Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure

Çomaklı, Selim; Sevim, Çiğdem; Kontadakis, Georgios A.; Doğan, Elif; Taghizadehghalehjoughi, Ali̇; Özkaraca, Mustafa; Aschner, Michael; Νikolouzakis, Τaxiarchis Κonstantinos; Tsatsakis, Aristidis

doi:10.1016/j.toxrep.2019.01.004

Cited by 14 publications

(8 citation statements)

References 48 publications

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“…In contrast, glufosinate exposure to eyes increased intralocular pressure in mice suggesting that the herbicide could damage the optic nerves when improperly handled. 39 The sub-chronic feeding studies of glufosinate were carried out over 3 months at various concentrations using rats (up to 4000 mg kg −1 diet), mice (up to 1280 mg kg −1 diet), and dogs (up to 256 mg kg −1 diet). 40 The 'no observed adverse effect levels' were 2-4 mg kg −1 d −1 for dogs, 4.1 for rats and 17 for mice.…”

Section: Glufosinate Toxicologymentioning

confidence: 99%

“…In the same study, glufosinate was non‐sensitizing to dermal or eye exposure, but was moderately irritating to the eyes due to some of the adjuvants in the formulation. In contrast, glufosinate exposure to eyes increased intralocular pressure in mice suggesting that the herbicide could damage the optic nerves when improperly handled 39 . The sub‐chronic feeding studies of glufosinate were carried out over 3 months at various concentrations using rats (up to 4000 mg kg −1 diet), mice (up to 1280 mg kg −1 diet), and dogs (up to 256 mg kg −1 diet) 40 .…”

Section: Glufosinate Toxicologymentioning

confidence: 99%

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Glufosinate‐ammonium: a review of the current state of knowledge

Takano

Dayan

2020

Pest Management Science

133

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Glufosinate is a key herbicide to manage glyphosate-resistant weeds mainly because it is a broad-spectrum herbicide, and transgenic glufosinate-resistant crops are available. Although glufosinate use has increased exponentially over the past decade, the treated area with this herbicide is far less than that with glyphosate. This is because glufosinate often provides inconsistent performance in the field, which is attributed to several factors including environmental conditions, application technology, and weed species. Glufosinate is also highly hydrophilic and does not translocate well in plants, generally providing poor control of grasses and perennial species. In the soil, glufosinate is rapidly degraded by microorganisms, leaving no residual activity. While there have been concerns regarding glufosinate toxicology, its proper use can be considered safe. Glufosinate is a fast-acting herbicide that was first discovered as a natural product, and is the only herbicide presently targeting glutamine synthetase. The mode of action of glufosinate has been controversial, and the causes for the rapid phytotoxicity have often been attributed to ammonia accumulation. Recent studies indicate that the contact activity of glufosinate results from the accumulation of reactive oxygen species and subsequent lipid peroxidation. Glufosinate disrupts both photorespiration and the light reactions of photosynthesis, leading to photoreduction of molecular oxygen, which generates reactive oxygen species. The new understanding of the mode of action provided new ideas to improve the herbicidal activity of glufosinate. Finally, a very few weed species have evolved glufosinate resistance in the field, and the resistance mechanisms are generally not well understood requiring further investigation.

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Section: Glufosinate Toxicologymentioning

confidence: 99%

Section: Glufosinate Toxicologymentioning

confidence: 99%

Glufosinate‐ammonium: a review of the current state of knowledge

Takano

Dayan

2020

Pest Management Science

133

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“…Glutamate is known as an excitatory neurotransmitter and plays a crucial role in the plasticity of the central nervous system (CNS) (1,2). When the glutamate concentration is abnormally high, it functions as a neurotoxin and leads to cell death (3). Excessive glutamate levels damage cellulr components, including the mitochondria, and promote the generation of reactive oxygen species (ROS) and induce cell death (4).…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells

