Long-Term Symptoms Due to Metal and Acid Precipitation Treatments in Scots Pine (Pinus sylvestris) Needles in the Subarctic

Kukkola, Eija; Rautio, Pasi; Huttunen, Satu

doi:10.1657/1523-0430(2005)037[0068:lsdtma]2.0.co;2

Cited by 2 publications

(3 citation statements)

References 24 publications

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“…Similarly, as reported by Masuch et al (1992), the volume of intercellular spaces per mesophyll cell volume increased after acid rain application. By contrast, Kukkola et al (2005), who assessed the proportion of intercellular spaces using the point-counting method on semi-thin sections of Scots pine needles treated with simulated acid rain, found no effect of SAR on the extent of intercellular spaces. Moss et al (1998), however, detected large cavities in red spruce mesophyll caused by the acid mist event, which corresponds to the present finding of increased volume of intercellular spaces after the SAR treatment.…”

Section: Discussionmentioning

confidence: 91%

“…Conifer needle anatomy has been predominantly studied for Norway spruce since the 1980s, mainly due to the widespread forest decline caused by atmospheric pollution and acid rain (Albrechtová et al, 2001). However, most studies describe the anatomical alterations only qualitatively, for example, by the presence of collapsed cells (Kukkola et al, 2005) or loss of chloroplasts (Moss et al, 1998). In the present study, the purpose was to test if the confocal stereology analysis is capable of capturing subtle changes in mesophyll arrangement.…”

Section: Discussionmentioning

confidence: 99%

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Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles

Albrechtová

Janáček

Lhotáková

et al. 2007

Journal of Experimental Botany

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Recent design-based stereological methods that can be applied to thick sections cut in an arbitrary direction are presented and their implementation for measuring mesophyll anatomical characteristics is introduced. These methods use software-randomized virtual 3D probes, such as disector and fakir test probes, in stacks of optical sections acquired using confocal microscopy. They enable unbiased estimations of the mean mesophyll cell volume, mesophyll cell number in a needle, and for the first time an internal surface area of needles or other narrow leaves directly from the fresh tissue cross-sections cut using a hand microtome. Therefore, reliable results can be obtained much faster than when using a standard microtechnical preparation. The proposed methods were tested on Norway spruce needles affected for 1 year by acid rain treatment. The effect of acid rain resulted in changes of mesophyll parameters: the ratio of intercellular spaces per mesophyll cell volume increased, while needle internal surface area, total number of mesophyll cells, and number of mesophyll cells per unit volume of a needle decreased in the treated needles.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles

Albrechtová

Janáček

Lhotáková

et al. 2007

Journal of Experimental Botany

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“…Using this approach, the volume density of individual tissues in a leaf was measured by Edwards et al (1999), Klich (2000), Luković et al (2001), Bray and Reid (2002), Luković (2006), Marin et al (2006), Zorić et al (2011;, Moura and Alves (2014), Bernardo et al (2017), and Bertel et al (2017). The proportion of intercellular spaces in mesophyll was also investigated by Albrechtová and Kubínová (1991), Kukkola et al (2005), Albrechtová et al (2007), Lhotáková et al (2008), Psaras (2011), andZorić et al (2011;. The proportion of intercellular spaces in the palisade parenchyma was measured by Konoplyova et al (2008) from micrographs of paradermal sections of the leaves.…”

Section: Plant Tissue Volume Densitymentioning

confidence: 99%

Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

Kubínová¹,

Radochová²,

Lhotáková³

et al. 2017

Image Anal Stereol

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This review presents an historical overview of stereological methods used for the quantitative evaluation of plant anatomical and cytological structures. It includes the origins of these methods up to the most recent developments such as the application of stereology based on 3D images. We focus especially on leaf, as the vast majority of studies of plant microscopic structure examine this organ. An overview of plant cell ultrastructure measurements as well as plant anatomical characteristics (e.g., plant tissue volume density, internal leaf surface area, number and mean size of mesophyll cells and chloroplast number), which were estimated by stereological methods most frequently, is presented. We emphasize the importance of proper sampling needed for unbiased measurements. Furthermore, we mention other methods used for plant morphometric studies and briefly discuss their relevance, precision, unbiasedness and efficiency in comparison with unbiased stereology. Finally, we discuss reasons for the sparse use of stereology in plant anatomy and consider the future of stereology in plant research.

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Long-Term Symptoms Due to Metal and Acid Precipitation Treatments in Scots Pine (Pinus sylvestris) Needles in the Subarctic

Cited by 2 publications

References 24 publications

Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles

Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles

Stereology, an Unbiased Methodological Approach to Study Plant Anatomy and Cytology: Past, Present and Future

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