Acute and repeated-doses (28 days) toxicity study of Hypericum polyanthemum Klotzsch ex Reichardt (Guttiferare) in mice

Betti, Andresa Heemann; Stein, Ana Cristina; Dallegrave, Eliane; Wouters, Angélica Terezinha Barth; Watanabe, Tatiane Terumi Negrão; Driemeier, D.; Buffon, Andréia; Rates, Stela Maris Kuze

doi:10.1016/j.fct.2012.04.012

Cited by 24 publications

(15 citation statements)

References 33 publications

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“…The oral toxicity of this formulation can be classified in category 5 (the lethal acute toxicity is greater than 5000 mg/kg) according to the Globally Harmonized Classification System of OECD [26]. …”

Section: Resultsmentioning

confidence: 99%

Toxicity Profile of a Nutraceutical Formulation Derived from Green MusselPerna viridis

Chakraborty

Joseph

Chakkalakal

2014

BioMed Research International

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The short-term (acute) and long-term (subchronic) toxicity profile, mean lethal dose 50 (LD50), and no-observed-adverse-effect level (NOAEL) of a nutraceutical formulation developed from green mussel Perna viridis, which showed in vitro and in vivo anti-inflammatory properties, were evaluated in the present study. The formulation was administered to the male and female Wistar rats at graded doses (0.5, 1.0, and 2.5 g/kg body weight) for two weeks of acute toxicity study and 0.5, 1.0, and 2.0 g/kg body weight for 90 days in subchronic toxicity study. The LD50, variations in clinical signs, changes in body weight, body weight, food/water consumption, organ weight (liver, kidney, spleen, and brain), hematology, serum chemistry, and histopathological changes were evaluated. The LD50 of the formulation was 5,000 mg/kg BW. No test article related mortalities as well as change in body weight, and food and water consumption were observed. No toxicity related significant changes were noted in renal/hepatic function, hematological indices, and serum biochemical parameters between the control and treated groups. Histopathological alterations were not observed in the vital organs of rats. The subchronic NOAEL for the formulation in rats is greater than 2000 mg/kg. This study demonstrated that the green mussel formulation is safe to consume without any adverse effects in the body.

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Section: Resultsmentioning

confidence: 99%

Toxicity Profile of a Nutraceutical Formulation Derived from Green MusselPerna viridis

Chakraborty

Joseph

Chakkalakal

2014

BioMed Research International

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“…Bodyweight change is used as a general indicator of the adverse effects of chemicals on a living organism [ 6 ]. Thus, weight loss is correlated with the physiological condition of the animal and can be explained not only by anorexia [ 50 ] but also by impaired metabolism in animals [ 51 ]. These data indicate that R. coccinea may have adverse effects on animal metabolism.…”

Section: Discussionmentioning

confidence: 99%

Larval Cytotoxic and Subacute Toxicity of Gardenia ternifolia, Rourea coccinea, and Cassytha filiformis Used in Traditional Medicine of Benin (West Africa)

Agbodjento

Klotoé

Sacramento

et al. 2020

Journal of Toxicology

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The use of medicinal plants in traditional medicine is a common practice in developing countries. However, this unregulated or irrational use may pose a risk of toxicity to humans in the short and/or long term. Recent studies reported interesting ethnopharmacological, antioxidant, and phytochemical data on some medicinal plants used in the traditional treatment of male infertility in Benin. Unfortunately, very little data exist on the long-repeated dose toxicity of these medicinal plants. This study was aimed at evaluating the larval cytotoxicity and subacute toxicity of the hydroethanolic extract of Cassytha filiformis whole plant, Gardenia ternifolia roots, and Rourea coccinea leaves. The subacute toxicity of these plants was evaluated in male Wistar albino rats at three different doses (200, 400, and 800 mg/kg) according to the OECD 407 guidelines. Hematological and biochemical examinations and the histological study of the liver and kidneys were carried out. Larval cytotoxicity was assessed by the sensitivity of Artemia salina larvae to different concentrations of the studied plants extracts. The mean lethal concentration (LC50) was determined by the probit method. Subacute toxicity data indicated that there was no mortality or structural alterations of the liver and kidneys in the lot of treated animals. However, significant alterations in certain hematological and biochemical parameters (hematocrit, ASAT, and uremia) were noted. These abnormalities were observed in the lot of rats treated with Rourea coccinea and Cassytha filiformis extracts. Larval cytotoxicity data indicate that the studied plants extracts are not cytotoxic (LC50 > 0.1 mg/mL). These data suggest that the use in traditional medicine of studied plants at high doses and repeated over a long period of time requires special attention.

