Activity of Δ5-3β-Hydroxysteroid dehydrogenase, succinate dehydrogenase, and glucose-6-phosphate dehydrogenase in bovine luteinized follicular cysts and corpora lutea of estrous cycle: A quantitative histochemical study

Boos, Alois; Peukert-Adam, I; Meyer, Wilfried; Schwarz, Rudolf; Grünert, E

doi:10.1016/0378-4320(87)90038-8

Cited by 8 publications

(7 citation statements)

References 26 publications

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“…In this situation, reduced oocyte-follicle coupling may promote premature luteinisation (el-Fouly et al 1970). In support of this idea, oocytectomised murine OCCs show reduced expression of glycolysis components and, during bovine luteal development, activities of key pentose phosphate pathway and Krebs cycle enzymes increase (Boos et al 1988), implying that a greater proportion of glucose is metabolised through these pathways, reducing lactate output.…”

Section: Discussionmentioning

confidence: 65%

“…In consequence, glycolytic ATP production also increased with a marked increase in energy production after ovulation induction in vitro. Several energy-demanding processes requiring ATP are known to be triggered by ovulation induction, such as progesterone production (Peluso 2006) and luteal cell differentiation (Boos et al 1988). Glucose is also necessary for cumulus expansion (Sutton-McDowall et al 2004) and purine synthesis (Downs et al 1998).…”

Section: Discussionmentioning

confidence: 99%

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Carbohydrate metabolism by murine ovarian follicles and oocytes grown in vitro

Harris¹,

Adriaens²,

Leese³

et al. 2007

Reproduction

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Metabolic markers are potentially valuable for assessment of follicle development in vitro. Carbohydrate metabolism of murine preantral follicles grown to maturity over 13 days in vitro has been measured, and metabolism of resulting oocyte-cumulus complexes (OCCs) and denuded oocytes has been compared with in vivo ovulated control counterparts. Spent follicle culture media were analysed for glucose, lactate and pyruvate concentrations. During follicle in vitro growth, glycolysis accounted for a rise from w24 to 60% of all glucose consumed. Ovulation induction caused a significant increase in glucose uptake and lactate production by in vitrogrown follicles to 71.7G1.2 and 96.6G4.8 nmoles/day respectively. OCCs grown in vitro had significantly higher rates of glucose consumption and lactate and pyruvate production (110.1G3.5, 191.8G8.9 and 31.7G1.7 pmoles/h respectively) than in vivo ovulated controls (67.4G8.1, 113.9G17.1 and 20.2G4.0 pmoles/h respectively), but a reduced capacity for pyruvate consumption (1.13G0.06 vs 1.49G0.06 pmoles/h by in vivo ovulated oocytes). Metabolism of OCCs was affected by the quality of the original follicle. In vitro-grown oocytes had a reduced cytoplasmic volume when compared with controls (168.3G2.0 vs 199.0G3.2 proportionately respectively) but a similar rate of metabolism per unit volume. Meiotic status influenced metabolism of both OCCs and denuded oocytes. In conclusion, glucose consumption and lactate production by cultured follicles increased in tandem with developmental progression and were stimulated prior to ovulation. Additionally, the metabolic profiles of in vitro produced OCCs and the oocytes within them are affected by long-term exposure to the culture environment. Reproduction (2007) 134 415-424

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Carbohydrate metabolism by murine ovarian follicles and oocytes grown in vitro

Harris¹,

Adriaens²,

Leese³

et al. 2007

Reproduction

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“…However, in the absence of LH, the number of 3OHSDpositive luteal cells in control cultures decreased by day 7, suggesting that LH maintains 3flHSD activity in luteal cells during long-term culture. 3flHSD, which is the enzyme that converts pregnenolone to progesterone, has been identified in luteal tissues of several species (Boos et al 1988;Caffrey et al 1979;Jablonh-Shariff et al 1993;Sasano et al 1990;Schwall et al 1986). In rats, hCG increased 3OHSD activity and total ovarian content of 3flHSD d N A (bbrie et al 199 1, 1992).…”

Section: Discussionmentioning

confidence: 99%

Proliferation and progesterone production of ovine luteal cells from several stages of the estrous cycle: effects of fibroblast growth factors and luteinizing hormone

Grazul-Bilska¹,

Redmer²,

Jablonka‐Shariff³

et al. 1995

Can. J. Physiol. Pharmacol.

