Proliferation and progesterone production of ovine luteal cells from several stages of the estrous cycle: effects of fibroblast growth factors and luteinizing hormone

Grazul-Bilska, Anna T.; Redmer, Dale A.; Jablonka‐Shariff, Albina; Biondini, Michela; Reynolds, Lawrence P.

doi:10.1139/y95-062

Cited by 43 publications

(18 citation statements)

References 47 publications

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“…Responsiveness to LH and dbcAMP demonstrates that cells used in this study were functional. These stimulatory effects of LH and/or dbcAMP on progesterone production by luteal cells have also been demonstrated previously in sheep (Niswender & Nett 1994, Grazul-Bilska et al 1995, Niswender et al 2000.…”

Section: Overallsupporting

confidence: 80%

Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro

Borowczyk

Johnson²,

Bilski³

et al. 2007

Reproduction

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To evaluate the role of gap junctions in the regulation of progesterone secretion, two experiments were conducted. In Experiment 1, luteal cells obtained on days 5, 10, and 15 were cultured overnight at densities of 50!10 3 , 100!10 3 , 300!10 3 , and 600! 10 3 cells/dish in medium containing: (1) no treatment (control), (2) LH, or (3) dbcAMP. In Experiment 2, luteal cells from days 5and 10 of the estrous cycle were transfected with siRNA, which targeted the connexin (Cx) 43 gene. In Experiment 1, progesterone secretion, Cx43 mRNA expression, and the rates of gap junctional intercellular communication (GJIC), were affected by the day of the estrous cycle, cell density, and treatments (LH or dbcAMP). The changes in progesterone secretion were positively correlated with the changes in Cx43 mRNA expression and the rates of GJIC. Cx43 was detected on the luteal cell borders in every culture, and luteal cells expressed 3b-hydroxysteroid dehydrogenase. In Experiment 2, two Cx43 gene-targeted sequences decreased Cx43 mRNA expression and progesterone production by luteal cells. The changes in Cx43 mRNA expression were positively correlated with changes in progesterone concentration in media. Thus, our data demonstrate a relationship between gap junctions and progesterone secretion that was supported by (1) the positive correlations between progesterone secretion and Cx43 mRNA expression and GJIC of luteal cells and (2) the inhibition of Cx43 mRNA expression by siRNA that resulted in decreased production of progesterone by luteal cells. This suggests that gap junctions may be involved in the regulation of steroidogenesis in the ovine corpus luteum.

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Section: Overallsupporting

confidence: 80%

Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro

Borowczyk

Johnson²,

Bilski³

et al. 2007

Reproduction

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“…MMP-2 cleaves fibroblast growth factor (FGF) receptors [43], and FGF-2 increases progesterone production in luteal cell culture [44]. The proliferative capabilities of FGFs have also been demonstrated in CL of a variety of species, including sheep [45,46].…”

Section: Discussionmentioning

confidence: 99%

Matrix Metalloproteinase Expression and Activity Following Prostaglandin F2α-Induced Luteolysis1

Ricke

Smith

2002

Biology of Reproduction

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Luteal tissue contains matrix metalloproteinases (MMPs) that cleave specific components of the extracellular matrix (ECM) and are inhibited by tissue inhibitors of metalloproteinases (TIMPs). We previously reported a decrease in luteal TIMP-1 within 15 min of prostaglandin F(2 alpha) (PGF(2 alpha))-induced luteolysis. An increase in the MMP:TIMP ratio may promote ECM degradation and apoptosis, as observed in other tissues that undergo involution. The objectives of these experiments were to determine whether 1) PGF(2 alpha) affects expression of mRNA encoding fibrillar collagenases (MMP-1 and -13), gelatinases A and B (MMP-2 and -9), membrane type (mt)-1 MMP (MMP-14), stromelysin (MMP-3), and matrilysin (MMP-7), and 2) PGF(2 alpha) increases MMP activity during PGF(2 alpha)-induced luteolysis in sheep. Corpora lutea (n = 3-10/time point) were collected at 0, 15, and 30 min and 1, 2, 4, 6, 12, 24, and 48 h after PGF(2 alpha) administration. Northern blot analysis confirmed the presence of all MMPs except MMP-9. Expression of mRNA for the above MMPs (except MMP-2) increased significantly (P < 0.05) by 30 min, and all MMPs increased significantly (P < 0.05) by 6 h after PGF(2 alpha) administration. Expression of MMP-14 mRNA increased significantly (P < 0.05) by 15 min post-PGF(2 alpha) and remained elevated through 48 h. MMP activity in luteal homogenates (following proenzyme activation and inactivation of inhibitors) was increased significantly (P < 0.05) by 15 min and remained elevated through 48 h post-PGF(2 alpha). MMP activity was localized (in situ zymography) to the pericellular area of various cell types in the 0-h group and was markedly increased by 30 min post-PGF(2 alpha). MMP mRNA expression and activity were significantly increased following PGF(2 alpha) treatment. Increased MMP activity may promote ECM degradation during luteolysis.

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“…FGF-2 act directly on the secretory function of bovine luteal cells [52] and may be a potential luteotropic factor in this tissue during the estrous cycle and pregnancy. In sheep FGF-1, FGF-2 and LH stimulated proliferation and progesterone production of luteal cells especially from the early luteal phase [53]. In sheep CL presence of FGFR-1 and FGFR-2 were evaluated by using Western analysis, immunohistochemistry and topical autoradiography.…”

Section: Fgf In Bovine Corpus Luteummentioning

confidence: 99%

Angiogenic Factors (VEGF, FGF and IGF) in the Bovine Corpus Luteum.

Schams

Berisha

2002

Journal of Reproduction and Development

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Abstract. Angiogenesis, the development of the new capillaries by endothelial cell proliferation and outgrowth from pre-existing vessels, is one of the prominent features of early corpus luteum (CL). The process of angiogenesis is an important component of normal development and function of CL. Of the numerous promoters of angiogenesis and maintenance of new established capillaries that have been identified, the most important factors appear to be vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF). The biological effects of VEGF and FGF factor families are mediated by signaling through specific tyrosine kinase receptors. The VEGF, FGF and IGF family members in bovine CL are discussed with the literature available for large domestic animals. The highest expression for VEGF, VEGFR-2, FGF-2, FGFR, IGF-1, IGF-2 and IGFR1 were found during the early luteal phase (Ld 1-4) followed by a significant decrease afterwards. The VEGF and IGF-1 protein was localized predominantly in luteal cells. FGF-1 mRNA expression peaked (P<0.05) during mid-luteal stage and FGF-1 protein was localized in cytoplasm of luteal cells, but endothelial cells are always negative. FGF-2 protein during angiogenesis (d 1-5) was found in cytoplasm of endothelial cells and changed thereafter exclusively to the cytoplasm of luteal cells. In contrast, the IGF-2 protein is exclusively localized in pericytes and few endothelial cells. The results obtained (mainly in ruminant) suggest an important role of these growth factors for angiogenesis and furthermore for maintenance and function of the bovine corpus luteum.

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Proliferation and progesterone production of ovine luteal cells from several stages of the estrous cycle: effects of fibroblast growth factors and luteinizing hormone

Cited by 43 publications

References 47 publications

Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro

Role of gap junctions in regulation of progesterone secretion by ovine luteal cells in vitro

Matrix Metalloproteinase Expression and Activity Following Prostaglandin F2α-Induced Luteolysis1

Angiogenic Factors (VEGF, FGF and IGF) in the Bovine Corpus Luteum.

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