Acute exposure of Siberian sturgeon (Acipenser baeri, Brandt) yearlings to nitrite: median-lethal concentration (LC50) determination, haematological changes and nitrite accumulation in selected tissues

Huertas, Mar; Gisbert, Enric; Rodríguez, Adriana; Cardona, Luís; Williot, Patrick; Castelló‐Orvay, F.

doi:10.1016/s0166-445x(01)00207-7

Cited by 56 publications

(43 citation statements)

References 31 publications

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“…The higher concentration of chlorides therefore protects fish against the toxic impact of nitrite via the competition between chloride and nitrite ions transporting across the gill membrane (Jensen, 2003). The positive effect of chloride on tolerance to nitrites has been demonstrated in other studies in fish (Hilmy et al, 1987;Atwood et al, 2001;Huertas et al, 2002;Fuller et al, 2003;Taveres and Boyd, 2003) and crayfish (Beitinger and Huey, 1981;Jeberg and Jensen, 1994;Kozák et al, 2005). This study was concerned with 1) a preliminary testing of the reactivity of crayfish to the common chemical, nitrite, to gain information about sensitiveness to an increasing level of chemical stimuli up to the sublethal-lethal concentration and 2) to evaluate if chlorides could help crayfish to withstand stress in high nitrite concentration conditions.…”

Section: Introductionmentioning

confidence: 77%

Stress reaction in crayfish: chlorides help to withstand stress in high nitrite concentration conditions – preliminary study

Kozák

Policar

Fedotov³

et al. 2011

Knowl. Managt. Aquatic Ecosyst.

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Key-words:Astacus leptodactylus, cardiac activity, heart rate, salinity, stress reaction A non-invasive method of recording cardiac activity (heart rate -HR) and stress reaction (stress index -SI) was used to understand the immediate and ongoing stress reaction of crayfish to the chemical stimuli. This method detects changes in the shape and amplitude parameters of the response to the stress factors, which characterized the crayfish functional state. Experimental animals (Astacus leptodactylus) were divided to the two groups with (400 mg·L −1 Cl − ) and without added chlorides and then exposed to a stepwise increased level of nitrite to the final (sublethallethal) concentration of 60 mg·L −1 N-NO − 2 within 24 hours. The course of crayfish reaction was evident and provided information about their reaction to the sublethal-lethal concentration over time. As expected, a less prominent stress reaction was detected in the group with chlorides. The non-invasive method successfully evaluated the sensing of chemical stimuli in water through HR and SI changes. RÉSUMÉRéaction au stress chez l'écrevisse : les chlorures aident à résister au stress dans des conditions de fortes concentrations en nitrite -étude préliminaire Mots-clés :Astacus leptodactylus, activité cardiaque, rythme cardiaque, salinité, réaction au stress Une méthode non-invasive d'enregistrement de l'activité cardiaque (rythme cardiaque -HR) et de réaction au stress (indice de stress -SI) a été utilisée pour comprendre la réaction au stress immédiate et ultérieure de l'écrevisse à des stimuli chimiques. Cette méthode détecte les changements dans la forme et l'amplitude des paramètres de la réponse aux facteurs de stress, qui caractérisent l'état fonctionnel de l'écrevisse. Les animaux de l'expérience (Astacus leptodactylus) ont été divisés en deux groupes avec (400 mg Cl − ·L −1 ) et sans chlorures ajoutés puis exposés à des niveaux croissants par paliers de nitrite jusqu'à la concentration finale (sub-létale-létale) de 60 mg·L −1 N-NO − 2 pendant 24 heures. La réaction de l'écrevisse est évidente et fournit des informations sur les réactions au cours du temps à une concentration sub-létale. Comme escompté, une réaction au stress moins marquée est détectée dans le groupe avec des chlorures. La méthode noninvasive a déterminé correctement l'impact du stimulus chimique de l'eau par les changements de HR et SI.

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Section: Introductionmentioning

confidence: 77%

Stress reaction in crayfish: chlorides help to withstand stress in high nitrite concentration conditions – preliminary study

Kozák

Policar

Fedotov³

et al. 2011

Knowl. Managt. Aquatic Ecosyst.

