Acute and sub-acute Toxicity study of Aqueous extracts of Canscora heteroclita (L) Gilg in Rodents

Rajasekaran, A.; Arivukkarasu, Ramasamy

doi:10.5530/pj.2016.4.15

Cited by 8 publications

(4 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Normal food and water consumption of animals support the gain in the body weight of animals throughout the study period. Similar findings were also observed in this study as no significant reduction in the body weight was noted in any group of the animals 21 . Moreover no significant difference was observed in relative weight of any organ (viz.…”

Section: Discussionsupporting

confidence: 92%

Research article Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods

Ahmad

Wadud²,

Jahan³

et al. 2021

J. Drug Delivery Ther.

View full text Add to dashboard Cite

Background: Though a number of studies has been carried out in terms of safety of Kushta but concerns have been raised about the presence of heavy metals in Unani formulations. Classical method of preparation of KKH and other Kushta preparation in Unani system of medicine has been found to eliminate all such issues from the end product. Hence; the present study was designed to evaluate the acute and sub-acute toxicity study of KKH prepared by Classical and Muffle furnace methods. Objective: To ascertain the safety profile of Kushta Khabasul Hadeed (KKH) prepared by classical and muffle furnace methods. Materials and methods: Both of the samples of KKH i.e. Kushta Khabasul Hadeed (KKHCM) and Kushta Khabasul Hadeed (KKHMFM) prepared by Classical and Muffle Furnace Methods respectively were evaluated for acute and sub-acute toxicity by OECD guidelines 423 and 407 respectively. The starting dose in the acute test was 50 mg/kg and in sub-acute test, it was 134mg, 200 mg and 400 mg/kg b.w. Effect of both of the test samples were assessed for ponderal changes, food and water intake, relative organs weights, hematological, biochemical and histopathological features of various organs. Results: Both of the samples did not produce any sign of toxicity in acute toxicity study up to the dose level of 2000mg/kg b.w. In sub-acute toxicity study no mortality in any of the test groups was observed. KKHCM at different doses showed a slight decrease in food intake in all the test groups but significant difference (P<0.05) was observed in the test group B as compared to plain control. Conclusion: KKHMFM was found to be relatively toxic than KKHCM at different dose levels. The study demonstrated that both the Classical and Muffle furnace methods of preparation of Kushta are useful for preparing KKH and also safe at the dose prescribed in Unani literature. However, the Kushta prepared by the classical method was found to be safer than the muffle furnace method.

show abstract

Section: Discussionsupporting

confidence: 92%

Research article Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods

Ahmad

Wadud²,

Jahan³

et al. 2021

J. Drug Delivery Ther.

View full text Add to dashboard Cite

show abstract

“…Although acute toxicity helps to determine the toxicity nature of substances, it is important to assess subacute oral toxicity profile because it helps to evaluate the morphological and physiological changes in organs after repeated administration [33, 34]. In oral toxicity assessment the body weight change is sensitive and indicative marker for toxicity sign [35]. No significant changes in the body weight were observed in treated mice compared to untreated group after 21 days administration of L. angustifolia EO.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice

Mekonnen

Tesfaye

Christos

et al. 2019

Journal of Toxicology

View full text Add to dashboard Cite

Lavandula angustifolia is used in traditional and folk medicines of Ankober District, North Central Ethiopia, for the treatment of several livestock and human disorders. This toxicity study aimed to investigate L. angustifolia essential oil oral toxicity in mice and skin irritation in rabbit. L. angustifolia essential oil was analyzed using gas chromatography-mass spectrometry methods and showed predominance of Eucalyptol (52.36%), Camphor (11.91%), gamma-terpinene (8.775%) and endoborneol (7.585%). Limit test at 2000 mg/kg dose was used for L. angustifolia essential oil acute toxicity test and revealed LD50 value was higher than 2000 mg/kg. For subacute toxicity study 2000mg/kg was given orally to each mouse for 21 days. The result demonstrated no significant changes (p > 0.05) in the body weights, and biochemical parameters, gross abnormalities, water, and food intake were observed. No macroscopic changes were seen in the histopathology analysis of kidneys and livers. For skin irritation test shaved rabbit skin was treated with 10% ointment formulation. Ointment of L. angustifolia oil did not affect mice skin. Generally, this toxicity study demonstrated that L. angustifolia essential oil is nontoxic.