et al. 2019

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The aim of this study was to investigate the antioxidant and anti-apoptotic activities, as well as the underlying mechanisms of action, of Scrophularia buergeriana ( S. buergeriana ) extract (SBE) in glutamate-induced SH-SY5Y cell death. The roots of S. buergeriana were extracted with 70% ethanol, and standardized SBE was used in this study. To induce cytotoxicity, the SH-SY5Y cells were exposed to glutamate for 3 h, or pre-treated with SBE for 1 h, and subsequently incubated with glutamate for 3 h. The neuro-protective effects were assessed by measuring cell viability and the total glutathione contents using commercial kits. The antioxidant and anti-apoptotic mechanisms of action of SBE were evaluated by western blot analysis. The results confirmed that glutamate-induced toxicity was caused by reactive oxygen species (ROS) production, leading to oxidative stress and DNA damage, thus leading to cell death. However, treatment of the SH-SY5Y cells with SBE significantly increased the viability of the cells exposed to glutamate by upregulating the levels of antioxidant proteins, such as superoxide dismutase (SOD)1, SOD2 and glutathione peroxidase-1 (GPx-1), and directly enhancing the total glutathione contents. Furthermore, SBE attenuated DNA impairment and decreased B-cell lymphoma-2 (Bcl-2)-associated X protein (Bax), cleaved caspase-3 and cleaved poly(adenosine diphosphate (ADP)-ribose) polymerase (PARP) activation. In addition, SBE upregulated Bcl-2 expression via p38 mitogen-activated protein kinases (MAPKs). On the whole, the findings of this study demonstrated that SBE exerts neuroprotective effects against glutamate-induced cell toxicity through its antioxidant and anti-apoptotic activities.

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“…The use of pesticides in agriculture, industry, and domestic applications results in increased exposure to these chemicals (Sevim et al, 2019). As a result of acute exposure, it has been observed that human health is highly affected.…”

Section: Introductionmentioning

confidence: 99%

“…As a result of acute exposure, it has been observed that human health is highly affected. Glufosinate is one of the most widely used herbicide-based pesticides worldwide (Comakli et al, 2019). Glufosinate, which is one of the main pollutants of rivers and various water resources, has a toxic effect not only on organisms, but also on food, feed and ecosystems (Singh et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study

Yilmaz

Taghizadehghalehjoughi

Hacımüftüoğlu

et al. 2021

Journal of Anatolian Environmental and Animal Sciences

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Aloe vera is one of the medicinal plants whose importance has been better understood recently with its antibacterial, antimicrobial, antioxidant and anticancer properties. Although it is known that the aloe vera family has protective effects on neurons, the neuroprotective effect of the aloe barbadensis miller plant has not yet been fully explained. Glufosinate is structurally similar to glutamate and is an herbicide that blocks glutamine synthesis. Glutamate has been shown to induce cyclooxygenase-2 (COX-2), which causes toxicity. In the present study, neuron culture was exposed to Aloe vera barbadensis Miller plant extracts plant (25, 50, 100, 200, 400, 800, and 1600 µgr/ml doses) for 24 hours to protect against glufosinate (200 mM) and glutamate (10 -5 mM) toxicity. After 24 hours, MTT, TAC, and TOS analyzes were performed and the results were revealed. In our study, it was seen that the aqueous extract of aloe barbadensis miller plant, glufosinate, and glutamate, could preserve the vitality of neurons (89% protection in AVB 400 µgr/ml group). At the same time, it was seen that while increasing the antioxidant level in neurons, it decreased the oxidant level. The group that increases the antioxidant value best is AVB 400 µgr/ml (the group that increases it 1.4 times). When the findings were evaluated, it was concluded that aloe vera and its components may have a neuroprotective effect.

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Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure

Abstract: Graphical abstract

Cited by 14 publications

References 48 publications

Glufosinate‐ammonium: a review of the current state of knowledge

Glufosinate‐ammonium: a review of the current state of knowledge

Neuroprotective effects of Scrophularia buergeriana extract against glutamate-induced toxicity in SH-SY5Y cells

Investigation of Aloe Vera Barbadensis Miller Leaf Extract Effects On Glutamate and Glyphosate Induced Toxicity: In Vitro Study

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