Acute blockade of nitric oxide synthesis induces disorganization and amplifies lesion-induced plasticity in the rat retinotectal projection

Campello-Costa, Paula; Fosse, A.M.; Ribeiro, João C.; Paes‐de‐Carvalho, Roberto; Serfaty, Claudio Alberto

doi:10.1002/1097-4695(20000915)44:4<371::aid-neu1>3.0.co;2-x

Cited by 32 publications

(8 citation statements)

References 54 publications

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“…Our results differ from those observed by Campello-Costa et al (16). In their study, using a radioenzymatic assay and a histochemical assessment of NADPH-d activity from homogenates of the superficial layers of the superior colliculus, they showed that the maximal level of nNOS expression was at postnatal day 5 and that there was a decrease in the enzymatic activity during the following weeks reaching a significantly lower level in adulthood.…”

Section: Discussioncontrasting

confidence: 99%

“…Our results showed that during the first two postnatal weeks, the period when the final retinotopic map is established in the rat superior colliculus (39), nNOS is expressed at lower levels than in adulthood. However, the potential amount of NO synthesis by the nNOS present in this period, as assayed indirectly by the levels of enzymatic activity, seems to be sufficient to participate in the formation of this map, since previous studies with NOS inhibitors have shown that the blockage of NO synthesis impairs the refinement of retinocollicular projections (16,17).…”

Section: Discussionmentioning

confidence: 96%

“…For example, in chickens, the elimination of the ipsilateral retinotectal projections is partially dependent on the NO synthesized in the tectum opticum (14). Furthermore, several studies on rodents have suggested that NO might be involved in the topographic establishment of the ipsilateral retinotectal projections (15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

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Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus

Giraldi-Guimarães

Bittencourt-Navarrete

Mendez-Otero

2004

Braz J Med Biol Res

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We investigated the level of expression of neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of the rat superior colliculus during early postnatal development. Male and female Lister rats ranging in age between the day of birth (P0) and the fourth postnatal week were used in the present study. Two biochemical methods were used, i.e., in vitro measurement of NOS specific activity by the conversion of [ 3 H]-arginine to [ 3 H]-citrulline, and analysis of Western blotting immunoreactive bands from superior colliculus homogenates. As revealed by Western blotting, very weak immunoreactive bands were observed as early as P0-2, and their intensity increased progressively at least until P21. The analysis of specific activity of NOS showed similar results. There was a progressive increase in enzymatic activity until near the end of the second postnatal week, and a nonsignificant tendency to an increase until the end of the third week was also observed. Thus, these results indicated an increase in the amount of nNOS during the first weeks after birth. Our results confirm and extend previous reports using histochemistry for NADPH-diaphorase and immunocytochemistry for nNOS, which showed a progressive increase in the number of stained cells in the superficial layers during the first two postnatal weeks, reaching an adult pattern at the end of the third week. Furthermore, our results suggested that nNOS is present in an active form in the rat superior colliculus during the period of refinement of the retinocollicular pathway.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 96%

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Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus

Giraldi-Guimarães

Bittencourt-Navarrete

Mendez-Otero

2004

Braz J Med Biol Res

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“…This small, highly diffusible molecule, synthesized in biological tissues by a family of enzymes termed the nitric oxide synthases (NOS’s), mediates its effects principally via activation of soluble guanylyl cyclase (sGC) and cyclic guanosine monophosphate (cGMP) synthesis [9]–[11]. The NOS1 isozyme is expressed in neuronal tissue, often during periods of growth cone extension and synapse formation [1], [12]–[23] and can influence both synapse assembly [24]–[37] and maintenance [6], [8], [38], [39]. Recent evidence also suggests NO has developmental effects on the developing neuromuscular junction (NMJ): in Xenopus and chick embryos chronic NO treatment promotes acetylcholine (ACh) receptor clustering [2]–[5].…”

Section: Introductionmentioning

confidence: 99%

Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos

2014

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Nitric oxide is a bioactive signalling molecule that is known to affect a wide range of neurodevelopmental processes. However, its functional relevance to neuromuscular development is not fully understood. Here we have examined developmental roles of nitric oxide during formation and maturation of neuromuscular contacts in zebrafish. Using histochemical approaches we show that elevating nitric oxide levels reduces the number of neuromuscular synapses within the axial swimming muscles whilst inhibition of nitric oxide biosynthesis has the opposite effect. We further show that nitric oxide signalling does not change synapse density, suggesting that the observed effects are a consequence of previously reported changes in motor axon branch formation. Moreover, we have used in vivo patch clamp electrophysiology to examine the effects of nitric oxide on physiological maturation of zebrafish neuromuscular junctions. We show that developmental exposure to nitric oxide affects the kinetics of spontaneous miniature end plate currents and impacts the neuromuscular drive for locomotion. Taken together, our findings implicate nitrergic signalling in the regulation of zebrafish neuromuscular development and locomotor maturation.

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“…It has been suggested that NO is involved in many physiological processes in the adult and developing nervous system such as synaptic transmission, stabilization and plasticity (reviewed Chugani 1999;Calabresi et al 2000). Particularly during development, NO has been implicated in the formation and stabilization of specific connections (Cramer et al 1996;Renteria and Constantine-Paton 1999;Campello-Costa et al 2000;Vercelli et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Diaphorase-positive neurons in the cingulate cortex of human fetuses during the second half of gestation

deAzevedo

Hedin-Pereira²,

Lent

2002

Anatomy and Embryology

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In this work, the time course of appearance, distribution and morphology of diaphorase-positive neurons were studied in the developing cingulate cortex of the human brain during the second half of gestation. Five human fetuses at 18, 20, 25, 30 and 35 weeks postovulatory (wpo) were examined. The brain tissue was reacted by an indirect histochemistry protocol for detection of NADPH-diaphorase activity. Labeled neurons were identified at the microscope and documented photographically or by computer-aided charts. We have found that heavily labeled neurons (type I) first appear in the subplate (SP) between 20 and 25 wpo, and in the cortical plate (CP) between 25 and 35 wpo. By 35 wpo, CP neurons were both type I and type II (lightly labeled neurons). In addition, we observed 4 different morphological types among subplate neurons, very similar to callosally-projecting subplate cells (as described previously by our group). We concluded that medial nitridergic neurons of humans appear prenatally according to the usual gradient of cortical maturation -- first in the subplate and later in the cortical plate. Also, we suggest that some of the diaphorase-positive neurons in the transient subplate could possibly be callosal.

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Acute blockade of nitric oxide synthesis induces disorganization and amplifies lesion-induced plasticity in the rat retinotectal projection

Cited by 32 publications

References 54 publications

Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus

Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus

Effects of Nitric Oxide on Neuromuscular Properties of Developing Zebrafish Embryos

Diaphorase-positive neurons in the cingulate cortex of human fetuses during the second half of gestation

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