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“…These contaminants which are obtained from the aquatic environment and food cover a wide spectrum of gases, chemicals and solids. Most toxicological studies have been limited to the effects of lethal or acute doses of these pollutants (Martinez & Souza 2002, Huertas et al 2002, Das et al 2004a, whereas, subtle physiological disorders result with exposure to sub-lethal/low doses of toxicants (Das et al 2004b,Varo et al 2007, Kori-Siakpere et al 2011. Various workers have reported histopathological changes in fish organs (e.g.…”

Section: Introductionmentioning

confidence: 99%

Histopathological effect of sub-lethal concentration of aluminum phosphide (phostoxin) on Clarias gariepinus juveniles

2016

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O.A. 2016. Histopathological effect of sublethal concentration of aluminum phosphide (phostoxin) on Clarias gariepinus juveniles. Pesquisa Veterinária Brasileira 36(7):574-580. Department of Anatomy, Lagos State University College of Medicine, Ikeja, Lagos, Nigeria. E-mail: mbaaka2gm@gmail.comThe study evaluated the effect of sub-lethal concentration of phostoxin on Clarias gariepinus juveniles. C. gariepinus juveniles belonging to the same cohort (40.1±1.2g; 18.1±1.1cm) from a commercial fish farm were randomly placed ten in each of 15 plastic tanks containing 15 liters of water. They were exposed for 96 hrs to three sub-lethal concentrations (treatments) of phostoxin (0.125, 0.250, 0.5mg L -1 ) and a phostoxin free control. At the end of 96 hrs exposure, they were dissected and the tissues need for histopathology removed and fixed in Bouin's fluid. The gill filament exhibited fusion at the secondary lamella that was progressive with concentration. At the highest concentration of exposure, the secondary lamellae showed marked pyknotic and necrotic changes characterized by epithelia detachment. The hepatic tissue showed mild inflammatory changes at lower concentrations while at the highest concentration of exposure there was marked inflammation with observed hydropic degeneration. In the kidney, an inflammatory change was only observed in the interstices at the highest dose of exposure with the convoluted tubules showing partial shrinkage. Phostoxin showed to have significantly caused alterations in cyto-architecture of the gills and to a considerable extent liver and kidney of C. gariepinus.

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“…The competition between chloride and nitrite ions transport across the gill membrane explains because the higher concentration of Cl -protects fish against toxic impact of nitrite (JENSEN, 2003). The positive effect of chloride for fish tolerance to nitrites has been demonstrated in other studies in fish (SVOBODOVÁ et al, 1987;HILMY, 1987;ATWOOD et al, 2001;HUERTAS et al, 2002;TAVARES and BOYD, 2003;FULLER et al, 2003) and for crayfish (JEBERG and JENSEN, 1994;BEITINGER and HUEY, 1981). CRAWFORD and ALLEN (1977) showed that toxicity of nitrite depended on water salinity.…”

Section: Introductionmentioning

confidence: 75%

Of Sodium Nitrite for Spiny-Cheek Crayfish (Orconectes Limosus Raf.) the Effect of Chloride Content in Water on the Toxicity

Kozák

Máchová

Policar

2005

Bull. Fr. Pêche Piscic.

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Toxicity of sodium nitrite for the spiny-cheek crayfish (Orconectes limosus) was assessed in relation to the concentration of chlorides diluted in water. Results of the tests confirmed a favourable effect of chloride content in water on the tolerance of spiny-cheek crayfish to nitrites. The 96hLC 50 value of 4.8 mg l -1 , 17.7 mg l -1 , 34.7 mg l -1 , 50.5 mg l -1 , 74.4 mg l -1 , and 96.6 mg l -1 N-NO 2 -found out in individual tests corresponded, respectively to a chloride content of 11, 40, 100, 200, 300 and 400 mg l -1 Cl -in diluting water. Linear relationship of the 96hLC 50 values on chloride concentration (y = 0.23 ×+ 7) was proven statistically (R = 0.995).

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Acute exposure of Siberian sturgeon (Acipenser baeri, Brandt) yearlings to nitrite: median-lethal concentration (LC50) determination, haematological changes and nitrite accumulation in selected tissues

Cited by 56 publications

References 31 publications

Stress reaction in crayfish: chlorides help to withstand stress in high nitrite concentration conditions – preliminary study

Stress reaction in crayfish: chlorides help to withstand stress in high nitrite concentration conditions – preliminary study

Histopathological effect of sub-lethal concentration of aluminum phosphide (phostoxin) on Clarias gariepinus juveniles

Of Sodium Nitrite for Spiny-Cheek Crayfish (Orconectes Limosus Raf.) the Effect of Chloride Content in Water on the Toxicity

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