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This study was conducted to evaluate the effects of fibroblast growth factor 1 (FGF-1), fibroblast growth factor 2 (FGF-2), or luteinizing hormone (LH) on proliferation and progesterone secretion of ovine luteal cells from days 5, 10, or 15 after estrus (estrus = day 0; n = 4 or 5 ewes/day). After enzymatic dispersion, luteal cells were incubated in the presence or absence of various doses of FGF-1, FGF-2, LH, or fetal bovine serum (FBS) (positive control) in serum-free media for 7 days in 24-well plates. Cells were counted on day 7 of culture and media analyzed for progesterone concentration. For all treatments, maximal effects (Emax) and dissociation constants (KD) were calculated. In addition, luteal cells were cultured in eight-chamber slides and treated as above, but on day 7 of culture cells were fixed and stained for the presence of 3beta-hydroxy-delta 5-steroid dehydrogenase (3beta HSD). The number of steroidogenic (3beta HSD positive) cells per unit area was counted for control cultures (no treatment) and cultures treated with the most effective doses of FGF-1, FGF-2, LH, OR FBS in proliferation and (or) progesterone assays. FGF-1, FGF-2, AND FBS stimulated (p < 0.05) proliferation of luteal cells from all stages of luteal development in a dose-dependent manner. In addition, LH increased (p < 0.01) the number of 3beta HSD-positive cells across all stages of luteal development. Moreover, LH and FBS increased (p < 0.05) progesterone secretion by luteal cells from all stages in a dose-responsive manner, but the effects of FGF-1 and FGF-2 were variable. For proliferation, the Emax of all factors was greatest (p < 0.01) on day 5, whereas the KD values were similar across days of the estrous cycle. For progesterone production, the Emax and KD of LH and FBS were similar and did not differ across the estrous cycle. These data demonstrate the luteal cells from the early luteal phase of the estrous cycle exhibit the greatest ability to proliferate and (or) increase their progesterone secretion in response to FGF-1, FGF-2, LH, or FBS. In addition, although LH does not affect the total number of luteal cells in culture, it does increase the number of steroidogenic cells. These data indicate that in addition to LH, fibroblast growth factors may be involved in regulation of luteal growth and differentiation in ewes.

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“…The data profiles show that cell area, cell perimeter, cytoplasmic area, and the quotient q of large luteal cells vary depending on the functional activity of these cells, i. e. progesterone secretion: It is reported in the literature that in the clp the progesterone secretion displays a similar pattern as far as concentrations in peripheral blood (10,27,28,29) or in luteal tissues (14) are concerned; the same was also demonstrated in the lfc (1,22,23). The ratio of nuclear area to cytoplasmic area (1 :q) changed in the same way as progesterone concentrations in peripheral blood.…”

Section: Discussionmentioning

confidence: 66%

The Large Luteal Cells of Luteinized Follicular Cysts and Corpora Lutea Periodica in Cows. A Cytometric Study*

Peukert-Adam¹,

Schwarz²,

Boos³

et al. 1987

Journal of Veterinary Medicine Series A

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The ovaries of 40 cows, 20 with luteinized follicular cysts (Ifc) and 20 with corpora lutea periodica (clp), were collected at the Hannover slaughter house. Their h e a l tissues were classified into 5 developmental stages by histological examination. Twenty-five randomly selected large h e a l cells from each of two tissue regions of every Ifc and clp were measured for cell area, cell perimeter, nuclear area, and nuclear perimeter with a microscope morphometer. Additionally, the cytoplasmic area and the ratio of nuclear area to cytoplasmic area (1 : q, q = cytoplasmic area : nuclear area) were calculated.With respect to h e a l tissue development, the data of all variates determined in Ifc and clp showed characteristic profiles. The profiles of cell area, cell perimeter, cytoplasmic area, and the ratio 1 : q paralleled the h e a l cells' steroidogenic activity, as reported in the literature. The ratio 1 : q was the widest when the luteal tissue had reached its maximal development. Presumably it is an expression of the activity of large luteal cells. The large luteal cells of Ifc and clp showed no relevant differences as to the variates determined. The results are discussed with regard to the clinical significance of the lfc. Zusammenfassung Die groi3en Luteinzellen von Follikel-Lutein-Zysten und Corpora lutea periodica beim Rind. Eine zytometrische Studie Auf dem Schlachthof Hannover wurden die Ovarien von 20 Kuhen mit Follikel-Lutein-Zysten (FLZ) und von 20 Kuhen rnit Corpora lutea periodica (Clp) gesammelt. Aufgrund histologischer

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Activity of Δ5-3β-Hydroxysteroid dehydrogenase, succinate dehydrogenase, and glucose-6-phosphate dehydrogenase in bovine luteinized follicular cysts and corpora lutea of estrous cycle: A quantitative histochemical study

Cited by 8 publications

References 26 publications

Carbohydrate metabolism by murine ovarian follicles and oocytes grown in vitro

Carbohydrate metabolism by murine ovarian follicles and oocytes grown in vitro

Proliferation and progesterone production of ovine luteal cells from several stages of the estrous cycle: effects of fibroblast growth factors and luteinizing hormone

The Large Luteal Cells of Luteinized Follicular Cysts and Corpora Lutea Periodica in Cows. A Cytometric Study*

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