Activity ofSenna villosaagainstTrypanosoma cruzi

Guzmán, Eduardo Lozano; Gonzalez, Roger Medina; Flôres, Simone Hickmann; Zavala, Jorge Tay; Rosado, M. E.; Pérez, Salud

doi:10.3109/13880200490514069

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…The antiprotozoal activity was observed at all doses evaluated (2.1, 8.4 and 33.6 µg/g) (Figure 1), but the antiprotozoal activity against the amastigote forms was only observed when higher doses (8.4 and 33.6 µg/g) were used (Figure 2). The antiprotozoal activity against T. cruzi found in the present study is similar to the in vitro activity previously reported by Guzman et al (2004Guzman et al ( , 2008.…”

Section: Discussionsupporting

confidence: 92%

“…The antiprotozoal activity of the methanol, aqueous, and chloroform extracts of S. villosa against T. cruzi have also been previously reported in vitro (Guzman et al, 2004). In another study, (8-hydroxymethylen)trieicosanyl acetate displayed antiprotozoal activity against the epimastigote and trypomastigote forms of T. cruziin vitro (Guzman et al, 2008).…”

Section: Discussionmentioning

confidence: 57%

“…The mixture was refluxed for 4 h and filtered (Guzman et al, 2004). The solvent was evaporated, and the residue was chromatographed on silica gel (70-230 mesh) and eluted with hexane, while increasing the polarity with ethyl acetate.…”

Section: Extraction and Isolation Of (8-hydroxymethylen)trieicosanyl mentioning

confidence: 99%

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Anti-trypanosomal activity of (8-hydroxymethylen)-trieicosanyl acetate against infective forms of Trypanosoma cruzi

Jiménez‐Coello

Acosta-Viana

Guzmán-Marín

et al. 2010

Pharmaceutical Biology

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The activity of an (8-hydroxymethylen)-trieicosanyl acetate compound obtained from chloroform extracts of Senna villosa (Mill.) H.S. Irwin & Barneby (Leguminosae) against Trypanosoma cruzi was evaluated in vivo. Oral doses of 2.1, 8.4, and 33.6 microg/g were tested for 28 days in BALB/c mice infected with T. cruzi. Reduced parasitemia levels of 70.5%, 73.8%, and 80.9%, respectively, were observed. A significant reduction in amastigote nests was detected in the cardiac tissue of treated animals at doses of 8.4 and 33.6 microg/g. The LD50 of (8-hydroxymethylen)-trieicosanyl acetate was impossible to determine because none of the animals died, even at oral doses of 5000 microg/g; consequently, it was impossible to determine the acute oral toxicity in vivo.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 57%

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Anti-trypanosomal activity of (8-hydroxymethylen)-trieicosanyl acetate against infective forms of Trypanosoma cruzi

Jiménez‐Coello

Acosta-Viana

Guzmán-Marín

et al. 2010

Pharmaceutical Biology

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“…The phytochemical analysis has shown the existence of alkaloids, sterols, flavonoids, and two 9, 10 anthraquinones (Mena et al 1997). Crude extracts (methanol, aqueous and chloroform) from S. villosa leaves has been previously tested and a strong antiprotozoal activity in vitro against epimastigote forms of T. cruzi was found in chloroform extract at concentration of 1.6 µg/ mL (Guzmán et al 2004). From the chloroformic extract, the compound (8-hydroxymethylen)-trieicosanyl acetate was isolated which demonstrated antiprotozoal activity against epimastigotes and trypomastigotes of T. cruzi under in vitro conditions (at concentrations of 1.65, 3.3 and 6.6 µg/mL) (Guzman et al 2008) and antitrypanosomal activity in vivo at 33.6 µg/g in mice which were treated 24 hours after inoculation (Jimenez-Coello et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Antitrypanosomal Activity of Senna villosa in Infected Balb/C Mice with Trypanosoma Cruzi during the Sub Acute Phase of Infection

Jiménez‐Coello¹,

Guzmán-Marín²,

Pérez-Gutiérrez³

et al. 2011

Afr. J. Trad. Compl. Alt. Med.

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Antitrypanosomal activity of chloroform extract of Senna villosa leaves was evaluated in the sub acute phase of mice infected with Trypanosoma cruzi. Oral doses of 3.3, 6.6 and 13.2 µg/g were tested during 15 days on infected mice BALB/c, beginning treatment 40 days after infection to evaluate specifically the antitrypanosomal activity over the amastigote form of the parasite. Two different amount of parasites (100 and 500) were inoculated to 25 mice for each doses tested. At the end of the assay the animals were sacrificed and cardiac and skeletal tissue sections were stained with hematoxylin-eosin (HE) for identification and quantification of amastigote nest. In mice infected with 100 parasites, a significant reduction in the number of amastigote nest was observed in cardiac tissue of treated animals at all doses evaluated (p<0.05). An important reduction of amastigote nest was also observed in treated animals and infected with 500 parasites in comparison with no treated mice or treated with allopurinol. RésuméActivité antitrypanosomal de chloroforme extrait de feuilles Senna villosa a été évaluée dans la sous phase aiguë de souris infectées par Trypanosoma cruzi. Des doses orales de 3,3, 6,6 et 13,2 µg / g ont été testés pendant 15 jours sur des souris infectées BALB /c, le début du traitement 40 jours après l'infection d'évaluer précisément l'activité antitrypanosomal sur la forme amastigote du parasite. Deux montant différent de parasites (100 et 500) ont été inoculés à 25 souris pour chaque doses testées. À la fin de l'essai les animaux ont été sacrifiés et des coupes de tissus cardiaques et squelettiques ont été colorées à l'hématoxyline-éosine (HE) pour l'identification et la quantification du nid amastigote. Chez les souris infectées avec 100 parasites, une réduction significative du nombre de nids amastigote a été observée dans le tissu cardiaque des animaux traités à toutes les doses évaluées (p <0,05). Une réduction importante du nid amastigote a également été observée chez les animaux traités et infectés avec 500 parasites par rapport à pas de souris traitées ou traitées avec l'allopurinol.

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In vitro and in vivo trypanocidal activity of native plants from the Yucatan Peninsula

et al. 2011

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Ethanol extracts of Senna villosa, Serjania yucatanensis, Byrsonima bucidaefolia, and Bourreria pulchra were evaluated for their in vitro activity against epimastigotes and trypomastigotes of Trypanosoma cruzi. Results showed that the leaf extracts of S. yucatanensis and B. pulchra were the most active against epimastigotes (IC(100) = 100 μg/mL) and trypomastigotes of T. cruzi (95% or more reduction in the number of parasites at 100 and 50 μg/mL). However, only the leaf extract of S. yucatanensis showed significant trypanocidal activity when tested in vivo, reducing 75% of the parasitemia in infected mice at 100 mg/kg. This same extract inhibited the egress of trypomastigotes from infected cells and proved not to be cytotoxic (IC(50) = 318.8 ± 2.3 μg/mL).

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Activity ofSenna villosaagainstTrypanosoma cruzi

Cited by 7 publications

References 13 publications

Anti-trypanosomal activity of (8-hydroxymethylen)-trieicosanyl acetate against infective forms of Trypanosoma cruzi

Anti-trypanosomal activity of (8-hydroxymethylen)-trieicosanyl acetate against infective forms of Trypanosoma cruzi

Antitrypanosomal Activity of <i>Senna villosa</i> in Infected Balb/C Mice with Trypanosoma Cruzi during the Sub Acute Phase of Infection

In vitro and in vivo trypanocidal activity of native plants from the Yucatan Peninsula

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