Activity of ozonated water and ozone against Staphylococcus aureus and Pseudomonas aeruginosa biofilms

Abstract

SummaryBackgroundThe known bactericidal properties of ozone have not been checked in relation to its action on bacterial biofilms. This is especially true of ozonated fluids. The aim of this study was to investigate the bactericidal activity of ozonated water and that of a mixture of ozone and oxygen against biofilms.Material/MethodsEighteen clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa exhibiting various levels of antibiotic sensitivity were investigated. Bacteria were cultured in biofilm form on polystyrene titration plates for periods of 2 to 72 hours. The biofilms formed in this way were exposed to in statu nascendi ozonated water produced in a prototype device that had been tested in clinical conditions, or to a mixture of oxygen and ozone generated in the same device. Live cells in the biofilm were stained with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide solution. The degree of reduction of viable bacteria following ozone exposure was determined.ResultsOzonated water was found to be an effective bactericidal agent against biofilms after as little as 30 seconds of exposure, while the bactericidal activity of the ozone-oxygen solution was much lower. Prolongation of the duration of biofilm exposure to the gaseous disinfectant to 40 minutes led to a reduction in the viable cell count, which nevertheless remained high.ConclusionsUnlike the ozone-oxygen mixture, ozonated water effectively destroys bacterial biofilms in vitro.

Antibacterial effect of gaseous and aqueous ozone in root canals contaminated with Pseudomonas aeruginosa

“…Consequently, although aqueous ozone achieved the complete elimination of P. aeruginosa, a few bacteria were detected in the gaseous ozone application in root canals. This outcome of the present study showed better results than the previous studies' (Bialoszewski et al, 2011;Huth et al, 2009;Case et al, 2012) results because of the higher concentration and longer application time for the disinfection of root canals.…”

“…Moreover, only a few studies have investigated the antibacterial effects of ozone against P. aeruginosa in endodontics. Particularly, aqueous (Bialoszewski et al, 2011) and gaseous (Bialoszewski et al, 2011;Huth et al, 2009;Case et al, 2012) ozone were emphasized against P. aeruginosa in root canals. Furthermore, this subject has attracted the attention of researchers.…”

“…Furthermore, this subject has attracted the attention of researchers. For instance, Bialoszewski et al (2011) investigated the bactericidal efficiacy of aqueous ozone in root canals infected by P. aeruginosa. Aqueous ozone was found to be effective after at least 30 s of application.…”

“…Therefore, aqueous ozone was useful in reducing the number of oral microorganisms for the treatment of endodontic infections (Hems et al, 2005). Some studies reported the antibacterial effect of aqueous ozone against a number of P. aeruginosa (gram-positive bacteria) bacteria in infected root canals (Huth et al, 2005;Bialoszewski et al, 2011).…”

Bacterial biofilms in food processing environments: a review of recent developments in chemical and biological control

“…aeruginosa (Bialoszewski et al ., ) or L. monocytogenes (Nicholas et al ., ) although ozonated water is effective in reducing or removing biofilms of Pseudomonas spp. (Tachikawa et al ., ; Bialoszewski et al ., ), Staph. aureus (Cabo et al ., ; Bialoszewski et al ., ) and S .…”