Acute and chronic exposure of Dunaliella salina and Chlamydomonas bullosa to copper and cadmium: Effects on ultrastructure

Visviki, I.; Rachlin, Joseph W.

doi:10.1007/bf00224799

Cited by 27 publications

(36 citation statements)

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“…It tolerated a Cd concentration as high as 40 mg Cd 2þ /L along 96 h of culture, and the EC50 value obtained was 48.9 mg Cd/L. However, a study was performed by Visviki and Rachlin [23] in which the EC50 obtained for the same microalga was much lower, although the culture conditions were completely different.…”

Section: Discussionmentioning

confidence: 93%

Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds

Suárez

Torres

Pérez-Rama

et al. 2010

Enviro Toxic and Chemistry

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Cadmium (Cd) toxicity and production of different thiols (phytochelatins, glutathione, gamma-Glu-Cys and cysteine) were studied in the microalga Chlamydomonas moewusii exposed to different concentrations of this metal (1, 2, 4, 6, 8, and 10 mg/L) for 96 h. The inhibitory effect of Cd on growth was demonstrated. The value of EC50 (metal concentration which reduces the population growth to 50% of the control) obtained for this microalga was estimated at 4.1 +/- 0.8 mg/L of Cd after 96 h of exposure. The amount of thiol compounds synthesized by C. moewusii changed with Cd concentration. Cysteine concentrations were significantly higher compared to those of gamma-Glu-Cys and glutathione in all the Cd concentrations assayed. The amino acid cysteine reached its higher levels in those cultures in which a decrease in the concentration of phytochelatins (PCs) was observed. Both cysteine and glutathione concentrations showed significant differences along the Cd concentrations assayed, while the amount of gamma-Glu-Cys detected remained stable. The PCs detected were of two, three, and four subunits. The level of PC(2) was higher than that of PC(3) and PC(4). PC(4) was detected only in the cultures exposed to the Cd concentrations of 1 and 2 mg/L, in which the synthesis of phytochelatins was higher. A rapid increase in the production of PC(2) and PC(3) was observed up to a Cd concentration of 2 mg/L, after which their levels began to decrease. Phytochelatins were not detected in cultures without Cd (controls) and in those exposed to the maximum Cd concentration (10 mg/L), in which cell growth was completely inhibited.

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Section: Discussionmentioning

confidence: 93%

Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds

Suárez

Torres

Pérez-Rama

et al. 2010

Enviro Toxic and Chemistry

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“…We showed that in tests 44 with P. antarctica, exponential growth in the control treatments was still occurring up 45 to 10 days (Supplemental data, Figure S2), indicating that nitrate and phosphate 46 were ample in the test solutions.…”

mentioning

confidence: 97%

A robust bioassay to assess the toxicity of metals to the Antarctic marine microalga Phaeocystis antarctica

Gissi

Adams

King

et al. 2015

Enviro Toxic and Chemistry

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. (2015). A robust bioassay to assess the toxicity of metals to the antarctic marine microalga Phaeocystis antarctica. Environmental Toxicology and Chemistry, 34 (7), 1578-1587. A robust bioassay to assess the toxicity of metals to the antarctic marine microalga Phaeocystis antarctica AbstractDespite evidence of contamination in Antarctic coastal marine environments, no water-quality guidelines have been established for the region because of a paucity of biological effects data for local Antarctic species. Currently, there is limited information on the sensitivity of Antarctic microalgae to metal contamination, which is exacerbated by the lack of standard toxicity testing protocols for local marine species. In the present study, a routine and robust toxicity test protocol was developed using the Antarctic marine microalga Phaeocystis antarctica, and its sensitivity was investigated following 10-d exposures to dissolved copper, cadmium, lead, zinc, and nickel. In comparisons of 10% inhibition of population growth rate (IC10) values, P. antarctica was most sensitive to copper (3.3 μg/L), followed by cadmium (135 μg/L), lead (260 μg/L), and zinc (450 μg/L). Although an IC10 value for nickel could not be accurately estimated, the no-observed-effect concentration value for nickel was 1070 μg/L. Exposure to copper and cadmium caused changes in internal cell granularity and increased chlorophyll a fluorescence. Lead, zinc, and nickel had no effect on any of the cellular parameters measured. The present study provides valuable metal-ecotoxicity data for an Antarctic marine microalga, with P. antarctica representing one of the most sensitive microalgal species to dissolved copper ever reported when compared with temperate and tropical species. ABSTRACT Despite evidence of contamination in Antarctic coastal marine environments, no water quality guidelines have been established for the region due to a paucity of biological effects data for local Antarctic species. Currently there is limited information on the sensitivity of Antarctic microalgae to metal contamination, which is exacerbated by the lack of standard toxicity testing protocols for local marine species. In the present study, a routine and robust toxicity test protocol was developed using the Antarctic marine microalga Phaeocystis antarctica, and its sensitivity investigated following 10-d exposures to dissolved copper, cadmium, lead, zinc, and nickel. In comparisons of IC10 (10% inhibition of population growth rate) values, P. antarctica was most sensitive to copper (3.3 µg/L), followed by cadmium (135 µg/L), lead (260 µg/L) and zinc (450 µg/L). While an IC10 value for nickel could not be accurately estimated, the NOEC value for nickel was 1070 µg/L. Exposure to copper and cadmium caused changes in internal cell granularity, and increased chlorophyll a fluorescence. Lead, zinc and nickel had no effect on any of the cellular parameters measured. The present study provides valuable metal-ecotoxicity data for an Antarctic marine microalga, with P. antarctica re...

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“…The increase of cytoplasmic membranes observed in the CuNi treatment may be related to cation excess. At sublethal concentrations, the lipids and proteins of these membranes may tie up excess quantities of intracellular cations, as suggested earlier by Visviki and Rachlin (1994) in the case of copper-exposed Chlamydomonas bullosa cells and by Rachlin et al (1982) in the case of copper-and nickel-exposed Plectonema boryanum cells, though this mechanism has not been studied in higher plants.…”

Section: Discussionmentioning

confidence: 78%

Long-Term Symptoms Due to Metal and Acid Precipitation Treatments in Scots Pine (Pinus sylvestris) Needles in the Subarctic

Kukkola¹,

Rautio²,

Huttunen³

2005

Arctic, Antarctic, and Alpine Research

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Acute and chronic exposure of Dunaliella salina and Chlamydomonas bullosa to copper and cadmium: Effects on ultrastructure

Cited by 27 publications

References 0 publications

Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds

Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds

A robust bioassay to assess the toxicity of metals to the Antarctic marine microalga Phaeocystis antarctica

Long-Term Symptoms Due to Metal and Acid Precipitation Treatments in Scots Pine (Pinus sylvestris) Needles in the Subarctic

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