Acute and sub-acute toxicity study of a Pakistani polyherbal formulation

Ishtiaq, Saiqa; Akram, Maida; Kamran, Sairah Hafeez; Hanif, Uzma; Afridi, Muhammad Shaharyar Khan; Sajid-ur-Rehman,; Afzal, Atika; Asif, A. K.; Younus, Muhammad; Akbar, Shehla

doi:10.1186/s12906-017-1889-7

Cited by 22 publications

(12 citation statements)

References 32 publications

(21 reference statements)

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“…The toxic effect of the PHF5 extract may be attributed to the presence of one or more phyto-compounds, which have a toxic impact on the animal model. Similar results were found in an acute and sub-acute toxicity study of a Pakistani polyherbal formulation [30,31] and in an investigation of Chinese traditional medicine when an herbal formula was used in patients. However, liver toxicity was attributed to overdosing [32].…”

Section: Resultssupporting

confidence: 82%

Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5

et al. 2020

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AbstractA popular polyherbal formulation prepared from five plants (PHF5) may have anticancer effects. However, there is a lack of adequate scientific evidence. We assessed the anticancer, antioxidant, and acute toxicity effects of PHF5. Cancer cells were treated with 0 to 300 μg/mL PHF5 extract. Established assays were used to assess cytotoxicity, apoptosis, and radical scavenging activities. In the acute toxicity study, mice were administered a single oral dose (2,000 mg/kg) of PHF5, and biochemical and histopathological parameters were assessed. The IC50 values of PHF5 on LoVo, HepG2, MCF-7, and MDA-MB 231 cells were 71.8, 64.8, 45.3, and 47.3 μg/mL, respectively. Fluorescence staining demonstrated that PHF5 induced MCF-7 cell apoptosis. After 48 h, the percentage of late apoptotic cells increased significantly compared with the control cells (74.16 ± 0.64 vs 3.7 ± 2.05, P < 0.05). No mortality or behavioral alterations were observed in mice treated with a single dose (2,000 mg/kg) of PHF5, indicating that the LD50 value exceeded 2,000 mg/kg. However, histopathological changes were observed in the liver tissues. PHF5 has potential as a therapeutic agent for the treatment of human carcinoma. Further safety data will be necessary before clinical use.

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Section: Resultssupporting

confidence: 82%

Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5

et al. 2020

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“…As a result, extensive studies are underway to evaluate the development and use of traditional medicines in advancing their effectiveness and safety [ 27 ]. Nonetheless, inadequate scientific data on dosing, quality, toxicity, safety and efficacy of various traditional medicine formulations are available [ [27] , [28] , [29] ]. Toxicity testing is vital as it facilitates the evaluation of the possible harmful side effects attributed to these products.…”

Section: Discussionmentioning

confidence: 99%

Acute toxicity studies of the South African medicinal plant Galenia africana

2018

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“…The tissues were then stained with eosin and hematoxylin. The structural changes in prepared liver slides were analyzed under microscope [17].…”

Section: Histopathological Examinationmentioning

confidence: 99%

Evaluation of anti-nociceptive, anti-inflammatory and hepatoprotective effects of methanol extract of Mazus pumilus (Burm. f.) Steenis (Mazaceae) herb

Ishtiaq¹,

Ilyas²,

Irshad³

et al. 2021

Trop. J. Pharm Res

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Purpose: This study was designed to investigate the anti-nociceptive, anti-inflammatory and hepatoprotective activities of the methanol extract of Mazus pumilus (Mazaceae) herb. Methods: Anti-nociceptive activity was determined using hot plate, tail flick and acetic acid-induced writing methods. Carrageenan-induced rat paw edema (0.1 mL of 1 %) model was used for the assessment of anti-inflammatory activity. The methanol extract was administered orally at three different doses (150, 300 and 600 mg/kg) to three separate groups in all the experiments. Diclofenac sodium (50 mg/kg) was used as standard drug while control group received DMSO (1 %, 10 mL/kg). The hepatocurative effect of methanol extract of M. pumilus (400 mg/kg) was determined in isoniazid (50 mg/kg) and rifampicin (100 mg/kg) induced liver injury. Silymarin (100 mg/kg) was used as standard drug for comparison. The control group received distilled water (10 mL/kg). Preliminary phytochemical screening was also carried out. Results: The methanol extract of M. pumilus significantly (p < 0.05) augmented latency time and reduced the number of writhes in the pain models at all doses used for the assessment of antinociceptive actions. The anti-inflammatory activity of different doses of extract was evaluated by measuring the reduction in the size of the paw. A significant (p < 0.05) hepatocurative effect was observed when administered after anti-tuberculosis drugs. Histopathological analysis of the liver tissues also revealed restored hepatocellular architecture. Conclusion: The results demonstrate the anti-nociceptive, anti-inflammatory and hepatoprotective effects of the methanol extract of M. pumilus, thus substantiating the ethnomedical claims associated with the herb.

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Acute and sub-acute toxicity study of a Pakistani polyherbal formulation

Cited by 22 publications

References 32 publications

Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5

Anticancer, antioxidant, and acute toxicity studies of a Saudi polyherbal formulation, PHF5

Acute toxicity studies of the South African medicinal plant Galenia africana

Evaluation of anti-nociceptive, anti-inflammatory and hepatoprotective effects of methanol extract of Mazus pumilus (Burm. f.) Steenis (Mazaceae) herb

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