Acute and sub-chronic effects of copper on survival, respiratory metabolism, and metal accumulation in Cambaroides dauricus

Abstract

Trace metal contamination in the aquatic ecosystem occurs worldwide: although copper is an essential trace metal, it is considered as a pollutant at certain levels in China. Freshwater crayfish Cambaroides dauricus is a commercially important wild species in northeastern China, in which is an important heavy industry area. The population of C. dauricus was decreasing sharply due to the environmental pollution and human intervention over the past 20 years. However, nothing is known regarding the responses of this species to trace metal toxicants. This study aimed to determine the acute and chronic toxicity of Cu and its toxicological effects on respiratory metabolism, as well as Cu accumulation in C. dauricus. The acute (96 h) median lethal concentration (LC50) value of 32.5 mg/L was detected in C. dauricus. Then, acute (96 h; 8.24, 16.48 mg/L) and sub-chronic (14 days; 2.06, 4.12 mg/L) exposure in Cu was investigated by estimating the oxygen consumption rate, ammonium excretion rate, and Cu accumulation. Both acute and sub-chronic Cu exposure induced an inhibition of the oxygen consumption rate and ammonium excretion rate, and thereby, an increased O:N ratio. The shift in O:N ratio indicated a metabolic substrate shift towards lipid and carbohydrate metabolism under Cu stress. Cu accumulation in the hepatopancreas and muscles throughout the study was found to be time-dependent and concentration-dependent. The maximum accumulation in the hepatopancreas and muscle were almost 31.6 folds of the control after 14 days’ exposure to 4.12 mg/L concentration. Based on the present work, we suggest that crayfish be considered a potential bioindicator of environmental pollution in freshwater systems. The study provides basic information for further understanding of the toxicological responses of this species to trace metals.

Copper Tolerance Mechanism of the Novel Marine Multi-Stress Tolerant Yeast Meyerozyma guilliermondii GXDK6 as Revealed by Integrated Omics Analysis

“…A peroxisome is a type of organelle involved in fatty acid oxidation, amino acid and polyamine catabolism, and ROS reduction (Bonekamp et al, 2009). Peroxisome is a highly conserved cellular pathway that plays an important role in the selective degradation of cellular proteins, thereby helping regulate various vital cellular functions (Banfalvi, 2011). The enhanced proteasome activity is beneficial to reduce copper stress-induced protein ubiquitination and carbonylation (Romero-Puertas et al, 2004), which is advantageous for GXDK6 to survive under high-copper concentrations.…”

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Prognosis Prediction Based on Cuproptosis-Related lncRNAs and Immune Responses in Patients with LUAD

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Evidence-based meta-analysis of the genotoxicity induced by microplastics in aquatic organisms at environmentally relevant concentrations

“…The publication bias was examined by Egger's test (Egger et al, 1997) were used as the limits of grouping, which could ensure that each group included 8 or more effect sizes. Regarding the exposure duration, a total of 9 different exposure periods were included, which could be divided into three groups (each group involving 9 or more effect sizes), including groups of acute exposure (1, 3 and 4 days), sub-acute exposure (5, 7 and 10 days) and sub-chronic (14, 15 and 26 days) exposure (Bao et al, 2020). In terms of the particle size, the data could be divided into two groups (each group included more than 10 effect sizes), and the grouping limit was set at 1 μm that was also the size limit between nanoplastics (NPs) and large MPs (LMPs) (Gigault et al, 2018).…”