show abstract

“…This may be due to the fact that the extract BERM, may contains some nutritive substances which led the animals to be active. On the contrary, the results obtained by [33] clearly suggested that there is an increase in the body weight of the animals was observed. In terms of organ weight ratio, the results found to be normal and all within the range.…”

Section: Discussionmentioning

confidence: 82%

HPLC characterization, acute and sub-acute toxicity evaluation of bark extract of Rhizophora mucronata in Swiss Albino mice

Jairaman¹,

Yacoob²,

Sivanesan

et al. 2020

Heliyon

View full text Add to dashboard Cite

Aim: Rhizophora mucronata, commonly called as 'red mangrove' grows in the tropical and subtropical regions and on the sheltered shores. The bioactive compounds from the plant have been used in the treatment of wide range of diseases. Though the beneficial effects have been reported, the safety and toxicological studies are not carried out. Hence, major bioactives have been identified by HPLC and then acute and sub-acute toxicity studies of (BERM) in Swiss Albino mice have been carried out. Main methods: HPLC fingerprinting was carried out of BERM for the characterization of bioactives. BERM as a single dose was given orally at 800, 1600 mg/kg and 3200 mg/kg by a stainless steel cannula to the mice. Then the mice were observed for 14 days for mortality and behavioural changes. Food, water intake and body weight changes were also observed throughout the study period. On the fifteenth day, the mice were anesthetized with isofluorane and blood was withdrawn for haematological and biochemical analysis. The animals were sacrificed by overdose of isofluorane and organs such as liver, kidney, lungs and spleen were dissected out for histopathological analysis. There was no mortality of the mice even in 3200 mg/kg dose, stating that the oral LD50 of BERM is more than 5000 mg/kg. In terms of Sub-acute toxicity, for a period of 28 days repeated dose of 400 mg/ kg and 800 mg/kg as an optimum dose and a control group was kept with only distilled water at 5 ml/kg against the treated groups. On 29 th day, the mice from all groups were sacrificed and blood was withdrawn and organs such as liver, kidney, lungs and spleen were dissected out for the assessment of internal tissues, wherein no abnormalities were observed in the treatment groups as compared to the control. The blood parameters, biochemical analysis of the treated groups were well within the range, histopathological confirmed the findings wherein the organs viz, liver, kidneys, lungs and spleen possessed normal architecture. Key findings: Based on HPLC results, prominent 5 major compounds viz: Diadzein, Epicatechin, Hesperidin, Diosmin and Quercitrin respectively were identified. Isolated changes observed in the haematological, biochemical and histopathological studies were not dose related and showed the safety of the bark extract. Similarly, the sub-acute toxicity of BERM has been conducted for 28 days, wherein repeated dose of 400 mg/kg and 800 mg/kg and control group was given orally. There were no abnormalities found both in external and internal parameters. Significance: Based on the study it is concluded that the bark extract of Rhizophora mucronata (BERM) is safe at 1000 mg/kg or less on repeated dosage can be considered as a safe dose for pharmacological efficacy studies.

show abstract

Acute and sub-acute Toxicity study of Aqueous extracts of Canscora heteroclita (L) Gilg in Rodents

Cited by 8 publications

References 7 publications

Research article Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods

Research article Toxicity Studies on Kushta Khabasul Hadeed (calcined iron rust) prepared by Classical and Muffle Furnace Methods

Evaluation of Skin Irritation and Acute and Subacute Oral Toxicity of Lavandula angustifolia Essential Oils in Rabbit and Mice

HPLC characterization, acute and sub-acute toxicity evaluation of bark extract of Rhizophora mucronata in Swiss Albino mice

Contact Info

Product

Resources

About