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“…11,12 In the acute toxicity study, dosages upto1,000 mg/kg did not exhibit any mortality or any signs of toxicity (e.g., changes in body weight and the sum of food consumption) after oral administration of a single dose (Figure 1). This dosage might be considered as the no observed adverse effect level (NOAEL) 8 for DPSUU III.…”

Section: Resultsmentioning

confidence: 99%

“…As seen in (Figure 6), DPSUU III uniquely suppressed proliferation of MCF-7 cells at 250 and 500 μg/mL ( Figure 6) (p< 0.05 vs. control).0. 8,11,12 To explain the mechanism of cell growth inhibition by the DPSUU III plant complex, we determined its effect on cell cycle distribution. Exposure of MCF-7 cells to the DPSUU III plant complex resulted in the improvement of the G0-G1 fraction, which was accompanied by a decrease in the S phase ( Figure 7).…”

Section: Resultsmentioning

confidence: 99%

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Pharmacognostic Evaluation of DPSUU III IN Breast Tumor Growth By G0/G1 Cell Cycle Arrest

Pradhan¹,

Dasmohapatra²,

Tripathy³

2017

IJPER

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Objective: Plant-inferred bioactive particles have been utilized to turn away or treat tumor for a considerable length of time. As of late plant blends were found to have significantly more grounded exercises than utilizing plant alone. In this study, we evaluated the anticancer impacts of DPSUU III which contains a mix of plant concentrates of Beta vulgaris, Syzygium cumini, Limonia acidissima. Material and Methods: After extraction from these plants, DPSUU III was granulated and powder was extricated with 80% ethanol, dried by rotating evaporator under vacuum and put away until utilize. DPSUU III was offered orally to mice at single measurements of 1000mg/kg/day alongside vehicle control and creatures were seen amid the initial 12 hr for any change in side effects of mobility, posture, amount of sustenance utilization and for mortality after MCF-7 cells were infused into mammary cushion of C57BL6 mice to create strong breast tumours. Then DPSUU III treated gathering was contrasted with the doxorubicin (Dox)-treated gathering. Results: The breast disease development was altogether stifled in mice treated with DPSUU III without loss of body weight and any symptoms while Dox-treated mice showed huge body weight reduction with little measure of toxicities. An expansion in life expectancy (ILS% = 62.80%) was seen in the DPSUU III -controlled gathering, contrasted with the tumor control bunch. DPSUU III shows hostile to proliferative movement against MCF-7 breast tumor cells through actuating G0/G1 cell cycle capture. DPSUU III hinders the declaration of cyclin D1, CDK4, CDK2 and prompts p21. Conclusion: DPSUU III could be a potential chemo preventive supplement to breast tumor patients.

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Acute and repeated-doses (28 days) toxicity study of Hypericum polyanthemum Klotzsch ex Reichardt (Guttiferare) in mice

Cited by 24 publications

References 33 publications

Toxicity Profile of a Nutraceutical Formulation Derived from Green MusselPerna viridis

Toxicity Profile of a Nutraceutical Formulation Derived from Green MusselPerna viridis

Larval Cytotoxic and Subacute Toxicity of Gardenia ternifolia, Rourea coccinea, and Cassytha filiformis Used in Traditional Medicine of Benin (West Africa)

Pharmacognostic Evaluation of DPSUU III IN Breast Tumor Growth By G0/G1 Cell Cycle Arrest

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