Abstract:In the present study, we investigated the role of angiotensin type I (AT1) receptor in reactive oxygen species (ROS) generation and mitogen-activated protein kinases (MAPK) activation induced by acute ethanol intake in resistance arteries. We also evaluated the effect of ethanol on platelet-derived growth factor receptors (PDGF-R) phosphorylation and the role of this receptor on ROS generation by ethanol. Ethanol (1 g/kg; p.o. gavage) effects were assessed within 30 min in male Wistar rats. Acute ethanol intak… Show more
“…CAT activity was determinate as previously described [21]. One CAT unit (U) was defined as the amount of enzyme required to decompose 1 µmol of H 2 O 2 /min.…”
Section: Determination Of Cat Activity In Plasma and CCmentioning
confidence: 99%
“…The concentration of GSH in the CC and plasma were evaluated as previously described [21]. Results are expressed as µg GSH/mg protein or µg GSH/ml of plasma.…”
Section: Determination Of Reduced Glutathione (Gsh) In Plasma and CCmentioning
“…CAT activity was determinate as previously described [21]. One CAT unit (U) was defined as the amount of enzyme required to decompose 1 µmol of H 2 O 2 /min.…”
Section: Determination Of Cat Activity In Plasma and CCmentioning
confidence: 99%
“…The concentration of GSH in the CC and plasma were evaluated as previously described [21]. Results are expressed as µg GSH/mg protein or µg GSH/ml of plasma.…”
Section: Determination Of Reduced Glutathione (Gsh) In Plasma and CCmentioning
“…The lucigenin-derived chemiluminescence assay was used to determine O 2 − levels in aortic homogenates as previously described [19][20][21].…”
Section: Detection Of Aortic O 2 − By Lucigenin Enhanced Chemiluminesmentioning
confidence: 99%
“…The aorta was homogenized in phosphate buffer with a glass-to-glass homogenizer as previously described [21]. Reaction buffer was added to quartz cuvettes containing 20 μl of the supernatant.…”
Section: Determination Of Superoxide Dismutase (Sod) and Catalase (Camentioning
confidence: 99%
“…Aortic GSH was evaluated through its interaction with 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) as previously described [21]. Results are expressed as μg GSH/mg protein.…”
Section: Determination Of Reduced Glutathione (Gsh) In the Rat Aortamentioning
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