Acupuncture stimulation of ST‐36 (Zusanli) significantly mitigates acute lung injury in lipopolysaccharide‐stimulated rats

Huang, Cheng‐Han; Huang, Chun Jen; Tsai, Pei‐Shan; Li, Yan; Xu, Haiyan

doi:10.1111/j.1399-6576.2006.01029.x

Cited by 25 publications

(28 citation statements)

References 33 publications

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“…27,28 Animal studies have also indicated that acupuncture pretreatment has protective effects against endotoxin-induced acute lung and kidney injuries. 29,30 However, little is known about its biologic basis.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture Improves Survival in Rats with Lethal Endotoxemia via the Autonomic Nervous System

Song¹,

Cao

et al. 2012

Anesthesiology

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Background: Recent advances have indicated a complex interplay between the autonomic nervous system and the innate immune system. Targeting neural networks for the treatment of sepsis is being developed as a therapeutic strategy. Because electroacupuncture at select acupoints can modulate activities of the autonomic nervous system, we tested the hypothesis that electroacupuncture at specific acupoints could modulate systemic inflammatory responses and improve survival via its impact on the autonomic nervous system in a rat model of sepsis. Methods: Sprague-Dawley male rats received electroacupuncture for 45 min before and at 1, 2, or 4 h after a lethal dose of intraperitoneal lipopolysaccharide injection (6 mg/ kg). Outcomes included survival and systemic cytokine re-

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Section: Discussionmentioning

confidence: 99%

Electroacupuncture Improves Survival in Rats with Lethal Endotoxemia via the Autonomic Nervous System

Song¹,

Cao

et al. 2012

Anesthesiology

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“…To determine the nitric oxide (NO) concentrations in collected samples, we chose to measure the sum of the stable NO metabolites, the nitrite concentration and the nitrate concentration using chemiluminescence as described previously [20] . The TNF-α, IL-1β, and IL-6 concentrations were measured using a commercially available enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturers' instructions (R&D systems, Minneapolis, MN, USA).…”

Section: Determination Of Pulmonary Nitrite/nitrate Tumor Necrosis Fmentioning

confidence: 99%

Inhibition of hypoxia inducible factor-1α ameliorates lung injury induced by trauma and hemorrhagic shock in rats

Hong

Huang

et al. 2012

Acta Pharmacol Sin

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Aim: Ischemia/reperfusion is an initial triggering event that leads to gut-induced acute lung injury (ALI). In this study, we investigated whether hypoxia inducible factor-1α (HIF-1α) played a role in the pathogenesis of lung injury induced by trauma and hemorrhagic shock (T/HS). Methods: Male Wistar rats underwent laparotomy and hemorrhagic shock for 60 min. Sham-shock animals underwent laparotomy but without hemorrhagic shock. After resuscitation for 3 h, the rats were sacrificed. Morphologic changes of the lungs and intestines were examined. Bronchoalveolar lavage fluid (BALF) was collected. Lung water content, pulmonary myeloperoxidase (MPO) activity and the levels of malondialdehyde (MDA), nitrite/nitrate, TNF-α, IL-1β, and IL-6 in the lungs were measured. The gene expression of pulmonary HIF-1α and iNOS, and HIF-1α transcriptional activity in the lungs were also assessed. The apoptosis in the lungs was determined using TUNEL assay and cleaved caspase-3 expression. Results: Lung and intestinal injuries induced by T/HS were characterized by histological damages and a significant increase in lung water content. Compared to the sham-shock group, the BALF cell counts, the pulmonary MPO activity and the MDA, nitrite/nitrate, TNF-α, IL-1β, and IL-6 levels in the T/HS group were significantly increased. Acute lung injury was associated with a higher degree of pulmonary HIF-1α and iNOS expression as well as apoptosis in the lungs. Intratracheal delivery of HIF-1α inhibitor YC-1 (1 mg/kg) significantly attenuated lung injury, and reduced pulmonary HIF-1α and iNOS expression and HIF-1α transcriptional activity in the T/HS group. Conclusion: Local inhibition of HIF-1α by YC-1 alleviates the lung injury induced by T/HS. Our results provide novel insight into the pathogenesis of T/HS-induced ALI and a potential therapeutic application.

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“…The acupuncture protocols were developed according to previously published protocols (3,5,9,10). Rats were lightly immobilized using special cages and handling to minimize stress.…”

Section: Acupuncture Protocolsmentioning

confidence: 99%

“…Rats were lightly immobilized using special cages and handling to minimize stress. Manual acupuncture stimulation of ST36 was performed by inserting a pair of stainless steel pins 0.3 mm diameter with a depth of 1.5 mm into the bilateral ST36 acupoint (acupuncture point) (3,5,9,10). The ST36 acupoint was identified at the belly of the tibialis cranialis muscle below the cranial crest of the tibia (3,5,9,10).…”

Section: Acupuncture Protocolsmentioning

confidence: 99%

Acupuncture Stimulation of ST36 (Zusanli) Attenuates Acute Renal but Not Hepatic Injury in Lipopolysaccharide-Stimulated Rats

Huang¹,

Tsai²,

Wang³

et al. 2007

Anesthesia & Analgesia

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Acupuncture stimulation of ST‐36 (Zusanli) significantly mitigates acute lung injury in lipopolysaccharide‐stimulated rats

Abstract: Acupuncture stimulation of ST-36 may be effective as a prophylaxis measure against sepsis. However, results from this study do not support the use of acupuncture for the treatment of sepsis.

Cited by 25 publications

References 33 publications

Electroacupuncture Improves Survival in Rats with Lethal Endotoxemia via the Autonomic Nervous System

Electroacupuncture Improves Survival in Rats with Lethal Endotoxemia via the Autonomic Nervous System

Inhibition of hypoxia inducible factor-1α ameliorates lung injury induced by trauma and hemorrhagic shock in rats

Acupuncture Stimulation of ST36 (Zusanli) Attenuates Acute Renal but Not Hepatic Injury in Lipopolysaccharide-Stimulated Rats

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