. The manuscript closes with a reflection on personal responsibility and its importance in every scientific endeavor: placing all responsibility for action on those scientists or physicians doing the experiments was not sufficient to prevent all types of human research abuses in the first half of the twentieth century. However, intricate and cumbersome external review and approval procedures generate the perception that the system should be more than enough to ensure good practices, a perception that may dangerously prevent the scientists from assuming their individual responsibility.

show abstract

Acute hepatic response to diet modification and exercise-induced endotoxemia during a laboratory-based duathlon

Cited by 4 publications

References 52 publications

Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress

Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress

Nutrition and Supplementation Considerations to Limit Endotoxemia When Exercising in the Heat

THE ETHICS OF SCIENTIFIC RESEARCH (WITH PARTICULAR EMPHASIS ON EXERCISE AND MOVEMENT SCIENCE) (English translated version)

Contact Info

Product

Resources

About